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Brinig, Mary M.; Register, Karen B.; Ackermann, Mark R.; Relman, David A.

doi:10.1186/gb-2006-7-9-r81

Cited by 25 publications

(9 citation statements)

References 42 publications

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“…bronchiseptica and Bpp hu contain 24 genes (BB0121 to BB0144/ BPP0121 to BPP0144)[ 6 , 27 ], but genes predicted to encode modifications to the polysaccharide backbone ( wbmP , wbmN , wbmM , wbmL , wbmJ , and wbmD ) are less conserved in Bpp ov strain Bpp5 in comparison to those genes in Bpp hu strain 12822 and B . bronchiseptica strain RB50 ( Fig 3A ), consistent with previous CGH analysis [ 28 ]. Also, wbmO and wbmI are predicted to be pseudogenes due to frame-shift mutations, consistent with a prior prediction for wbmI ([ 17 ], Fig 3A ).…”

Section: Resultssupporting

confidence: 91%

“…Also, wbmO and wbmI are predicted to be pseudogenes due to frame-shift mutations, consistent with a prior prediction for wbmI ([ 17 ], Fig 3A ). Additionally, wbmE is completely missing in Bpp5 ( Fig 3A ) and wbmK is replaced by a unique gene, with closest sequence similarity to a gene that encodes a methyltransferase type 11 in other bacteria, such as Wolinella succinogenes ([ 28 ], Fig 3A ). Overall, this comparative analysis of the Bpp5 O-antigen locus suggests that Bpp5 has a novel or defective O-antigen.…”

Section: Resultsmentioning

confidence: 99%

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Host Specificity of Ovine Bordetella parapertussis and the Role of Complement

et al. 2015

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The classical bordetellae are comprised of three subspecies that differ from broad to very limited host specificity. Although several lineages appear to have specialized to particular host species, most retain the ability to colonize and grow in mice, providing a powerful common experimental model to study their differences. One of the subspecies, Bordetella parapertussis, is composed of two distinct clades that have specialized to different hosts: one to humans (Bpphu), and the other to sheep (Bppov). While Bpphu and the other classical bordetellae can efficiently colonize mice, Bppov strains are severely defective in their ability to colonize the murine respiratory tract. Bppov genomic analysis did not reveal the loss of adherence genes, but substantial mutations and deletions of multiple genes involved in the production of O-antigen, which is required to prevent complement deposition on B. bronchiseptica and Bpphu strains. Bppov lacks O-antigen and, like O-antigen mutants of other bordetellae, is highly sensitive to murine complement-mediated killing in vitro. Based on these results, we hypothesized that Bppov failed to colonize mice because of its sensitivity to murine complement. Consistent with this, the Bppov defect in the colonization of wild type mice was not observed in mice lacking the central complement component C3. Furthermore, Bppov strains were highly susceptible to killing by murine complement, but not by sheep complement. These data demonstrate that the failure of Bppov to colonize mice is due to sensitivity to murine, but not sheep, complement, providing a mechanistic example of how specialization that accompanies expansion in one host can limit host range.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Host Specificity of Ovine Bordetella parapertussis and the Role of Complement

et al. 2015

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“…This work confirmed the presence of different Insertion Sequence (IS) elements in different species [5]. Overall, even though these strains have shown diverse phenotypes in the mouse model of infection [2,4,12-15] and were isolated from different hosts, they appear to share similar genomic organization and characteristics.…”

Section: Resultssupporting

confidence: 64%

Comparative genomics of the classical Bordetella subspecies: the evolution and exchange of virulence-associated diversity amongst closely related pathogens

et al. 2012

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Background: The classical Bordetella subspecies are phylogenetically closely related, yet differ in some of the most interesting and important characteristics of pathogens, such as host range, virulence and persistence. The compelling picture from previous comparisons of the three sequenced genomes was of genome degradation, with substantial loss of genome content (up to 24%) associated with adaptation to humans. Results: For a more comprehensive picture of lineage evolution, we employed comparative genomic and phylogenomic analyses using seven additional diverse, newly sequenced Bordetella isolates. Genome-wide single nucleotide polymorphism (SNP) analysis supports a reevaluation of the phylogenetic relationships between the classical Bordetella subspecies, and suggests a closer link between ovine and human B. parapertussis lineages than has been previously proposed. Comparative analyses of genome content revealed that only 50% of the pan-genome is conserved in all strains, reflecting substantial diversity of genome content in these closely related pathogens that may relate to their different host ranges, virulence and persistence characteristics. Strikingly, these analyses suggest possible horizontal gene transfer (HGT) events in multiple loci encoding virulence factors, including O-antigen and pertussis toxin (Ptx). Segments of the pertussis toxin locus (ptx) and its secretion system locus (ptl) appear to have been acquired by the classical Bordetella subspecies and are divergent in different lineages, suggesting functional divergence in the classical Bordetellae.

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“…Over the last four decades, B. pertussis and B. parapertussis genomes have been shown to evolve under vaccination- and disease-induced pressure, adapting to human populations [ 22 , 23 , 25 , 26 , 27 , 28 ]. The genomes of B. pertussis and B. parapertussis contain different insertion sequences, mostly IS 481 for B. pertussis and IS 100 1 for B. parapertussis , and these sequences make a major contribution to the deletion, insertion, or inactivation of genes, as observed in some B. pertussis isolates displaying IS 481 insertions within the prn gene [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Post-Translational Modifications and Cytotoxic Properties of the Adenylate-Cyclase Hemolysin Produced by Various Bordetella pertussis and Bordetella parapertussis Isolates

Bouchez

Douché

Dazas

et al. 2017

Toxins

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Bordetella pertussis and Bordetella parapertussis are the causal agents of whooping cough in humans. They produce diverse virulence factors, including adenylate cyclase-hemolysin (AC-Hly), a secreted toxin of the repeat in toxins (RTX) family with cyclase, pore-forming, and hemolytic activities. Post-translational modifications (PTMs) are essential for the biological activities of the toxin produced by B. pertussis. In this study, we compared AC-Hly toxins from various clinical isolates of B. pertussis and B. parapertussis, focusing on (i) the genomic sequences of cyaA genes, (ii) the PTMs of partially purified AC-Hly, and (iii) the cytotoxic activity of the various AC-Hly toxins. The genes encoding the AC-Hly toxins of B. pertussis and B. parapertussis displayed very limited polymorphism in each species. Most of the sequence differences between the two species were found in the C-terminal part of the protein. Both toxins harbored PTMs, mostly corresponding to palmitoylations of the lysine 860 residue and palmoylations and myristoylations of lysine 983 for B. pertussis and AC-Hly and palmitoylations of lysine 894 and myristoylations of lysine 1017 for B. parapertussis AC-Hly. Purified AC-Hly from B. pertussis was cytotoxic to macrophages, whereas that from B. parapertussis was not.

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Untitled

Cited by 25 publications

References 42 publications

Host Specificity of Ovine Bordetella parapertussis and the Role of Complement

Host Specificity of Ovine Bordetella parapertussis and the Role of Complement

Comparative genomics of the classical Bordetella subspecies: the evolution and exchange of virulence-associated diversity amongst closely related pathogens

Characterization of Post-Translational Modifications and Cytotoxic Properties of the Adenylate-Cyclase Hemolysin Produced by Various Bordetella pertussis and Bordetella parapertussis Isolates

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