Zanthoxylum bungeanum as a natural pickling spice alleviates health risks in animal-derived foods via up-regulating glutathione S-transferase, down-regulating cytochrome P450 1A

Lactobacillus casei-Derived Postbiotics Elevate the Bioaccessibility of Proteins via Allosteric Regulation of Pepsin and Trypsin and Introduction of Endopeptidases

“…The pH of the final solution was adjusted to 8.55, and the bicinchoninic acid (BCA) Protein Assay Kit (Beyotime Institute of Biotechnology, Shanghai, China) was used for protein quantitation. Dithiothreitol was added at a final concentration of 10 mM to the remaining sample solution and incubated at 56 °C for 1 h to break the disulfide bonds and disrupt the spatial structure of the proteins . After cooled at room temperature, cysteine and histidine were alkylated with 55 mM iodoacetamide (IAM) and allowed to react in the dark for 30 min, to ensure complete denaturation of the protein sample and to maintain the reduced state.…”

“…Dithiothreitol was added at a final concentration of 10 mM to the remaining sample solution and incubated at 56 °C for 1 h to break the disulfide bonds and disrupt the spatial structure of the proteins. 26 After cooled at room temperature, cysteine and histidine were alkylated with 55 mM iodoacetamide (IAM) and allowed to react in the dark for 30 min, to ensure complete denaturation of the protein sample and to maintain the reduced state. The mixture was then transferred to the 10 kDa ultrafiltration tube (Merck, Darmstadt, GER) and centrifuged at 4 °C for 20 min (15,000g).…”

“…As a data-independent acquisition (DIA) method, the generic variable DIA (vDIA) parallelizes all fragmentation of precursor ions within the predefined mass ranges; thus, all fragment ions of all precursor ions are acquired. The quantification performance of vDIA has been applied in many studies, involving realizing high-quality quantification and overcoming the disadvantages mentioned above. , Mechanism profiling of the substance in organisms during digestion and metabolism period is difficult to be characterized using a single model, while the introduction of spatial-omics provides a novel idea for investigating metabolic pathways. However, due to the extensive secondary spectra generated by the precursor ions under collision-induced dissociative fragmentation conditions, efficient calculation of the complex DIA data set scan mode is intricate, which extremely limits the application of DIA–mass spectrometry (DIA-MS) in high-throughput omics data acquisition …”

Spatiotemporal Variation of Residual Cortisol Obstructs Nutrient Acquisition in Animal-Derived Foods by Perturbing Glycerophospholipid Metabolism

“…Investigated lipidomics and proteomics analysis deeply explore the dynamic changes of lipids and proteins in pasteurized milk contaminated with propane-1,2-diol and reveal propane-1,2-diol exposure accelerating degradation of lipids via the allosteric effect and reducing the nutritional value of milk . UHPLC-Q-Orbitrap HRMS-based integrative metabolomics and proteomics analyses revealed Zanthoxylum bungeanum application alleviates health risks in aflatoxin B1-contaminated goat meat via upregulating glutathione S-transferase, downregulating cytochrome P450 1A . The molecular mechanism of propylene glycol-induced lipolysis of the biosynthesis pathway in ultrahigh temperature milk was unraveled combined UHPLC-Q-Orbitrap HRMS-based metabolomics and proteomics …”

“…20 UHPLC-Q-Orbitrap HRMS-based integrative metabolomics and proteomics analyses revealed Zanthoxylum bungeanum application alleviates health risks in aflatoxin B1-contaminated goat meat via upregulating glutathione S-transferase, downregulating cytochrome P450 1A. 21 The molecular mechanism of propylene glycol-induced lipolysis of the biosynthesis pathway in ultrahigh temperature milk was unraveled combined UHPLC-Q-Orbitrap HRMS-based metabolomics and proteomics. 22 The present study integrated lipidomics and proteomics analysis to provide a global view and quantitative information on key components.…”