Zath3, a neural basic helix-loop-helix gene, regulates early neurogenesis in the zebrafish

Park, Su-Hyeon; Yeo, Sang-Yeob; Yoo, Kyeong-Won; Hong, Sung-Kook; Lee, Suman; Rhee, Myungchull; Chitnis, Ajay B.; Kim, Cheol‐Hee

doi:10.1016/s0006-291x(03)01353-6

Cited by 33 publications

(36 citation statements)

References 31 publications

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“…6). NeuroD4, like NeuroD, is able to induce neuronal fate under ectopic expression (Lee et al, 1995;Takebayashi et al, 1997) and might cooperate with ngn1 to specify different neuronal identities in a subset of cranial ganglia in zebrafish (Park et al, 2003). We found that neuroD4 was expressed in a pattern similar to ngn1 and neuroD (Fig.…”

Section: Hh Signaling Influences the Balance Of Sag Neuronal Subpopulmentioning

confidence: 60%

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Hedgehog Signaling Governs the Development of Otic Sensory Epithelium and Its Associated Innervation in Zebrafish

Sapède

Pujades²

2010

J. Neurosci.

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The inner ear is responsible for the perception of motion and sound in vertebrates. Its functional unit, the sensory patch, contains mechanosensory hair cells innervated by sensory neurons from the statoacoustic ganglion (SAG) that project to the corresponding nuclei in the brainstem. How hair cells develop at specific positions, and how otic neurons are sorted to specifically innervate each endorgan and to convey the extracted information to the hindbrain is not completely understood. In this work, we study the generation of macular sensory patches and investigate the role of Hedgehog (Hh) signaling in the production of their neurosensory elements. Using zebrafish transgenic lines to visualize the dynamics of hair cell and neuron production, we show that the development of the anterior and posterior maculae is asynchronic, suggesting they are independently regulated. Tracing experiments demonstrate the SAG is topologically organized in two different neuronal subpopulations, which are spatially segregated and innervate specifically each macula. Functional experiments identify the Hh pathway as crucial in coordinating the production of hair cells in the posterior macula, and the formation of its specific innervation. Finally, gene expression analyses suggest that Hh influences the balance between different SAG neuronal subpopulations. These results lead to a model in which Hh orients functionally the development of inner ear towards an auditory fate in all vertebrate species.

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Section: Hh Signaling Influences the Balance Of Sag Neuronal Subpopulmentioning

confidence: 60%

“…Whole-mount in situ hybridization was performed as described previously (Hauptmann and Gerster, 1994). Probes were as follows: atoh1a, atoh1b (Millimaki et al, 2007), ptc1 (Hammond et al, 2003), zath3/neuroD4 (Park et al, 2003), gata3 (Cheng et al, 2007), ngn1, and neuroD (Itoh and Chitnis, 2001).…”

Section: Methodsmentioning

confidence: 99%

Hedgehog Signaling Governs the Development of Otic Sensory Epithelium and Its Associated Innervation in Zebrafish

Sapède

Pujades²

2010

J. Neurosci.

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“…Expression of scrt1a and scrt1b was first detected at 24 hpf and of scrt2 at the 3-somite stage, coinciding with the onset of primary neurogenesis which begins at 10.5 hpf and is complete before 24 hpf (Figs. 2A, B, B′, and B′′) (Mueller and Wullimann, 2003;Park et al, 2003). At the 2-cell and shield stages, no transcripts were detected, indicating that the three scratch genes are only expressed zygotically.…”

Section: Isolation Of Zebrafish Scratch Genesmentioning

confidence: 98%

Neuron-Specific Expression of Scratch Genes during Early Zebrafish Development

Dam

Kim

Moon

et al. 2011

Molecules and Cells

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“…In addition to ngn1, at least three other related bHLH genes with putative proneural function are expressed in territories adjacent to the IZ at the end of gastrulation: the achaete-scute homologues asha and ashb (formerly zash1a, zash1b) (Allende and Weinberg, 2002) and the atonal-related gene neurod4 (previously zath3 and atonal3) (Park et al, 2003;Wang et al, 2003). A comparative expression analysis of these proneural markers with precisely staged embryos showed that expression of asha, ashb and neurod4 within the MH area was initiated slightly later than ngn1.…”

Section: Her5 Activity Is Required To Inhibit the Establishment Of A mentioning

confidence: 99%

Her5 acts as a prepattern factor that blocksneurogenin1andcoe2expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary

et al. 2004

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Neurogenesis in both vertebrates and invertebrates is tightly controlled in time and space involving both positive and negative regulators. We report here that the bHLH factor Her5 acts as a prepattern gene to prevent neurogenesis in the anlage of the midbrain/hindbrain boundary in the zebrafish neural plate. This involves selective suppression of both neurogenin1(ngn1) and coe2 mRNA expression in a process that is independent of Notch signalling, and where inhibition of either ngn1or coe2 expression is sufficient to prevent neuronal differentiation across the midbrain-hindbrain boundary. A ngn1 transgene faithfully responds to Her5 and deletion analysis of the transgene identifies an E-box in a ngn1 upstream enhancer to be required for repression by Her5. Together our data demonstrate a role of Her5 as a prepattern factor in the spatial definition of proneural domains in the zebrafish neural plate, in a manner similar to its Drosophila homologue Hairy.

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Zath3, a neural basic helix-loop-helix gene, regulates early neurogenesis in the zebrafish

Cited by 33 publications

References 31 publications

Hedgehog Signaling Governs the Development of Otic Sensory Epithelium and Its Associated Innervation in Zebrafish

Hedgehog Signaling Governs the Development of Otic Sensory Epithelium and Its Associated Innervation in Zebrafish

Neuron-Specific Expression of Scratch Genes during Early Zebrafish Development

Her5 acts as a prepattern factor that blocksneurogenin1andcoe2expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary

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