ZAP-70 and CD38 expression are independent prognostic factors in patients with B-cell chronic lymphocytic leukaemia and combined analysis improves their predictive value.

Abstract

Abstract:Recently identified biological risk factors in B-cell chronic lymphocytic leukemia (B-CLL) include ZAP-70 and CD38 expression. The present study was conducted to clarify whether a combined analysis could improve predictive impact of these two parameters. We examined the expression of ZAP-70 and CD38 by flow cytometry method in 217 newly diagnosed, consecutive, unselected and well characterized B-CLL patients in relation to laboratory parameters and clinical outcome. We confirmed that both ZAP-70 as well as CD38 were independent of prognostic factors. There was a significant correlation between the percentage of leukemic cells positive for ZAP-70 and the percentage of CD38+CD19+ cells (R=0.629; p=0.000001). Combined analysis of ZAP-70 and CD38 showed concordant results in 158/217 patients (72.8%), while in 59 patients the results were discordant (27.2%). A mean treatment free survival (TFS) was the longest in ZAP-70-CD38-patients (45.6 months, comparing to 13.6 months in ZAP-70+CD38+ group). Also a mean overall survival was the longest in ZAP-70-CD38-patients (224.7 months compared to 77.9 months in ZAP-70+CD38+ patients).

Phenotypic complexity of T regulatory subsets in patients with B-chronic lymphocytic leukemia

“…Previous studies have reported that a combination of CD38 and ZAP-70 is the best predictor of disease progression. 33–35 Using the Spearman’s correlation, we found that both CD45RA + T reg and CD8 T reg were significantly inversely correlated with combined CD38 and ZAP-70 expression (Spearman’s r = −0.55 ( P = 0.02) and Spearman’s r = −0.58 ( P = 0.01), respectively). Using the Mann–Whitney test, we compared the mean of these two populations in two groups CD38 − /ZAP70 − and CD38 + /ZAP70 + , and it was found that the frequency of CD45RA + T regs and CD8 + T regs was significantly different.…”

Comparison of CD38, ZAP70 and hTERT Expression with Known Prognostic Markers in Patients with Chronic Lymphocytic Leukemia During Five-Year Follow- up Period

“…[22][23][24][25][26] Although ZAP70 has been shown as an independent prognosis parameter in many studies, there are controversies for CD38. 27 As it has been suggested by Moreno et al the correlation of IgVH mutations, ZAP70 and CD38 expression with response is unclear and needs further investigation. 28 Besides many studies which have supported the prognostic significance of those markers, there is considerable number of controversial comments as well.…”

Abnormal microRNA expression in the course of hematological malignancies

“…Deletions of 13q14.3, 11q23, and 6q12 and trisomy 12 are common abnormalities associated with CLL. 9 – 11 It has been proven that in patients with deletion of 13q14.3, miR15a and miR16–1 expression is inhibited or at very low levels. MiR15a expression reduced in 25% of patients with CLL, and low expression of miR16-1 reported in 45% of cases.…”