Zearalenone promotes follicle development through activating the SIRT1/PGC-1α signaling pathway in the ovaries of weaned gilts

Wan, Boyang; Huang, Libo; Jing, Changwei; Yang, Li; Jiao, Ning; Ming, Liang; Jiang, Shuzhen; Yang, Weiren

doi:10.1093/jas/skac058

Cited by 12 publications

(11 citation statements)

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“…However, a previous study showed that the positive detection rate of ZEN could reach 69.15%, and the highest value of ZEN in compound feed samples was 4.33 mg/kg [ 6 ]. In our study, significantly increased serum ZEN, α-ZOL, and β-ZOL levels of weaned piglets were observed in the piglets fed the diet containing 3.0 mg/kg ZEN, which was in line with our previous study [ 25 ]. Zhang et al [ 24 ] found that 970 μg/kg ZEN could increase the levels of ZEN, α-ZOL, and β-ZOL of serum in gilts.…”

Section: Discussionsupporting

confidence: 93%

Quantitative Proteomic Analysis of Zearalenone-Induced Intestinal Damage in Weaned Piglets

Jiang²,

Lü³

et al. 2022

Toxins

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Zearalenone (ZEN), also known as the F-2 toxin, is a common contaminant in cereal crops and livestock products. This experiment aimed to reveal the changes in the proteomics of ZEN-induced intestinal damage in weaned piglets by tandem mass spectrometry tags. Sixteen weaned piglets either received a basal diet or a basal diet supplemented with 3.0 mg/kg ZEN in a 32 d study. The results showed that the serum levels of ZEN, α-zearalenol, and β-zearalenol were increased in weaned piglets exposed to ZEN (p < 0.05). Zearalenone exposure reduced apparent nutrient digestibility, increased intestinal permeability, and caused intestinal damage in weaned piglets. Meanwhile, a total of 174 differential proteins (DEPs) were identified between control and ZEN groups, with 60 up-regulated DEPs and 114 down-regulated DEPs (FC > 1.20 or <0.83, p < 0.05). Gene ontology analysis revealed that DEPs were mainly involved in substance transport and metabolism, gene expression, inflammatory, and oxidative stress. The Kyoto Encyclopedia of Genes and Genomes analysis revealed that DEPs were significantly enriched in 25 signaling pathways (p < 0.05), most of which were related to inflammation and amino acid metabolism. Our study provides valuable clues to elucidate the possible mechanism of ZEN-induced intestinal injury.

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Section: Discussionsupporting

confidence: 93%

Quantitative Proteomic Analysis of Zearalenone-Induced Intestinal Damage in Weaned Piglets

Jiang²,

Lü³

et al. 2022

Toxins

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“…With the increase of ZEA (0.5, 1 and 1.5 mg/kg), the ovarian size increased first and then decreased [ 42 ]. Wan et al [ 43 ] observed the increased quantity of atretic growing follicles, but a greater volume and quantity of healthy growing follicles in the deep ovarian cortex with an increasing level of dietary ZEA (0, 0.15, 1.5 and 3.0 mg/kg). The absence of a difference in estradiol and progesterone in this study might be attributed to that the estradiol and progesterone secreted by healthy growing follicles in the deep ovarian cortex could offset the insufficient secretion of atretic growing follicles.…”

Section: Discussionmentioning

confidence: 99%

“…ZEA, α-ZOL, and β-ZOL were detected in serum after pre-pubertal gilts were fed ZEA at doses of 5, 10 and 15 μg /kg BW for 21 and 42 days [ 48 ]. The contents of ZEA, α-ZOL, and β-ZOL in serum of ZEA treatment pigs (doses at 0.15, 1.5 and 3 mg/kg) were higher than those in control pigs [ 43 ]. Zöllner et al [ 44 ] reported that ZEA and its metabolites (α-ZOL and β-ZOL) were detected in urine, muscle tissue and liver of pigs after feeding them mycotoxin-contaminated oats.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, Xiong et al [ 52 ] reported that CKM was highly expressed in skeletal muscle and myocardium, and its gene expression was increased after muscle cell differentiation. Wan et al [ 43 ] reported that ZEA could promote follicle development via the SIRT1/PGC-1α signaling pathway related to energy metabolism. MME was a membrane-bound metallopeptidase whose main functions involved immune response and cell proliferation [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

“…The 0.4 g frozen uterine sample in 5 mL sterile centrifuge tube was ground with a high-throughput tissue grinder (Scientz-192, Beyotime, Shanghai, China). The homogenate was centrifuged at 10,000× g at 4 °C for 10 min, and the supernatant was separated to detect ZEA, α-ZOL, and β-ZOL by LC-MS/MS at the Institute of Quality Standards and Detection Technology of the Chinese Academy of Agricultural Sciences, according to Wan et al [ 43 ] and Zhang et al [ 77 ]. The LC-MS/MS analysis was performed using Agilent 1200 liquid chromatograph (Agilent Technologies, Palo Alto, CA, USA) installed with the 3200 QTrap ® mass spectrometry system (Applied Biosystems, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Quantitative Proteomic Analysis of Zearalenone Exposure on Uterine Development in Weaned Gilts

Liu

Wang

Jiang³

et al. 2022

Toxins

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The aim of this study was to explore the effect of zearalenone (ZEA) exposure on uterine development in weaned gilts by quantitative proteome analysis with tandem mass spectrometry tags (TMT). A total of 16 healthy weaned gilts were randomly divided into control (basal diet) and ZEA3.0 treatments groups (basal diet supplemented with 3.0 mg/kg ZEA). Results showed that vulva size and uterine development index were increased (p < 0.05), whereas serum follicle stimulation hormone, luteinizing hormone and gonadotropin-releasing hormone were decreased in gilts fed the ZEA diet (p < 0.05). ZEA, α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) were detected in the uteri of gilts fed a 3.0 mg/kg ZEA diet (p < 0.05). The relative protein expression levels of creatine kinase M-type (CKM), atriopeptidase (MME) and myeloperoxidase (MPO) were up-regulated (p < 0.05), whereas aldehyde dehydrogenase 1 family member (ALDH1A2), secretogranin-1 (CHGB) and SURP and G-patch domain containing 1 (SUGP1) were down-regulated (p < 0.05) in the ZEA3.0 group by western blot, which indicated that the proteomics data were dependable. In addition, the functions of differentially expressed proteins (DEPs) mainly involved the cellular process, biological regulation and metabolic process in the biological process category. Some important signaling pathways were changed in the ZEA3.0 group, such as extracellular matrix (ECM)-receptor interaction, focal adhesion and the phosphoinositide 3-kinase–protein kinase B (PI3K-AKT) signaling pathway (p < 0.01). This study sheds new light on the molecular mechanism of ZEA in the uterine development of gilts.

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SIRT1 pathway in Parkinson’s disease: a faraway snapshot but so close

et al. 2022

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Silent information regulator (SIRT) has distinctive enzymatic activities and physiological functions to control cell-cycle progression, gene expression, and DNA stability by targeting histone and non-histone proteins. SIRT1 enhances synaptic formation and synaptic activity, and therefore, can reduce the progression of various degenerative brain diseases including Parkinson’s disease (PD). SIRT1 activity is decreased by aging with a subsequent increased risk for the development of degenerative brain diseases. Inhibition of SIRT1 promotes inflammatory reactions since SIRT1 inhibits transcription of nuclear factor kappa B (NF-κB) which also inhibits SIRT1 activation via activation of microRNA and miR-34a which reduce NAD synthesis. SIRT1 is highly expressed in microglia as well as neurons, and has antioxidant and anti-inflammatory effects. Therefore, this review aimed to find the possible role of SIRT1 in PD neuropathology. SIRT1 has neuroprotective effects; therefore, downregulation of SIRT1 during aging promotes p53 expression and may increase the vulnerability of neuronal cell deaths. PD neuropathology is linked with the sequence of inflammatory changes and the release of pro-inflammatory cytokines due to the activation of inflammatory signaling pathways. In addition, oxidative stress, inflammatory disorders, mitochondrial dysfunction, and apoptosis contribute mutually to PD neuropathology. Thus, SIRT1 and SIRT1 activators play a crucial role in the mitigation of PD neuropathology through the amelioration of oxidative stress, inflammatory disorders, mitochondrial dysfunction, apoptosis, and inflammatory signaling pathways.

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Zearalenone promotes follicle development through activating the SIRT1/PGC-1α signaling pathway in the ovaries of weaned gilts

Cited by 12 publications

References 50 publications

Quantitative Proteomic Analysis of Zearalenone-Induced Intestinal Damage in Weaned Piglets

Quantitative Proteomic Analysis of Zearalenone-Induced Intestinal Damage in Weaned Piglets

Quantitative Proteomic Analysis of Zearalenone Exposure on Uterine Development in Weaned Gilts

SIRT1 pathway in Parkinson’s disease: a faraway snapshot but so close

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