Zebrafish and Medaka: new model organisms for modern biomedical research

Lin, Cheng Yung; Chiang, Cheng Yi; Tsai, Huai Jen

doi:10.1186/s12929-016-0236-5

Cited by 140 publications

(90 citation statements)

References 120 publications

(134 reference statements)

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“…[56][57][58] About 70% of all human genes have orthologues in zebrafish, while 65% of human proteins linked to diseases have similar counterparts in Drosophila. [56][57][58] About 70% of all human genes have orthologues in zebrafish, while 65% of human proteins linked to diseases have similar counterparts in Drosophila.…”

Section: Developing New Models For Cpmentioning

confidence: 99%

The genetic basis of cerebral palsy

Fahey

MacLennan

Kretzschmar

et al. 2017

Develop Med Child Neuro

159

123

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Although prematurity and hypoxic-ischaemic injury are well-recognized contributors to the pathogenesis of cerebral palsy (CP), as many as one-third of children with CP may lack traditional risk factors. For many of these children, a genetic basis to their condition is suspected. Recent findings have implicated copy number variants and mutations in single genes in children with CP. Current studies are limited by relatively small patient numbers, the underlying genetic heterogeneity identified, and the paucity of validation studies that have been performed. However, several genes mapping to intersecting pathways controlling neurodevelopment and neuronal connectivity have been identified. Analogous to other neurodevelopmental disorders such as autism and intellectual disability, the genomic architecture of CP is likely to be highly complex. Although we are just beginning to understand genetic contributions to CP, new insights are anticipated to serve as a unique window into the neurobiology of CP and suggest new targets for intervention.

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Section: Developing New Models For Cpmentioning

confidence: 99%

The genetic basis of cerebral palsy

Fahey

MacLennan

Kretzschmar

et al. 2017

Develop Med Child Neuro

159

123

View full text Add to dashboard Cite

show abstract

“…Given its many advantages, zebrafish is now a recognized model for toxicological and biomedical assays [74][75][76][77]. The main advantages of this species are rapid development, external fertilization, easy observation of all developmental stages, small size, transparency of the embryos and larvae, large number of embryos, ease of maintenance and close contact with surrounding medium (water) allowing easy interface with materials.…”

Section: Zebrafish As a Model For Testing The Toxicity Of Spions And mentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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The lack of toxicological data on nanomaterials makes it difficult to assess the risk related to their exposure, and as a result further investigation is required. This chapter presents the synthesis of controlled oxide nanoparticles followed by the evaluation of their safety profile or toxicity (iron, titanium and zinc oxides). The controlled surface chemistry, dispersion in several media, morphology and surface charge of these nanoparticles are presented (transmission electron microscopy, dynamic light scattering, zeta potential, X-ray photoelectron spectroscopy). Classical cytotoxic and cellular uptake studies on different cancer cell lines from liver, prostate, heart, brain and spinal cord are discussed. The incidence of nanoparticles on biogenesis and activity of cell organelles is also highlighted, as well as their biodistribution in animal models. The acute toxicity on zebrafish embryo model is also presented. Finally, the stress is put on the influence and the necessity of controlling the protein corona, a layer of plasma proteins physically adsorbed at the surface of such nanoparticles as a result of their presence in the bloodstream (or relevant biological fluids).

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“…Spawns are generally large (average 100 embryos) and embryos can be raised to (sexual) maturity within 2–3 months, which is less labor-intensive and cheaper than for other commonly used laboratory animals. Also, routine techniques used in genetic research, including knock-in, knockdown, and knockout, are well-established in zebrafish (Lin, Chiang, & Tsai, 2016). Hence, zebrafish are particularly suitable vertebrates for high-frequency mutagenesis and large-scale genetic screening studies.…”

Section: Advantageous Properties Of Zebrafish To Study Geneticsmentioning

confidence: 99%

Studying disorders of vertebrate iron and heme metabolism using zebrafish

Vorm

Paw

2017

The Zebrafish - Disease Models and Chemical Screens

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Iron is a crucial component of heme- and iron-sulfur clusters, involved in vital cellular functions such as oxygen transport, DNA synthesis, and respiration. Both excess and insufficient levels of iron and heme-precursors cause human disease, such as iron-deficiency anemia, hemochromatosis, and porphyrias. Hence, their levels must be tightly regulated, requiring a complex network of transporters and feedback mechanisms. The use of zebrafish to study these pathways and the underlying genetics offers many advantages, among others their optical transparency, ex-vivo development and high genetic and physiological conservations. This chapter first reviews well-established methods, such as large-scale mutagenesis screens that have led to the initial identification of a series of iron and heme transporters and the generation of a variety of mutant lines. Other widely used techniques are based on injection of RNA, including complementary morpholino knockdown and gene overexpression. In addition, we highlight several recently developed approaches, most notably endonuclease-based gene knockouts such as TALENs or the CRISPR/Cas9 system that have been used to study how loss of function can induce human disease phenocopies in zebrafish. Rescue by chemical complementation with iron-based compounds or small molecules can subsequently be used to confirm causality of the genetic defect for the observed phenotype. All together, zebrafish have proven to be – and will continue to serve as an ideal model to advance our understanding of the pathogenesis of human iron and heme-related diseases and to develop novel therapies to treat these conditions.

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Zebrafish and Medaka: new model organisms for modern biomedical research

Cited by 140 publications

References 120 publications

The genetic basis of cerebral palsy

The genetic basis of cerebral palsy

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Studying disorders of vertebrate iron and heme metabolism using zebrafish

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