Zearalenone induced embryo and neurotoxicity in zebrafish model (Danio rerio): Role of oxidative stress revealed by a multi biomarker study

Muthulakshmi, Sellamani; Maharajan, Kannan; Habibi, Hamid R.; Kadirvelu, K.; Mudili, Venkataramana

doi:10.1016/j.chemosphere.2018.01.141

Cited by 107 publications

(46 citation statements)

References 54 publications

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“…Fungi plays a substantial role in spoilage of agricultural commodities and produces a variety of toxic secondary metabolites called mycotoxins that are harmful to humans and farm animals (Andersen and Thrane, 2006 ; Van Egmond et al, 2007 ; Mudili et al, 2014 ; Venkataramana et al, 2014 ; Muthulakshmi et al, 2018 ). The fungal infestations primarily commence at pre-harvesting and post-harvesting times owed to inappropriate agronomic practices (Neme and Mohammed, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Detoxification Efficacy of Irradiation on Zearalenone Mycotoxin in Various Fruit Juices by Response Surface Methodology and Elucidation of Its in-vitro Toxicity

2018

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Fruits are vital portion of healthy diet owed to rich source of vitamins, minerals, and dietary fibers, which are highly favorable in keeping individual fit. Unfortunately, these days, one-third of fruits were infested with fungi and their toxic metabolites called mycotoxins, which is most annoying and pose significant health risk. Therefore, there is a need to suggest appropriate mitigation strategies to overcome the mycotoxins contamination in fruits. In the present study, detoxification efficiency of irradiation on zearalenone (ZEA) mycotoxin was investigated in distilled water and fruit juices (orange, pineapple, and tomato) applying statistical program response surface methodology (RSM). The independent factors were distinct doses of irradiation and ZEA, and response factor was a percentage of ZEA reduction in content. A central composite design (CCD) consists of 13 experiments were planned applying software program Design expert with distinct doses of irradiation (up to 10 kGy) and ZEA (1–5 μg). The results revealed that independent factors had a positive significant effect on the response factor. The analysis of variance (ANOVA) was followed to fit a proper statistical model and suggested that quadratic model was appropriate. The optimized model concluded that doses of irradiation and ZEA were the determinant factors for detoxification of ZEA in fruit juices. Further, toxicological safety of irradiation mediated detoxified ZEA was assessed in the cell line model by determining the cell viability (MTT and live/dead cell assays), intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), nuclear damage, and caspase-3 activity. The higher level of live cells and MMP, lower extent of intracellular ROS molecules and caspase-3, and intact nuclear material were noticed in cells treated with irradiation mediated detoxified ZEA related to non-detoxified ZEA. The results confirmed that toxicity of ZEA was decreased with irradiation treatment and detoxification of ZEA by irradiation is safe. The study concluded that irradiation could be a potential post-harvest food processing technique for detoxification of ZEA mycotoxin in fruit juices. However, irradiation of fruit juices with high dose of 10 kGy has minimally altered the quality of fruit juices.

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Section: Introductionmentioning

confidence: 99%

Assessment of Detoxification Efficacy of Irradiation on Zearalenone Mycotoxin in Various Fruit Juices by Response Surface Methodology and Elucidation of Its in-vitro Toxicity

2018

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“…Several studies have addressed the toxic effects of DON and ZEA in in-vitro and in-vivo experiments and documented their genotoxicity, hepatotoxicity, neurotoxicity, immunotoxicity, nephrotoxicity, reproductive, and developmental toxicity, carcinogenicity, etc. (Zinedine et al, 2007 ; Venkataramana et al, 2014 ; Schumann et al, 2016 ; Kalagatur et al, 2017 ; Gonçalves et al, 2018 ; Muthulakshmi et al, 2018 ; Reddy et al, 2018 ). Moreover, International Agency for Research on Cancer (IARC) has studied the carcinogenic property of DON and ZEA in laboratory animals and classified as the group 3 carcinogens (IARC, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

Combinational Inhibitory Action of Hedychium spicatum L. Essential Oil and γ-Radiation on Growth Rate and Mycotoxins Content of Fusarium graminearum in Maize: Response Surface Methodology

et al. 2018

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Nowadays, contamination of agricultural commodities with fungi and their mycotoxins is one of the most annoying with regard to food safety and pose serious health risk. Therefore, there is a requisite to propose suitable mitigation strategies to reduce the contamination of fungi and mycotoxins in agricultural commodities. In the present study, combinational inhibitory effect of Hedychium spicatum L. essential oil (HSEO) and radiation was established on growth rate, production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium graminearum in maize grains. The HSEO was obtained from rhizomes by hydrodistillation technique and chemical composition was revealed by GC-MS analysis. A total of 48 compounds were identified and major compounds were 1,8-cineole (23.15%), linalool (12.82%), and β-pinene (10.06%). The discrete treatments of HSEO and radiation were effective in reducing the fungal growth rate and mycotoxins content, and the complete reduction was noticed at 3.15 mg/g of HSEO and 6 kGy of radiation. Response surface methodology (RSM) was applied to evaluate the combinational inhibitory effect of HSEO and radiation treatments on fungal growth rate and mycotoxins content. A total of 13 experiments were designed with distinct doses of HSEO and radiation by central composite design (CCD) of Stat-Ease Design-Expert software. In combinational approach, complete reductions of fungal growth, DON, and ZEA content were noticed at 1.89 mg/g of HSEO and 4.12 kGy of radiation treatments. The optimized design concluded that combinational treatments of HSEO and radiation were much more effective in reducing the fungal growth and mycotoxins content compared to their discrete treatments (p < 0.05). Responses of the design were assessed by second-order polynomial regression analysis and found that quadratic model was well fitted. The optimized design has larger F-value and adequate precision, smaller p-value, decent regression coefficients (R2) and found statistically significant (p < 0.05). In addition, correlation matrix, normal plot residuals, Box-Cox, and actual vs. predicted plots were endorsed that optimized design was accurate and appropriate. The proposed combinational decontamination technique could be highly applicable in agriculture and food industry to safeguard the food and feed products from fungi and mycotoxins.

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“…Apart from agricultural commodities, the ZEA level was also detected in environmental matrices such as soil, drainage water, wastewater effluents, river and lakes of Poland, Italy and the USA with the concentration ranged up to 289 ng l −1 (Bucheli, Muñoz, Azuero‐Holguín, & Ulate, ; Gromadzka, Waśkiewicz, Goliński, & Świetlik, ; Hartmann et al, ; Kolpin et al, ; Maragos, ). Therefore, the exposure of ZEA in aquatic systems implies a possible risk to aquatic organisms and subsequent ecosystems (Muthulakshmi, Maharajan, Habibi, Kadirvelu, & Venkataramana, ).…”

Section: Introductionmentioning

confidence: 99%

“…and the USA with the concentration ranged up to 289 ng l −1 (Bucheli, Muñoz, Azuero-Holguín, & Ulate, 2008;Gromadzka, Waśkiewicz, Goliński, & Świetlik, 2009;Hartmann et al, 2008;Kolpin et al, 2014;Maragos, 2012). Therefore, the exposure of ZEA in aquatic systems implies a possible risk to aquatic organisms and subsequent ecosystems (Muthulakshmi, Maharajan, Habibi, Kadirvelu, & Venkataramana, 2018). Natural or synthetic endocrine disrupting compounds are known to interfere with metabolism and reproductive functions (Golshan et al, 2014;Wang, Li, Chen, & Liu, 2018).…”

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confidence: 99%

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Muthulakshmi

Fallah

Habibi

et al. 2018

J of Applied Toxicology

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In the present study, we aimed to assess the adverse effects of zearalenone (ZEA) at environmentally relevant concentrations (0.5, 1, 5 and 10 μg l ) on hypothalamic-pituitary-gonadal axis associated reproductive function using zebrafish model. ZEA was exposed to female zebrafish for 21 days to assess growth indices such as condition factor, hepatosomatic index, gonadosomatic index and caspase 3 activity. Further, expression of estrogen receptor (ER) α and CYP19a1b genes in the brain, ERα and vitellogenin (Vtg) genes in the liver and follicle-stimulating hormone receptor, luteinizing hormone receptor, ERα, steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase (HSD), 17-βHSD and CYP19a1 genes in the ovary were also investigated. Our results showed that there were no significant changes in the condition factor and hepatosomatic index, whereas a significant (P < .05) reduction in the gonadosomatic index, increase in caspase 3 activities and Vtg expression was observed at higher concentration. However, no significant changes were observed at lower treatment levels. Further, we also observed significant (P < .05) upregulation in ERα, Vtg, luteinizing hormone receptor, steroidogenic acute regulatory protein, 3β-HSD, 17β-HSD, CYP19a1 and CYP19a1b genes in treatment groups with higher levels of ZEA. Moreover, in histopathological examination, we observed oocyte atresia and oocyte membrane detachment in ovaries at the highest concentration. In conclusion, the present study revealed the negative impact of ZEA on zebrafish reproductive system by involvement of the hypothalamic-pituitary-gonadal axis-associated reproductive function.

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Zearalenone induced embryo and neurotoxicity in zebrafish model (Danio rerio): Role of oxidative stress revealed by a multi biomarker study

Cited by 107 publications

References 54 publications

Assessment of Detoxification Efficacy of Irradiation on Zearalenone Mycotoxin in Various Fruit Juices by Response Surface Methodology and Elucidation of Its in-vitro Toxicity

Assessment of Detoxification Efficacy of Irradiation on Zearalenone Mycotoxin in Various Fruit Juices by Response Surface Methodology and Elucidation of Its in-vitro Toxicity

Combinational Inhibitory Action of Hedychium spicatum L. Essential Oil and γ-Radiation on Growth Rate and Mycotoxins Content of Fusarium graminearum in Maize: Response Surface Methodology

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

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