Zebrafish can regenerate endoskeleton in larval pectoral fin but the regenerative ability declines

Yoshida, Kazuhide; Kawakami, Koichi; Abe, Gembu; Tamura, Koji

doi:10.1016/j.ydbio.2020.04.010

Cited by 12 publications

(20 citation statements)

References 71 publications

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“…Indeed, gene expression studies in paired and median ns have identi ed a similar pattern in the expression of developmental genes, such as the nested expression of Hox genes 19,35,48−50 . We found that prdm16 is expressed in dorsal n mesenchyme by observations of EGFP expressions in the gt1116A line, and this gene is also expressed in pectoral n mesenchyme at the early embryonic stage 34,51,52 . Our and previous studies indicate that prdm16-positive mesenchymal cells differentiate into skeletal elements in both pectoral and dorsal ns 34 .…”

Section: Differences Between Median Ns and Paired Ns In Developmentmentioning

confidence: 74%

“…In gt1116A, the gene-trapping gal4 construct was integrated within the prdm16 gene, and the UAS:EGFP reporter was found to be expressed in mesenchymal cell populations of the early pectoral n bud 33,34 . In addition, UAS:EGFP in gt1116A has been reported to be expressed in other developing median ns, including the dorsal n 34 . We rst examined whether EGFP expression patterns of gt1116A are valid as a reporter of the n mesenchyme in the developing dorsal n primordium.…”

Section: Resultsmentioning

confidence: 99%

“…An analysis of the gt1116A line suggested that the development of the dorsal n primordial mesenchyme is associated with the protrusion of the LMFF, which raises the question of whether cell proliferation in the n mesenchyme may contribute to dorsal n primordium development. To identify the distribution of proliferating cells in the dorsal n primordium, we performed wholemount immunohistochemistry with an anti-phospho histone H3 (pH3) antibody 30,34 . In 4.5-5.0 mm SL zebra sh (n=6) (Fig.…”

Section: Cell Proliferation In Dorsal N Mesenchymementioning

confidence: 99%

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Developmental Independence of Median Fins From the larval Fin Fold Revises Their Evolutionary Origin

Miyamoto

Kawakami

Tamura

et al. 2022

Preprint

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The median fins in modern fish that show separated forms (dorsal, anal, and caudal fins) are derived from a continuous fold-like structure, both in ontogeny and phylogeny. The median fin fold (MFF) hypothesis assumes that the median fins evolved by reducing some positions in the continuous fin fold of basal chordates, based on the classical morphological observation of developmental reduction in the larval fin folds of living fish. However, the developmental processes of median fins are still unclear at the cellular and molecular levels. Here, we describe the transition from the larval fin fold into the median fins in zebrafish at the cellar and molecular developmental level. We demonstrate that reduction does not play a role in the emergence of the dorsal fin primordium: the reduction occurs passively after the primordium formation, rather than actively scrapping the non-fin forming region by inducing cell death. We also report that the emergence of specific mesenchymal cells and their proliferation promote the dorsal fin primordium formation. Based on these results, we propose a revised hypothesis of median fin evolution in which acquisition of de novo developmental mechanisms is a crucial evolutionary component of the separated forms of median fins.

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Section: Differences Between Median Ns and Paired Ns In Developmentmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Cell Proliferation In Dorsal N Mesenchymementioning

confidence: 99%

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Developmental Independence of Median Fins From the larval Fin Fold Revises Their Evolutionary Origin

Miyamoto

Kawakami

Tamura

et al. 2022

Preprint

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“…Meanwhile, the canonical AEC marker fgf8 is absent in zebrafish caudal fin epidermis covering the amputation plane ( Shibata et al, 2016 ). Interestingly, zebrafish pectoral fin amputations were suggested to form the AER due to re-expression of basal epidermal rspo2 after amputations, which is also used to detect fgf8 expressing AER ( Yoshida et al, 2020 ). For X. laevis tail regeneration, the expression pattern of fgf8 is conflicted in different reports: while colorimetric in situ hybridizations detect fgf8 in the AEC with inconsistent patterns ( Beck et al, 2006 ; Lin and Slack, 2008 ; Okumura et al, 2019 ), bulk-RNA-Seq and scRNA-Seq based results suggest fgf8 is not expressed in the Xenopus tail AEC ( Aztekin et al, 2019 ; Okumura et al, 2019 ).…”

Section: Divergent Molecular Cellular and Tissue-level Properties Of Wound Epidermis And Apical Epithelial Cap In Other Regeneration Modementioning

confidence: 99%

Tissues and Cell Types of Appendage Regeneration: A Detailed Look at the Wound Epidermis and Its Specialized Forms

Aztekin

2021

Front. Physiol.

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Therapeutic implementation of human limb regeneration is a daring aim. Studying species that can regrow their lost appendages provides clues on how such a feat can be achieved in mammals. One of the unique features of regeneration-competent species lies in their ability to seal the amputation plane with a scar-free wound epithelium. Subsequently, this wound epithelium advances and becomes a specialized wound epidermis (WE) which is hypothesized to be the essential component of regenerative success. Recently, the WE and specialized WE terminologies have been used interchangeably. However, these tissues were historically separated, and contemporary limb regeneration studies have provided critical new information which allows us to distinguish them. Here, I will summarize tissue-level observations and recently identified cell types of WE and their specialized forms in different regeneration models.

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“…Of note, two of the reprogramming factors, Lin28 and Prdm16 are re-expressed in blastema of regenerating appendages in other systems (Rao et. al., 2009;Yoshida et. al., 2020).…”

Section: Potential Of Rlpcs For Clinical Applicationmentioning

confidence: 99%

Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

Atsuta

Lee

et al. 2021

Preprint

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The early limb bud consists of mesenchymal progenitors (limb progenitors) derived from the lateral plate mesoderm (LPM) that produce most of the tissues of the mature limb bud. The LPM also gives rise to the mesodermal components of the trunk, flank and neck. However, the mesenchymal cells generated at these other axial levels cannot produce the variety of cell types found in the limb bud, nor can they be directed to form a patterned appendage-like structure, even when placed in the context of the signals responsible for organizing the limb bud. Here, by taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28) normally expressed in the early limb bud, that are capable of imparting limb progenitor-like properties to non-limb fibroblasts. Cells reprogrammed by these factors show similar gene expression profiles, and can differentiate into similar cell types, as endogenous limb progenitors. The further addition of Lin41 potentiates proliferation of the reprogrammed cells while suppressing differentiation. These results suggest that these same four key factors may play pivotal roles in the specification of endogenous limb progenitors.

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Zebrafish can regenerate endoskeleton in larval pectoral fin but the regenerative ability declines

Cited by 12 publications

References 71 publications

Developmental Independence of Median Fins From the larval Fin Fold Revises Their Evolutionary Origin

Developmental Independence of Median Fins From the larval Fin Fold Revises Their Evolutionary Origin

Tissues and Cell Types of Appendage Regeneration: A Detailed Look at the Wound Epidermis and Its Specialized Forms

Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

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