ZBTB gene expression in HIV patients: a possible new molecular mechanism of viral control

Arcos-Jiménez, Judith Carolina De; Gonzalez-Hernández, Luz Alicia; Ratkovich-González, Sarah; Sánchez-Reyes, Karina; Alvarez-Zavala, Monserrat; Ruiz-Briseño, Mariana del Rocio; Mosqueda, Juan L.; Ávila‐Ríos, Santiago; Ramos-Solano, Moisés; Andrade-Villanueva, Jaime

doi:10.1007/s00705-020-04854-6

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…Frontiers in Bioinformatics frontiersin.org significantly negatively correlated VL-controlled HAART CpG (cg23237634, p-value = 7.19 × 10 −11 ) is located in the promoter of ZBTB7B, a gene that plays a role in CD4 + T cell differentiation. Expression of this gene may be elevated in "elite controllers" (individuals able to suppress viral loads and remain asymptomatic without antiretroviral therapy) compared to individuals who have not received antiretroviral therapy (De Arcos-Jiménez et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Effects of highly active antiretroviral therapy initiation on epigenomic DNA methylation in persons living with HIV

Zhang,

Sehl,

Shih

et al. 2024

Front. Bioinform.

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Introduction: Highly active antiretroviral therapy (HAART) helps improve some measures of accelerated epigenetic aging in persons living with HIV (PLWH), but its overall impact on the epigenome is not fully understood.Methods: In this study, we analyzed the DNA methylation profiles of PLWH (n = 187) shortly before and approximately 2–3 years after they started HAART, as well as matched seronegative (SN) controls (n = 187), taken at two time intervals. Our aim was to identify specific CpGs and biologic pathways associated with HIV infection and initiation of HAART. Additionally, we attempted to identify epigenetic changes associated with HAART initiation that were independent of HIV-associated changes, using matched HIV seronegative (SN) controls (matched on age, hepatitis C status, and interval between visits) to identify CpGs that did not differ between PLWH and SN pre-HAART but were significantly associated with HAART initiation while being unrelated to HIV viral load. Epigenome-wide association studies (EWAS) on >850,000 CpG sites were performed using pre- and post-HAART samples from PLWH. The results were then annotated using the Genomic Regions Enrichment of Annotations Tool (GREAT).Results: When only pre- and post-HAART visits in PLWH were compared, gene ontologies related to immune function and diseases related to immune function were significant, though with less significance for PLWH with detectable HIV viral loads (>50 copies/mL) at the post-HAART visit. To specifically elucidate the effects of HAART separately from HIV-induced methylation changes, we performed EWAS of HAART while also controlling for HIV viral load, and found gene ontologies associated with transplant rejection, transplant-related diseases, and other immunologic signatures. Additionally, we performed a more focused analysis that examined CpGs reaching genome-wide significance (p < 1 × 10−7) from the viral load-controlled EWAS that did not differ between all PLWH and matched SN controls pre-HAART. These CpGs were found to be near genes that play a role in retroviral drug metabolism, diffuse large B cell lymphoma proliferation, and gastric cancer metastasis.Discussion: Overall, this study provides insight into potential biological functions associated with DNA methylation changes induced by HAART initiation in persons living with HIV.

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Section: Discussionmentioning

confidence: 99%

Effects of highly active antiretroviral therapy initiation on epigenomic DNA methylation in persons living with HIV

Zhang,

Sehl,

Shih

et al. 2024

Front. Bioinform.

View full text Add to dashboard Cite

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“…Furthermore, overexpression of ZBTB4 is associated with prolonged relapse-free survival of patients with cancers above [20][21][22][23]. Furthermore, ZBTB4 has been linked to the development of non-neoplastic diseases such as HIV and Alzheimer's disease [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4

2022

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Purpose MicroRNAs (miRNAs) have already been shown to have a strong correlation with the invasion and metastasis capacity of tumor cells. The present research examined the function of miRNA-576-5p (miR-576-5p) in the development of endometrial cancer (EC). Methods miR-576-5p and ZBTB4 expression in EC and benign endometrial tissues was measured using quantitative real-time PCR (qRT-PCR) and western blot. To evaluate the proliferation ability of tumor cells in vitro, 2,5-diphenyl-2H-tetrazolium bromide (MTT) and colony formation assays were carried out. The effect of miR-576-5p on the proliferation ability of EC cells in vivo was measured by the tumor formation in nude mice. The migration and invasion ability of tumor cells was determined using the transwell assay. To confirm the association between expressions of miR-576-5p and zinc finger and BTB domain containing four (ZBTB4), western blot, qRT-PCR, and luciferase assay were carried out. Results miR-576-5p expression increased significantly in EC samples than in benign endometrial tissues. The level of miR-576-5p was significantly higher in the polymerase ε (POLE) ultramutated subgroup compared to the other three subgroups. High levels of miR-576-5p expression were linked to a shorter progression-free interval time in the copy number high subgroup. Furthermore, upregulated miR-576-5p facilitated EC cell invasion and migration in vitro and promoted the proliferation of EC tumor cell lines both in vitro and in vivo. Moreover, this study showed that the expression of ZBTB4 decreased in patients with EC, and the dual-luciferase reporter assay confirmed that miR-576-5p binds directly to the 3′-UTR of ZBTB4 and inhibits the expression of ZBTB4. An increase in miR-576-5p expression leads to a decrease in the mRNA and protein expression level of ZBTB4. The effects of miR-576-5p can be reversed by overexpression of ZBTB4. Conclusion miR-576-5p promoted proliferation and metastasis capacity of EC cells by inhibiting ZBTB4 expression. We hypothesized that miR-576-5p could be a prospective therapeutic target for EC.

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Tissue gene expression profiles and communication networks inform candidate blood biomarker identification in psoriasis and atopic dermatitis

Soul,

Carlsson,

Hofmann

et al. 2024

Clinical Immunology

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ZBTB gene expression in HIV patients: a possible new molecular mechanism of viral control

Cited by 3 publications

References 28 publications

Effects of highly active antiretroviral therapy initiation on epigenomic DNA methylation in persons living with HIV

Effects of highly active antiretroviral therapy initiation on epigenomic DNA methylation in persons living with HIV

miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4

Tissue gene expression profiles and communication networks inform candidate blood biomarker identification in psoriasis and atopic dermatitis

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