Zea mays L. protein changes in response to potassium dichromate treatments

Labra, Massimo; Gianazza, Elisabetta; Waitt, R.; Eberini, Ivano; Sozzi, Andrea; Regondi, Simona; Grassi, F.; Agradi, E.

doi:10.1016/j.chemosphere.2005.06.062

Cited by 97 publications

(64 citation statements)

References 54 publications

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“…It was found, for example, that the protein level of Met synthase is also significantly increased in barley (Hordeum vulgare) leaves under salt stress (Narita et al, 2004). Met synthase and SAM synthase were also up-regulated in maize plants treated with potassium dichromate (Labra et al, 2006). Moreover, ethylene is an essential signaling molecule in induced resistance responses (Bostock et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

The Molecular Basis of Shoot Responses of Maize Seedlings to Trichoderma harzianum T22 Inoculation of the Root: A Proteomic Approach

2008

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Trichoderma spp. are effective biocontrol agents for several soil-borne plant pathogens, and some are also known for their abilities to enhance systemic resistance to plant diseases and overall plant growth. Root colonization with Trichoderma harzianum Rifai strain 22 (T22) induces large changes in the proteome of shoots of maize (Zea mays) seedlings, even though T22 is present only on roots. We chose a proteomic approach to analyze those changes and identify pathways and genes that are involved in these processes. We used two-dimensional gel electrophoresis to identify proteins that are differentially expressed in response to colonization of maize plants with T22. Up-or down-regulated spots were subjected to tryptic digestion followed by identification using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry and nanospray ion-trap tandem mass spectrometry. We identified 91 out of 114 up-regulated and 30 out of 50 down-regulated proteins in the shoots. Classification of these revealed that a large portion of the up-regulated proteins are involved in carbohydrate metabolism and some were photosynthesis or stress related. Increased photosynthesis should have resulted in increased starch accumulation in seedlings and did indeed occur. In addition, numerous proteins induced in response to Trichoderma were those involved in stress and defense responses. Other processes that were up-regulated were amino acid metabolism, cell wall metabolism, and genetic information processing. Conversely, while the proteins involved in the pathways noted above were generally up-regulated, proteins involved in other processes such as secondary metabolism and protein biosynthesis were generally not affected. Up-regulation of carbohydrate metabolism and resistance responses may correspond to the enhanced growth response and induced resistance, respectively, conferred by the Trichoderma inoculation.

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Section: Discussionmentioning

confidence: 99%

The Molecular Basis of Shoot Responses of Maize Seedlings to Trichoderma harzianum T22 Inoculation of the Root: A Proteomic Approach

2008

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“…In contaminated soils, plants must develop efficient mechanisms to transform the absorbed metals and convert to tolerable forms. When there are excess of metals, related ions induce free radicals formation, resulting in oxidative stress and degradation of photosynthetic pigments, and so leading to depreciation in growth [8] and in biophotonic emission [9]. This fact has been observed in toxicity tests -the intensity and distribution of light emitted by stressed samples shows disturbances in relation to control-samples' data [10].…”

Section: Introductionmentioning

confidence: 94%

Spontaneous Light Emission of Wheat Seedlings With K2Cr2O7

Moraes

Martins

Ramos

et al. 2010

Latin America Optics and Photonics Conference

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“…Peroksiredoksinler gelişim, antioksidant, içsel apoptoz düzenleyicisi (Ichimia et al 1997), hücreler arası sinyal molekülü ve moleküler şaperon (Jang et al 2004) gibi farklı hücresel fonksiyonlara sahiptir. Bu proteinin ekspresyonundaki artış metal stresi altındaki birçok bitki türünde tespit edilmiştir (Labra et al 2006;Wang et al2011;Hossain et al 2012a). …”

Section: Antioksidant Savunma Ve Detoksifikasyon Ile İlişkili Proteinlerunclassified

Heavy Metal Toxicity in Plants: Proteomics Approach

Terzi¹,

Yıldız²

2013

AKU-J. Sci. Eng.

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ÖzetAğır metal kirliliği, insan sağlığını olumsuz etkileyen ve tarımsal verimde kayıplara neden olan önemli çevresel tehlikelerden biridir. Bitki hücresinin en önemli fonksiyonu, kendi savunması için ağır metal stresine karşı cevap vermektir. Ağır metal stresine cevap olarak fonksiyonel genler veya proteinlerin teşhisi stres cevaplarının moleküler mekanizmalarının anlaşılmasında önemli bir basamaktır. Protein ekspresyonu sadece transkripsiyonel seviyede değil, aynı zamanda translasyonel ve post-translasyonel seviyelerde de düzenlenmektedir. Bu nedenle, farklı stres koşulları altında bitki proteomundaki değişimleri araştırmak önemlidir. Genom tarafından eksprese olan proteinlerin geniş ölçekli analizi olarak tanımlanan proteomik, sadece proteomun tamamını tanımlamak için değil, aynı zamanda ağır metal stresi gibi farklı fizyolojik koşullar altındaki proteomların karşılaştırılması için güçlü bir moleküler araçtır. Bu derlemede, proteomik teknolojileri ve bitkilerde farklı metabolik olaylarda rolü olan ağır metal teşvikli proteinler tartışılmıştır. Heavy Metal Toxicity in Plants: Proteomics Approach Key wordsPlants; Heavy Metal; Toxicity; Proteomics; Mass Spectrometry AbstractHeavy metal contamination is one of the major environmental hazards, leading to losses in agricultural yields and harmfully affecting human health. The most crucial function of plant cell is to respond against heavy metal stress for self-defence. The identification of the functional genes or proteins that are involved in responses to heavy-metal stress is a fundamental step in understanding the molecular mechanisms of stress responses. Protein expression is regulated not only at the transcriptional level, but also at the translational and post-translational levels. Therefore, it is crucial to investigate the changes in plant proteome under different stress conditions. Proteomics, defined as the large-scale analysis of proteins expressed by the genome, is not only a powerful molecular tool for describing complete proteome, but also for comparative proteomes under different physiological conditions, such as heavy metal stress. In this review, proteomics technologies, and heavy metal induced proteins which play a role in different metabolic events in plants are discussed.

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Zea mays L. protein changes in response to potassium dichromate treatments

Cited by 97 publications

References 54 publications

The Molecular Basis of Shoot Responses of Maize Seedlings to Trichoderma harzianum T22 Inoculation of the Root: A Proteomic Approach

The Molecular Basis of Shoot Responses of Maize Seedlings to Trichoderma harzianum T22 Inoculation of the Root: A Proteomic Approach

Spontaneous Light Emission of Wheat Seedlings With K2Cr2O7

Heavy Metal Toxicity in Plants: Proteomics Approach

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