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Garcia, Jose

doi:10.1016/1076-0512(96)00065-9

Cited by 82 publications

(23 citation statements)

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“…B16 cells are easy to culture in vitro [29, 30] and kojic acid as a positive control [17]. Figure 3 demonstrated that Fa-a and Fa-b significantly reduced cellular tyrosinase activity in B16 melanoma cells in the absence of α -MSH stimulation in the dose-dependent manner.…”

Section: Resultsmentioning

confidence: 99%

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Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells

Yan

Yang

Tian

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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The aim of the present study is to preliminarily investigate the antimelanogenesis effect of Inonotus obliquus extracts by cell-free mushroom tyrosinase assay. It was found that petroleum ether and n-butanol extracts might contain unknown potential tyrosinase inhibitors, while its ethyl acetate extract might contain some unknown accelerators. Six compounds were isolated and their structures were identified by interpretation of NMR data and nicotinic acid was first discovered in Inonotus obliquus. In cells testing, betulin and trametenolic acid decreased tyrosinase activity and melanin content, while inotodiol and lanosterol significantly increased tyrosinase activity and melanin content, showing an AC⁡50 of 9.74 and 8.43 μM, respectively. Nicotinie acid, 3β,22,25-trihydroxy-lanosta-8-ene, had a little or no effect on tyrosinase. Betulin exhibited a mode of noncompetitive inhibition with a K I = K IS of 0.4 μM on tyrosinase activity showing an IC50 of 5.13 μM and being more effective than kojic acid (6.43 μM), and trametenolic acid exhibited a mode of mixed inhibition with a K I of 0.9 μM, K IS of 0.5 μM, and an IC50 of 7.25 μM. We proposed betulin and trametenolic acid as a new candidate of potent tyrosinase inhibitors and inotodiol and lanosterol as accelerators that could be used as therapeutic agent.

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Section: Resultsmentioning

confidence: 99%

“…Then potential components were tested for cellular antityrosinase activity and kinetically analyzed in B16 melanoma cells. Kojic acid, that is well known to be an inhibitor of tyrosinase and melanogenesis, was used as a positive control [17]. …”

Section: Introductionmentioning

confidence: 99%

Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells

Yan

Yang

Tian

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Compounds such as hydroquinone, ascorbic acid derivatives, kojic acid, azelaic acid, corticosteroids, retinoids, arbutin, and others have been reported to show inhibitory efficacy. [21][22][23][24][25][26][27] Most known depigmenting agents with small molecular weights are phenolic compounds and usually they show high cytotoxicity against melanocytes. 28) Phenols such as hydroquinone have been reported to induce irreversible depigmenting effect in melan-a melanocytes.…”

Section: Resultsmentioning

confidence: 99%

Depigmentation of Melanocytes by Isopanduratin A and 4-Hydroxypanduratin A Isolated from <i>Kaempferia pandurata</i> R<small>OXB</small>.

Yoon

Shim

Cho

et al. 2007

Biological & Pharmaceutical Bulletin

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Melanin is the phenolic biopolymer that is responsible for pigmentation. Although melanin plays a crucial role in absorbing free radicals from the cytoplasm and shielding from UV light, the overproduction and accumulation of melanin in the skin could be a serious skin disorder. 1) Current therapies for skin pigmentation disease are unsatisfactory. Thus the search for natural and synthetic chemical agents to modulate the metabolism of pigmentation is of great interest. 2)Melanin biosynthesis occurs in a cascade of enzymatic and spontaneous reactions that convert tyrosine to melanin pigments.3) The initial and rate-limiting step in melanin synthesis, the hydroxylation of tyrosine to dihydroxyphenylalanine (DOPA), is controlled by tyrosinase that is the key enzyme in the process. 4) Since the 1980s, skin cancer researchers have studied the melanin biosynthetic pathways, which led to the development of whitening cosmetics and medicines.5) Arbutin, kojic acid, hydroquinone and its derivatives were developed in 1980s.6) However, the clinical effects of those chemicals are unsatisfactory because of their low activity and cell toxicity. 7) Therefore there is a need for safe and effective whitening agents.Kaempferia pandurata ROXB. is a perennial herb of the Zingiberaceae family mainly cultivated in tropical countries, including Indonesia and Thailand. The fresh rhizome has been used as a food ingredient and as a folk medicine for the treatment of colic, dry cough, rheumatism, musle pain, and as an aphrodisiac.8) Several studies reported various activities of K. pandurata, including antiinflammatory, antitumor, antidiarrhea, antidysentery, antiflatulence, and antiepidermophytid effects. 9) Isopanduratin A and 4-hydroxypanduratin A (Fig. 1) are the typical chalcone compounds found in K. pandurata. Chalcones belong to a group of phenolic compounds in the flavonoid family and widely occur in nature as pigments. This category of flavonoids has a broad bioactive spectrum such as anticancer, antibacterial, antifungal activities, etc. [10][11][12] In previous studies, chalcone compounds in K.pandurata showed anticancer, antioxidative, antimutagenic, and antibacterial activities. [13][14][15] However, their depigmenting effect has not yet been examined. Therefore we focused on evaluating the inhibitory effects of chalcone compounds isolated from K. pandurata on melanin biosynthesis and tyrosinase. Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, South Korea: and c R&D Center, Amore-Pacific Corporation; 314-1, Bora-dong, Kiheung-gu, Yongin-si, Kyounggi-do 449-729, South Korea. Received April 11, 2007; accepted July 20, 2007 This study was carried out to investigate the in vitro effects of isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB. on melanin biosynthesis and tyrosinase activity. Two chalcone compounds, isopanduratin A and 4-hydroxypanduratin A, were isolated from the ethyl acetate fraction of ethanol extract as the active principles. Compared with phenylthiourea (IC 50 ‫3.4...

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“…Several skin hyperpigmentation disorders such as freckles, age spots, melasma, post-inflammatory melanoderma and other hyperpigmentation syndromes are the result of abnormal melanin accumulation [3]. Hence, many tyrosinase inhibitors such as kojic acid [4], arbutin [5] and azelaic acid [6] are used in skin whitening products to prevent or treat abnormal skin pigmentation [7]. However, it has been reported that whitening products with chemical skin depigmenting agents can have significant side effects, including pigmented contact dermatitis caused by kojic acid [8], genotoxicity caused by arbutin [9] and transient erythema or skin irritation caused by azelaic acid [10].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Anti-Melanogenic and Antioxidant Characteristics of Eucalyptus camaldulensis Flower Essential Oil and Determination of Its Chemical Composition

Huang

Lim

et al. 2015

IJMS

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The effects of essential oil from Eucalyptus camaldulensis flowers oil on melanogenesis and the oil’s antioxidant characteristics were investigated. Assays of mushroom and cellular tyrosinase activities and melanin content of mouse melanoma cells were performed spectrophotometrically, and the expression of melanogenesis-related proteins was determined by Western blotting. The possible signaling pathways involved in essential oil-mediated depigmentation were also investigated using specific protein kinase inhibitors. The results revealed that E. camaldulensis flower essential oil effectively suppresses intracellular tyrosinase activity and decreases melanin amount in B16F10 mouse melanoma cells. The essential oil also exhibits antioxidant properties and effectively decreases intracellular reactive oxygen species (ROS) levels. The volatile chemical composition of the essential oil was analyzed with gas chromatography–mass spectrometry (GC/MS). The chemical constituents in the essential oil are predominately oxygenated monoterpenes (34.9%), followed by oxygenated sesquiterpenes (31.8%), monoterpene hydrocarbons (29.0%) and sesquiterpene hydrocarbons (4.3%). Our results indicated that E. camaldulensis flower essential oil inhibits melanogenesis through its antioxidant properties and by down-regulating both mitogen-activated protein kinases (MAPK) and protein kinase A (PKA) signaling pathways. The present study indicates that the essential oil has the potential to be developed into a skin care product.

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Abstract: Both glycolic acid/kojic acid and glycolic acid/hydroquinone topical skin care products are highly effective in reducing the pigment in melasma patients. Both formulations should be available to the dermatologist to satisfy the patient's preferences.

Cited by 82 publications

References 0 publications

Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells

Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells

Depigmentation of Melanocytes by Isopanduratin A and 4-Hydroxypanduratin A Isolated from <i>Kaempferia pandurata</i> R<small>OXB</small>.

Investigation of the Anti-Melanogenic and Antioxidant Characteristics of Eucalyptus camaldulensis Flower Essential Oil and Determination of Its Chemical Composition

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