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Rogers, Hilary J.; Bate, Neil; Combe, Jonathan P.; Sullivan, Jerome L.; Sweetman, Justin P.; Swan, Christo H.; Lonsdale, David; Twell, David

doi:10.1023/a:1010695226241

Cited by 175 publications

(32 citation statements)

References 20 publications

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“…Examination of the 5Ј-upstream region of Osg1 revealed the presence of several anther-and pollen-speciWc regulatory motifs, including Wve GTGA, two AGAAA, and one AGGTCA motifs. GTGA motifs have been identiWed in the promoter of the tobacco late pollen gene g10, and shown to be important in directing high levels of pollen-speciWc expression of the gene (Twell et al 1991;Rogers et al 2001). POLLEN1LELAT52 (AGAAA) is one of two codependent regulatory elements responsible for pollenspeciWc activation of the tomato LAT52 gene (Bate and Twell 1998;Filichkin et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…A search for cisacting elements in this putative Osg1 promoter (¡870 to +131) using plant cis-acting regulatory DNA elements (http://www.dna.affrc.go.jp/htdocs/PLACE/; Higo et al 1999) indicated that this putative promoter contained several anther/pollen-speciWc regulatory motifs. There are Wve copies of the "GTGA motif" (Rogers et al 2001), two copies of the POLLEN1LELAT52 motifs (AGAAA) (Bate and Twell 1998;Filichkin et al 2004), and a QELEM ENTZMZM13 motif (AGGTCA) (Hamilton et al 1998) (Fig. 1i).…”

Section: Analysis Of Osg1 Expression In Ricementioning

confidence: 99%

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A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

Wan

Zha

Cheng

et al. 2010

Planta

114

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Plant β-1,3-glucanases are involved in plant defense and development. In rice (Oryza sativa), 14 genes encoding putative β-1,3-glucanases have been isolated and sequenced. However, only limited information is available on the function of these β-1,3-glucanase genes. In this study, we report a detailed functional characterization of one of these genes, Osg1. Osg1 encodes a glucanase carrying no C-terminal extension. Osg1 was found to be expressed throughout the plant and highly expressed in florets, leaf sheaths, and leaf blades. Investigations using real-time PCR, immunocytochemical analysis, and a GUS-reporter gene driven by the Osg1 promoter indicated that Osg1 was mainly expressed at the late meiosis, early microspore, and middle microspore stages in the florets. To elucidate the role of Osg1, we suppressed expression of the Osg1 gene by RNA interference in transgenic rice. The silencing of Osg1 resulted in male sterility. The pollen mother cells appeared to be normal in Osg1-RI plants, but callose degradation was disrupted around the microspores in the anther locules of the Osg1-RI plants at the early microspore stage. Consequently, the release of the young microspores into the anther locules was delayed, and the microspores began to degenerate later. These results provide evidence that Osg1 is essential for timely callose degradation in the process of tetrad dissolution.

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Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Osg1 Expression In Ricementioning

confidence: 99%

A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

Wan

Zha

Cheng

et al. 2010

Planta

114

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“…Structural analysis of the Bet v 1a proximal promoter using network software (see Materials and Methods) identified putative CAAT and TATA boxes and other general regulatory elements modulating gene expression such as Box II [38]and GT-1 [39]. Furthermore, the Bet v 1a proximal promoter sequence contains elements responsible for expression in pollen, namely LAT52 [40], GTGA [41], and the enhancing Q (quantitative) element [42](fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…1). Originally, active GTGA elements were found in direct orientation within the tobacco g10 gene promoter [41]. Although the Bet v 1a promoter contains GTGA elements in the reverse orientation, it might not be essential for the pollen-specific induction since the GTGA element represents a type of Box II core sequence shown to act in an orientation-independent manner [57]as well as GT-1 elements [58].…”

Section: Discussionmentioning

confidence: 99%

A Model System to Study the Environment-Dependent Expression of the <i>Bet v 1a</i> Gene Encoding the Major Birch Pollen Allergen

Tashpulatov¹,

Clement²,

Akimcheva³

et al. 2004

Int Arch Allergy Immunol

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Background: The major birch pollen allergen Bet v 1 (or Bet v 1a) is one of the main causes of seasonal type I allergies. Various environmental factors such as light, temperature and air pollution may influence the activity of the Bet v 1a gene. The creation of a model system to evaluate the role of environmental factors affecting the Bet v 1a gene expression would be highly desirable. We suggest the use of transgenic tobacco plants carrying a Bet v 1a promoter-reporter gene fusion as such a system. Methods: The promoter of the Bet v 1a gene was isolated with the use of the Universal Genome Walker kit (BD Biosciences Clontech, USA). Web Software was used to search for putative cis-regulatory elements within the promoter. Transgenic tobacco plants harboring the promoter-β-glucuronidase (GUS) reporter gene fusion were obtained via Agrobacterium tumefaciens-mediated transformation. Promoter activity was examined with histochemical and quantitative assays. Results: Structural analysis predicted elements responsible for pollen-specific, light-, stress- and hormone-mediated induction within the Bet v 1a promoter. The evaluation of GUS activity in transgenic tobacco plants showed that the Bet v 1a promoter is pollen-specific. Moreover, the Bet v 1a promoter is considered to be the strongest isolated pollen-specific promoter reported to date. It was shown that temperature and abscisic acid positively regulate the activity of the Bet v 1a promoter during pollen development, providing evidence for environment-dependent regulation of the Bet v 1a gene. Conclusions: A model system to study the effect of environmental factors on the expression of the Bet v 1a gene encoding the major birch allergen in pollen was generated. Additionally, we suggest that this system could be used to search for factors that inhibit the activity of the gene in pollen in order to reduce the potential allergenicity of birch trees.

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“…No substitutions, insertions, or deletions within the isolated fragments were observed. All amplified fragments were analyzed to detect two known anther-specific elements, GTGANTG10 (Bate and Twell 1998) and POLLEN1LELAT (Rogers et al 2001), using PLACE Fig. 1 Expression profiles of anther-specific candidate genes.…”

Section: Isolation Of the Regulatory Regions Of Anther-specific Genesmentioning

confidence: 99%

Isolation of Anther-specific Gene Promoters Suitable for Transgene Expression in Rice

et al. 2010

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To isolate rice anther-specific promoters that allow strong and constitutive expression of transgenes under chilling stress conditions, a genome-wide screen using a microarray was conducted. Six candidate genes for anther-specific expression were selected after comparison of expression levels in leaf and anther tissues from booting stage plants. These genes encoded a mandelonitrile lyase (OsACE1), a sterol desaturase (OsCER1), a MADS-box protein (OsMADS58), a polygalacturonase (OsPGT1), an ERF-like AP2 domain-containing protein (OsERFL1), and a ribosome-inactivating protein-like gene (OsRIP1). Reverse transcriptase-polymerase chain reaction analyses confirmed anther-specific expression of these genes under controlled and in-field chilling conditions. Promoter regions of the selected genes were isolated and fused to the β-glucuronidase (GUS) reporter gene, and the constructs were introduced into rice plants. GUS expression in all transgenic plants except for those carrying OsERFL1and OsRIP1 constructs was only detectable in anthers within the panicles, and no GUS activity was detected in leaves.However, OsERFL1 and OsRIP1 promoters were shown to drive GUS expression in paleae and glumes as well as anthers. GUS expression levels of four transgenic plants were also tested with 2-week-old seedlings and grains. Promoters for OsACE1, OsCER1, OsMADS58, and OsPGT1 were shown to be silent in roots and aerial parts of the seedlings and mature grains. Therefore, these four promoters were deemed suitable for anther-specific transgene expression in rice.

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Cited by 175 publications

References 20 publications

A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

A rice β-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

A Model System to Study the Environment-Dependent Expression of the <i>Bet v 1a</i> Gene Encoding the Major Birch Pollen Allergen

Isolation of Anther-specific Gene Promoters Suitable for Transgene Expression in Rice

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