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Abstract

We have previously reported the participation of the protein kinase CK2 in the mechanism by which salicylic acid activates transcription of genes, such as those coding for glutathion S-transferases, in tobacco. With the purpose of further studying the participation of CK2 in this signal transduction pathway, we isolated and sequenced the cDNA from the NtCK2A gene, coding for the catalytic alpha subunit of CK2 from tobacco. The NtCK2A cDNA was isolated by screening of a tobacco cDNA library with a heterologous probe from Arabidopsis thaliana, followed by 3' RACE to obtain the 3' region. Sequence analysis of the NtCK2A cDNA showed a high level of identity between this CK2alpha protein sequence and the corresponding sequences of other plant species such as Arabidopsis and maize (92-95% identity), or those of animal species such as human and Xenopus laevis (75% identity). The expression of the NtCK2A gene in different tissues from tobacco plants was analyzed by Northern blot. High levels of expression of this gene were observed in proliferating tissues such as shoot and root apical meristems. A recombinant CK2alpha protein was obtained after expression of the NtCK2A cDNA in Escherichia coli. The ability of this recombinant CK2alpha subunit to phosphorylate casein was inhibited by heparin and stimulated by the CK2beta subunit from Xenopus laevis.

Phosphorylation of TGB1 by protein kinase CK2 promotes barley stripe mosaic virus movement in monocots and dicots

“…Primers for amplifying NbCK2α were based on the flanking sequence of the N. tabacum CK2α ORF (GenBank accession no. AF374474) ( Salinas et al , 2001 ). The PCR products of the NbCK2α and HvCK2α genes were inserted into the Nde I/ Xho I, and Nde I/ Sal I sites of pET-30a(+), respectively, to generate pET-NbCK2α and pET-HvCK2α.…”

High-Throughput Sequencing Reveals Novel microRNAs Involved in the Continuous Flowering Trait of Longan (Dimocarpus longan Lour.)

“…Furthermore, the presence of CK2 is essential for regulating circadian rhythms, hormone responses, light signaling, and flowering time control [ 57 , 58 ]. The CK2 protein has an evolutionarily conserved role as a component of the circadian clock in a variety of organisms, including diverse plant species, wheat, rice, tobacco, maize, mustard, and Arabidopsis [ 55 , 59 , 60 ]. In this study, the CK2 gene was DEmRNA in the integrated analysis of the transcriptome.…”