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Leclerc, Éric; Sakai, Yasuyuki; Fujii, Teruo

doi:10.1023/a:1024583026925

Cited by 291 publications

(139 citation statements)

References 9 publications

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“…PDMS has become the preferred material for biomedical electrons and microscale fluid devices due to the following advantages: (a) low production cost compared to traditional MEMS substrate materials such as silicon or glass; (b) optical transparency (transparent for a wavelength range of 400-700 nm); (c) biocompatibility; and (d) easy bonding to itself or other substrates. [10][11][12][13][14] In addition to those advantages, the rubber elastic properties of PDMS have become versatile tools for microfluidic devices and have been used in micro-flow cytometry, peristaltic pumping and mixing, microvalves, and portable immunosensing systems. [15][16][17][18] A highly flexible PDMS chip also was used to maintain chemostatic conditions for bacterial and yeast colony growth.…”

Section: Introductionmentioning

confidence: 99%

Deformation properties between fluid and periodic circular obstacles in polydimethylsiloxane microchannels: Experimental and numerical investigations under various conditions

2013

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Understanding the mechanical properties of optically transparent polydimethylsiloxane (PDMS) microchannels was essential to the design of polymer-based microdevices. In this experiment, PDMS microchannels were filled with a 100 lM solution of rhodamine 6G dye at very low Reynolds numbers ($10 À3 ). The deformation of PDMS microchannels created by pressure-driven flow was investigated by fluorescence microscopy and quantified the deformation by the linear relationship between dye layer thickness and intensity. A line scan across the channel determined the microchannel deformation at several channel positions. Scaling analysis widely used to justify PDMS bulging approximation was allowed when the applied flow rate was as high as 2.0 ll/min. The three physical parameters (i.e., flow rate, PDMS wall thickness, and mixing ratio) and the design parameter (i.e., channel aspect ratio ¼ channel height/channel width) were considered as critical parameters and provided the different features of pressure distributions within polymer-based microchannel devices. The investigations of the four parameters performed on flexible materials were carried out by comparison of experiment and finite element method (FEM) results. The measured Young's modulus from PDMS tensile test specimens at various circumstances provided reliable results for the finite element method. A thin channel wall, less cross-linker, high flow rate, and low aspect ratio microchannel were inclined to have a significant PDMS bulging. Among them, various mixing ratios related to material property and aspect ratios were one of the significant factors to determine PDMS bulging properties. The measured deformations were larger than the numerical simulation but were within corresponding values predicted by the finite element method in most cases. V C 2013 AIP Publishing LLC. [http://dx

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Section: Introductionmentioning

confidence: 99%

Deformation properties between fluid and periodic circular obstacles in polydimethylsiloxane microchannels: Experimental and numerical investigations under various conditions

2013

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“…pump ͉ valve ͉ bioreactor ͉ mixer ͉ perfusion A dvanced microfluidic cellular assays (1)(2)(3)(4)(5)(6) and microscale tissue engineering studies (7)(8)(9)(10) would benefit from robust and convenient methods to computer-control accurate spatiotemporal patterns of microfluidic flows in arrays of fluidic networks. In the past, fluidic control included syringe pumps (11), hydrogel valves (12), gravity-driven pumps (13), evaporation-based pumps (14,15), acoustic pumps (16), gas-generationbased pumps (17,18), and centrifugal force in CD chips (19).…”

mentioning

confidence: 99%

Computerized microfluidic cell culture using elastomeric channels and Braille displays

Zhu

Futai

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

365

312

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Computer-controlled microfluidics would advance many types of cellular assays and microscale tissue engineering studies wherever spatiotemporal changes in fluidics need to be defined. However, this goal has been elusive because of the limited availability of integrated, programmable pumps and valves. This paper demonstrates how a refreshable Braille display, with its grid of 320 vertically moving pins, can power integrated pumps and valves through localized deformations of channel networks within elastic silicone rubber. The resulting computerized fluidic control is able to switch among: (i) rapid and efficient mixing between streams, (ii) multiple laminar flows with minimal mixing between streams, and (iii) segmented plug-flow of immiscible fluids within the same channel architecture. The same control method is used to precisely seed cells, compartmentalize them into distinct subpopulations through channel reconfiguration, and culture each cell subpopulation for up to 3 weeks under perfusion. These reliable microscale cell cultures showed gradients of cellular behavior from C2C12 myoblasts along channel lengths, as well as differences in cell density of undifferentiated myoblasts and differentiation patterns, both programmable through different flow rates of serumcontaining media. This technology will allow future microscale tissue or cell studies to be more accessible, especially for highthroughput, complex, and long-term experiments. The microfluidic actuation method described is versatile and computer programmable, yet simple, well packaged, and portable enough for personal use.pump ͉ valve ͉ bioreactor ͉ mixer ͉ perfusion A dvanced microfluidic cellular assays (1-6) and microscale tissue engineering studies (7-10) would benefit from robust and convenient methods to computer-control accurate spatiotemporal patterns of microfluidic flows in arrays of fluidic networks. In the past, fluidic control included syringe pumps (11), hydrogel valves (12), gravity-driven pumps (13), evaporation-based pumps (14, 15), acoustic pumps (16), gas-generationbased pumps (17, 18), and centrifugal force in CD chips (19). Electrokinetic flow (20-22), thermopneumatic (23, 24), pneumatic͞hydraulic (25), or mechanical (26, 27) valves and pumps have a high degree of control, but require external connection lines to larger equipment for actuation. A few fully integrated and self-packaged systems (23, 28) have been developed, but lack the reconfigurability inherent with numerous active valves and pumps.Here, we report a method to precisely control fluid flow inside elastomeric capillary networks by using multiple (tens to hundreds) computer-controlled, piezoelectric, movable pins. These pins are positioned as a grid on a refreshable Braille display (e.g., F. H. Papenmeier, Schwerte, Germany), which is a tactile device used by the visually impaired to read computer text. Each pin can act as a valve and be shifted upward to push against channels contained in silicone rubber and completely shut the channel. Three sequential valves of this...

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“…[31] The albumin production rate was measured to be 24.3±5.5 µg cm −2 day −1 , which was comparable with previous reports. [10,12] A maximum albumin production rate of 30.5±0.2 µg day −1 per device occurred during the first 24 h, which can be attributed to the albumin production of cells located in the waste container that did not attach during the seeding. Reactors were perfused for a period of up to one week to demonstrate long-term viability within the devices.…”

mentioning

confidence: 99%

Three‐Dimensional Microfluidic Tissue‐Engineering Scaffolds Using a Flexible Biodegradable Polymer

et al. 2006

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Untitled

Cited by 291 publications

References 9 publications

Deformation properties between fluid and periodic circular obstacles in polydimethylsiloxane microchannels: Experimental and numerical investigations under various conditions

Deformation properties between fluid and periodic circular obstacles in polydimethylsiloxane microchannels: Experimental and numerical investigations under various conditions

Computerized microfluidic cell culture using elastomeric channels and Braille displays

Three‐Dimensional Microfluidic Tissue‐Engineering Scaffolds Using a Flexible Biodegradable Polymer

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