Zebrafish as a Model to Understand Vertebrate Development

Pathak, Narendra; Barresi, Michael

doi:10.1016/b978-0-12-812431-4.00045-2

Cited by 8 publications

(6 citation statements)

References 346 publications

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“…The expansion in the use of fish models is well illustrated by the increase in the number of zebrafish facilities globally from an estimated 667 in 2011 (Lawrence et al ., 2012) to 1581 in 2023 (http://www.zfin.org). Today, zebrafish models are used by scientists in a wide range of disciplines including embryology (Pathak & Barresi, 2020), physiology (Kwong, Kumai & Perry, 2014), neuroscience (Hall et al ., 2014), behaviour (McArthur, Chow & Fetcho, 2020), epigenetics (Cavalieri & Spinelli, 2017), ecotoxicology (Bambino & Chu, 2017), disease biology (Bailone et al ., 2020) and the effects of climate change (Pinheiro et al ., 2021). The ability to create genetically selected and/or manipulated zebrafish lines has furthermore enabled significant advances in precision for ecotoxicology (Cassar et al ., 2020), disease processes (Adamson, Sheridan & Grierson, 2018) and human drug validation studies (Winter et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Paull,

Lee,

Tyler

2023

Biological Reviews

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Reproducibility in animal research is impacted by the environment, by husbandry practices in the laboratory and by the animals' provenance. These factors, however, are often not adequately considered by researchers. A disconnect between researchers and animal care staff can result in inappropriate housing and husbandry decisions for scientific studies with those animals. This is especially the case for the research in neuro‐behaviour, epigenetics, and the impact of climate change, as heritable phenotypic, behavioural or physiological changes are known to result from the animals' environmental housing, husbandry, provenance and prior experience. This can lead to greater variation (even major differences) in data outcomes among studies, driving scientific uncertainties. Herein, we illustrate some of the endpoints measured in fish studies known to be intrinsically linked to the environment and husbandry conditions and assess the significance of housing and husbandry practice decisions for research adopting these endpoints for different fish species. We highlight the different priorities and challenges faced by researchers and animal care staff and how harmonising their activities and building greater understanding of how husbandry practices affect the fish will improve reproducibility in research outcomes. We furthermore illustrate how improving engagement between stakeholders, including regulatory bodies, can better underpin fish husbandry decisions and where researchers could help to drive best husbandry practices through their own research with fish models.

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Section: Introductionmentioning

confidence: 99%

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Paull,

Lee,

Tyler

2023

Biological Reviews

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“…This study sought to establish a baseline understanding of the varied cell types in the developing forebrain that may provide important commissural axon growth architectures and guidance cues. We took advantage of the zebrafish model system's accessibility for high resolution microscopic analysis and relatively simple embryonic brain architecture as compared to its mammalian vertebrate counterparts (Pathak & Barresi, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Vertebrate brain development in particular involves the creation of a diverse array of cell types, formation of complex and interacting neural circuits, and multidimensional cell migration with tissue level morphogenesis. The relatively simple vertebrate model system of the zebrafish, Danio rerio, has provided a particularly tractable system to probe the mechanisms of brain development because it offers direct and transparent access to the early developing embryo with the powerful advantages of genetic manipulation (Pathak and Barresi, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Schnabl

Litz

Schneider

et al. 2021

Developmental Neurobiology

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Development of the central nervous system (CNS) is a complex process that requires the coordinated generation of the correct number, position, and differentiation of neuronal and glial lineages. Vertebrate brain development involves the creation of a diverse array of cell types, the formation of complex neural circuits, and cell migration with tissue level morphogenesis (Kuzmicz-Kowalska & Kicheva, 2020;Lindsey et al., 2018;Namba & Huttner, 2017). The zebrafish, Danio rerio, enables direct and transparent access to the early embryo and possesses growing stockpiles of genetic tools to

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“…Post 7 days of fertilization, the organism would be able to swim, whereas the organism is said to be fully matured and able to reproduce by week 10. All organisms will develop ovaries before being differentiated into male gonads [19]. Figure 1 summarizes the developmental stages of zebrafish.…”

Section: Biology Of Zebrafishmentioning

confidence: 99%

Zebrafish as a Model System to Study the Mechanism of Cutaneous Wound Healing and Drug Discovery: Advantages and Challenges

et al. 2021

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In humans, cutaneous wounds may heal without scars during embryogenesis. However, in the adult phase, the similar wound may undergo a few events such as homeostasis, blood clotting, inflammation, vascularization, and the formation of granulation tissue, which may leave a scar at the injury site. In consideration of this, research evolves daily to improve the healing mechanism in which the wound may heal without scarring. In regard to this, zebrafish (Danio rerio) serves as an ideal model to study the underlying signaling mechanism of wound healing. This is an important factor in determining a relevant drug formulation for wound healing. This review scrutinizes the biology of zebrafish and how this favors the cutaneous wound healing relevant to the in vivo evidence. This review aimed to provide the current insights on drug discovery for cutaneous wound healing based on the zebrafish model. The advantages and challenges in utilizing the zebrafish model for cutaneous wound healing are discussed in this review. This review is expected to provide an idea to formulate an appropriate drug for cutaneous wound healing relevant to the underlying signaling mechanism. Therefore, this narrative review recapitulates current evidence from in vivo studies on the cutaneous wound healing mechanism, which favours the discovery of new drugs. This article concludes with the need for zebrafish as an investigation model for biomedical research in the future to ensure that drug repositions are well suited for human skin.

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Zebrafish as a Model to Understand Vertebrate Development

Cited by 8 publications

References 346 publications

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Beyond compliance: harmonising research and husbandry practices to improve experimental reproducibility using fish models

Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Zebrafish as a Model System to Study the Mechanism of Cutaneous Wound Healing and Drug Discovery: Advantages and Challenges

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