Zeb2 Regulates Myogenic Differentiation in Pluripotent Stem Cells

Filippo, Ester Sara Di; Costamagna, Domiziana; Giacomazzi, Giorgia; Cortés-Calabuig, Álvaro; Stryjewska, Agata; Huylebroeck, Danny; Fulle, Stefania; Sampaolesi, Maurilio

doi:10.3390/ijms21072525

Cited by 9 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the same time, hsa-miR-192-5p reduces the TGF-β1-induced inflammatory response and apoptosis in tendon cells [ 26 ]. ZEB2, a predicted target for hsa-miR-192-5p, is expressed in murine skeletal muscle tissue and has an important role in the skeletal muscle differentiation of pluripotent stem cells and in muscle tissue regeneration from adult myogenic progenitors [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Serum-Circulating microRNAs in Sporadic Inclusion Body Myositis

Lucchini

Arcangelis

Santoro

et al. 2023

IJMS

View full text Add to dashboard Cite

Background: Sporadic inclusion body myositis (s-IBM) represents a unique disease within idiopathic inflammatory myopathies with a dual myodegenerative–autoimmune physiopathology and a lack of an efficacious treatment. Circulating miRNA expression could expand our knowledge of s-IBM patho-mechanisms and provide new potential disease biomarkers. To evaluate the expression of selected pre-amplified miRNAs in the serum of s-IBM patients compared to those of a sex- and age-matched healthy control group, we enrolled 14 consecutive s-IBM patients and 8 sex- and age-matched healthy controls. By using two different normalization approaches, we found one downregulated and three upregulated miRNAs. hsa-miR-192-5p was significantly downregulated, while hsa-miR-372-3p was found to be upregulated more in the s-IBM patients compared to the level of the controls. The other two miRNAs had a very low expression levels (raw Ct data > 29). hsa-miR-192-5p and hsa-miR-372-3p were found to be significantly dysregulated in the serum of s-IBM patients. These miRNAs are involved in differentiation and regeneration processes, thus possibly reflecting pathological mechanisms in s-IBM muscles and potentially representing disease biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

Serum-Circulating microRNAs in Sporadic Inclusion Body Myositis

Lucchini

Arcangelis

Santoro

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Interestingly, two significant additive SNPs within or nearby VRTN was found in the current study to be associated with LMD. In addition, the Zinc Finger E-Box Binding Homeobox 2 ( ZEB2 ) gene was proved to play a role in skeletal muscle differentiation in pluripotent stem cells [ 53 ]. Although it has not been reported in pigs, it would be worth seeking to verify it in the future.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study Reveals Additive and Non-Additive Effects on Growth Traits in Duroc Pigs

Xue

Liu

et al. 2022

Genes

View full text Add to dashboard Cite

Growth rate plays a critical role in the pig industry and is related to quantitative traits controlled by many genes. Here, we aimed to identify causative mutations and candidate genes responsible for pig growth traits. In this study, 2360 Duroc pigs were used to detect significant additive, dominance, and epistatic effects associated with growth traits. As a result, a total number of 32 significant SNPs for additive or dominance effects were found to be associated with various factors, including adjusted age at a specified weight (AGE), average daily gain (ADG), backfat thickness (BF), and loin muscle depth (LMD). In addition, the detected additive significant SNPs explained 2.49%, 3.02%, 3.18%, and 1.96% of the deregressed estimated breeding value (DEBV) variance for AGE, ADG, BF, and LMD, respectively, while significant dominance SNPs could explain 2.24%, 13.26%, and 4.08% of AGE, BF, and LMD, respectively. Meanwhile, a total of 805 significant epistatic effects SNPs were associated with one of ADG, AGE, and LMD, from which 11 sub-networks were constructed. In total, 46 potential genes involved in muscle development, fat deposition, and regulation of cell growth were considered as candidates for growth traits, including CD55 and NRIP1 for AGE and ADG, TRIP11 and MIS2 for BF, and VRTN and ZEB2 for LMD, respectively. Generally, in this study, we detected both new and reported variants and potential candidate genes for growth traits of Duroc pigs, which might to be taken into account in future molecular breeding programs to improve the growth performance of pigs.

show abstract

“…The role of the so-called EMT-TFs is not restricted to epithelial cells. These transcription factors are abundantly expressed, and play essential roles in, several other lineages including neurons [361][362][363] , myocytes 364,365 , adipocytes 366 , haematopoietic cells [367][368][369] and melanocytes 370,371 . Strikingly, the functions of downstream targets identified in these lineages are largely overlapping with those observed during classical EMT; genes involved in cell adhesion, migration, chemotaxis and growth factor receptors.…”

Section: Emt-tfs Beyond Epithelial Plasticitymentioning

confidence: 99%

The epithelial–mesenchymal plasticity landscape: principles of design and mechanisms of regulation

2023

View full text Add to dashboard Cite

Epithelial-mesenchymal plasticity (EMP) enables cells to interconvert between several states across the epithelial-mesenchymal landscape, thereby acquiring hybrid epithelial/mesenchymal phenotypic features. This plasticity is crucial for embryonic development and wound healing, but also underlies the acquisition of several malignant traits during cancer progression. Recent research using systems biology and single-cell profiling methods has provided novel insights into the main forces that shape EMP, which include the microenvironment, lineage specification and cell identity, and the genome. Additionally, key roles have emerged for hysteresis (cell memory) and cellular noise, which can drive stochastic transitions between cell states.Here, we review these forces and the distinct but interwoven layers of regulatory control that stabilize EMP states or facilitate epithelial-mesenchymal transitions (EMTs) and discuss the therapeutic potential of manipulating the EMP landscape.

show abstract

Zeb2 Regulates Myogenic Differentiation in Pluripotent Stem Cells

Cited by 9 publications

References 38 publications

Serum-Circulating microRNAs in Sporadic Inclusion Body Myositis

Serum-Circulating microRNAs in Sporadic Inclusion Body Myositis

Genome-Wide Association Study Reveals Additive and Non-Additive Effects on Growth Traits in Duroc Pigs

The epithelial–mesenchymal plasticity landscape: principles of design and mechanisms of regulation

Contact Info

Product

Resources

About