Zebrafish: An In Vivo Model for Nano EHS Studies

Lin, Sijie; Zhao, Yan; Nel, André E.; Lin, Shuo

doi:10.1002/smll.201202115

Cited by 146 publications

(96 citation statements)

References 97 publications

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“…To date, various animal models are in principle available to investigate and dissect the relevance of the NPs' biomolecule corona for human health. These are ranging from rodents to fish and other organisms, and have been reviewed before (17,19,31,(131)(132)(133)(134)(135). Notably, recent developments also applied successfully HTS/HCS approaches for whole animals, such as zebrafish (121,136).…”

Section: Systematic Strategies To Dissect the Impact Of Nm-coronas Onmentioning

confidence: 99%

The bio-corona and its impact on nanomaterial toxicity

Westmeier

Chen²,

Stauber

et al. 2015

European Journal of Nanomedicine

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Abstract:The rapidly growing application of nanosized materials and nano-scaled processes will result in increased exposure of humans and the environment. The small size of nanomaterials (NM) comparable with molecular building blocks of cells raises concerns that their toxic potential cannot be extrapolated from studies of larger particles due to their unique physico-chemical properties. These properties are also responsible that NM rapidly adsorb various (bio)molecules when introduced into complex physiological or natural environments. As the thus formed protein/biomolecule 'corona' seems to affect the NM' in situ identity, an understanding of its toxicological relevance and the biophysical forces regulating corona formation is needed but not yet achieved. This review introduces our current concept of corona formation and evolution and present analytical methods for corona profiling. We discuss toxicity mechanisms potentially affected by the biomolecule corona, including NM cellular uptake and impact on components of the blood system. Further, we comment on pending knowledge gaps and challenges, which need to be resolved by the field. We conclude by presenting a tiered systems biology-driven approach recommended to mechanistically understand the coronas' nanotoxicological relevance and predictive potential.

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Section: Systematic Strategies To Dissect the Impact Of Nm-coronas Onmentioning

confidence: 99%

The bio-corona and its impact on nanomaterial toxicity

Westmeier

Chen²,

Stauber

et al. 2015

European Journal of Nanomedicine

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“…Furthermore, the body of knowledge for ZF is small compared to that of rodents in terms of metabolism and pharmacokinetics. 85 To our knowledge, no comparative data exists for establishing when is it necessary to induce human tumors on an inherently angiogenic model in order to study angiogenesis. Researchers must determine what is necessary to do this by establishing a correlation between data in these two scenarios.…”

Section: Resultsmentioning

confidence: 99%

“…The NP or sample application step can present a bottleneck, especially if microangiography and injection is the exposure method of choice. 85 Automated high-throughput injection techniques are being developed 86 87 but have thus far only been applied to early embryo injection 87 and for injecting into non-vascular areas of ZF larvae. 86 However, the injection location can be altered by designing a specific macro for the software control program, 86 thus vascular injections will likely be tested soon.…”

Section: High-throughput Zf Assaysmentioning

confidence: 99%

Testing Nanoparticles for Angiogenesis-Related Disease: Charting the Fastest Route to the Clinic

Sarsons¹,

Tekrony²,

Yaehne³

et al. 2014

j biomed nanotechnol

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Recently, nanoparticles (NPs) have been established as ideal drug delivery vehicles for treating cancer. This is due to the enhanced permeability and retention (EPR) effect that is a direct result of the angiogenic nature of the tumor tissue and its ability to sequester chemotherapeutics from healthy tissues. Ideal drug delivery nanocarriers will exploit the EPR effect, accumulate in the tumorous tissue, and be able to release the drugs at a high concentration where needed, thereby reducing undesirable side effects. In order to determine ideal NP qualities that enable drugs to be delivered in such a manner, extensive testing in biological systems is required. However, it is impractical to study new potential nanocarriers in humans or in mammalian models due to the potential adverse consequences, low throughput, and high cost. Simpler models would allow for higher throughput screening of nanocarrier vehicles. This review outlines the most recent advances in alternative model assays and their significance in testing NPs en route to the clinic. In decreasing complexity, we examine zebrafish embryos, the chorioallantoic membrane of the chicken embryo, multicell static and flow-based assays, and single cell assays for efficacy, accuracy and utility as predictors for human therapeutic outcomes.

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“…The ZF is considered the gold standard for evaluating environmental toxicity (Scholz et al, 2008). With the expansion of nanotechnology, the ZF has been gaining ground as an experimental model for environmental health and safety tests of various nanomaterial (Lin et al, 2013;Scholz et al, 2008). The ZF model has been used for assessing the toxicity of silver nanoparticles (Muth-Köhne et al, 2013), silica (Duan et al, 2013) and other nanomaterials (Jevgenij et al, 2013).…”

Section: Toxicological Studies On Studies On Zebrafish Modelmentioning

confidence: 99%

“…This fact promotes and increases the relevance of their use in biological assays, which is hard to be matched in vitro (Lin et al, 2013). The objective of this work is to update knowledge on the use of ZF as an experimental model for biological assays with natural products and derived.…”

Section: Introductionmentioning

confidence: 99%

Use of zebrafish (Danio rerio) in experimental models for biological assay with natural products

Santos¹,

Duarte²,

Fernandes³

et al. 2016

Afr. J. Pharm. Pharmacol.

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Research on natural products is facing significant difficulty. The analysis "high-throughput screening" has limited effectiveness in their evaluation. This report on in vitro screening of pharmacological activity of the drugs candidate molecule by evaluating a single target. The presence of many substances in a natural extract makes this process unprecise. The zebrafish (Danio rerio, ZF) is well suited to highthroughput applications owing to its high fecundity, rapid extrauterine development and transparency during organogenesis. This fact promotes increase in the relevance of their use in biological assays, which is hard to be matched in vitro. Tests based on ZF can aid in the isolation of bioactive molecules from plant extracts, which was identified in a large-scale screening. This increases the biological relevance of such findings. Another decisive factor for the use of ZF as an experimental model, for widespread testing, is the few extracts or the isolated compounds required for tests. Therefore, the growing number of publications and innovative models created for the research shows a lot of diseases with this species, revealing the importance of ZF as an experimental model for the screening of natural products.

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Zebrafish: An In Vivo Model for Nano EHS Studies

Cited by 146 publications

References 97 publications

The bio-corona and its impact on nanomaterial toxicity

The bio-corona and its impact on nanomaterial toxicity

Testing Nanoparticles for Angiogenesis-Related Disease: Charting the Fastest Route to the Clinic

Use of zebrafish (Danio rerio) in experimental models for biological assay with natural products

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