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Meng, Jianghong; Shi, Suwen; Gan, Li; Li, Zaiyung; Xin-shun, Qu

doi:10.1023/a:1018646223643

Cited by 102 publications

(18 citation statements)

References 5 publications

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“…While it is now clear that the B genome shares some regions of homology with the A and C genomes (Lagercrantz and Lydiate 1996;Panjabi et al 2008), the Brassica Bgenome chromosomes do not pair with chromosomes of the A and C genomes in interspecific crosses (Meng et al 1998). The B genome has significantly diverged from the A and C genomes (Axelsson et al 2000;Warwick et al 1992), as evident from cytological observations of preferential pairing between homologous chromosomes in digenomic triploids (BBC and CCB) generated from interspecific hybridization between B. carinata and B. nigra and B. carinata and B. oleracea (Attia et al 1987).…”

Section: Discussionmentioning

confidence: 99%

Introgression of B-genome chromosomes in a doubled haploid population ofBrassica napus ×B. carinata

Navabi

Parkin

Pires

et al. 2010

Genome

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The Brassica B-genome species possess many valuable agronomic and disease resistance traits. To transfer traits from the B genome of B. carinata into B. napus, an interspecific cross between B. napus and B. carinata was performed and a doubled haploid (DH) population was generated from the BC2S3 generation. Successful production of interspecific DH lines as identified using B-genome microsatellite markers is reported. Five percent of DH lines carry either intact B-genome chromosomes or chromosomes that have deletions. All of the DH lines have linkage group J13/B7 in common. This was further confirmed using B. nigra genomic DNA in a fluorescent in situ hybridization assay where the B-genome chromosomes were visualized and distinguished from the A- and C-genome chromosomes. The 60 DH lines were also evaluated for morphological traits in the field for two seasons and were tested for resistance to blackleg, caused by Leptosphaeria maculans, under greenhouse conditions. Variation in the DH population followed a normal distribution for several agronomic traits and response to blackleg. The lines with B-genome chromosomes were significantly different (p < 0.01) from the lines without B-genome chromosomes for both morphological and seed quality traits such as days to flowering, days to maturity, and erucic acid content.

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Section: Discussionmentioning

confidence: 99%

Introgression of B-genome chromosomes in a doubled haploid population ofBrassica napus ×B. carinata

Navabi

Parkin

Pires

et al. 2010

Genome

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“…It consists of the two homologous genomes, B and C, and may have originated as an allotetraploid species as a result of spontaneous hybridization between diploid species; Brassica nigra (2 n = 2 x = 14, genome BB) and Brassica oleracea (2 n = 2 x = 18, genome CC) in Ethiopia (Nagaharu, 1935; Lukens et al, 2004; Warwick, 2011). B. carinata harbors useful genes for resistance to abiotic and biotic stresses (Getinet et al, 1996), and therefore has been used as a donor for introgression of genes to improve and widen the gene pool of Brassica rapa, Brassica napus , and Brassica juncea germplasm (Meng et al, 1998; Xiao et al, 2010; Wei et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Investigation of the Genetic Diversity and Quantitative Trait Loci Accounting for Important Agronomic and Seed Quality Traits in Brassica carinata

Zhang

Raman

et al. 2017

Front. Plant Sci.

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Brassica carinata (BBCC) is an allotetraploid in Brassicas with unique alleles for agronomic traits and has huge potential as source for biodiesel production. To investigate the genome-wide molecular diversity, population structure and linkage disequilibrium (LD) pattern in this species, we genotyped a panel of 81 accessions of B. carinata with genotyping by sequencing approach DArTseq, generating a total of 54,510 polymorphic markers. Two subpopulations were exhibited in the B. carinata accessions. The average distance of LD decay (r2 = 0.1) in B subgenome (0.25 Mb) was shorter than that of C subgenome (0.40 Mb). Genome-wide association analysis (GWAS) identified a total of seven markers significantly associated with five seed quality traits in two experiments. To further identify the quantitative trait loci (QTL) for important agronomic and seed quality traits, we phenotyped a doubled haploid (DH) mapping population derived from the “YW” cross between two parents (Y-BcDH64 and W-BcDH76) representing from the two subpopulations. The YW DH population and its parents were grown in three contrasting environments; spring (Hezheng and Xining, China), semi-winter (Wuhan, China), and spring (Wagga Wagga, Australia) across 5 years for QTL mapping. Genetic bases of phenotypic variation in seed yield and its seven related traits, and six seed quality traits were determined. A total of 282 consensus QTL accounting for these traits were identified including nine major QTL for flowering time, oleic acid, linolenic acid, pod number of main inflorescence, and seed weight. Of these, 109 and 134 QTL were specific to spring and semi-winter environment, respectively, while 39 consensus QTL were identified in both contrasting environments. Two QTL identified for linolenic acid (B3) and erucic acid (C7) were validated in the diverse lines used for GWAS. A total of 25 QTL accounting for flowering time, erucic acid, and oleic acid were aligned to the homologous QTL or candidate gene regions in the C genome of B. napus. These results would not only provide insights for genetic improvement of this species, but will also identify useful genetic variation hidden in the Cc subgenome of B. carinata to improve canola cultivars.

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“…Rapeseed use is limited by the concentration of anti-nutritional factors, including phenolic compounds, lignin, tannins, and proanthocyanidins, it contains. Previous research showed that yellow-seeded B. napus has a thinner seed coat, less pigmentation, and higher protein and oil contents than does black-seeded B. napus in the same background, rendering it a more nutritional feed for livestock (Chen and Heneen, 1992; Tang et al, 1997; Meng et al, 1998). Thus, selecting lines with a stable yellow-seed trait is one of the most important breeding aims for B. napus .…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Survey of Flavonoid Biosynthesis Genes and Gene Expression Analysis between Black- and Yellow-Seeded Brassica napus

Zhao

et al. 2016

Front. Plant Sci.

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Flavonoids, the compounds that impart color to fruits, flowers, and seeds, are the most widespread secondary metabolites in plants. However, a systematic analysis of these loci has not been performed in Brassicaceae. In this study, we isolated 649 nucleotide sequences related to flavonoid biosynthesis, i.e., the Transparent Testa (TT) genes, and their associated amino acid sequences in 17 Brassicaceae species, grouped into Arabidopsis or Brassicaceae subgroups. Moreover, 36 copies of 21 genes of the flavonoid biosynthesis pathway were identified in Arabidopsis thaliana, 53 were identified in Brassica rapa, 50 in Brassica oleracea, and 95 in B. napus, followed the genomic distribution, collinearity analysis and genes triplication of them among Brassicaceae species. The results showed that the extensive gene loss, whole genome triplication, and diploidization that occurred after divergence from the common ancestor. Using qRT-PCR methods, we analyzed the expression of 18 flavonoid biosynthesis genes in 6 yellow- and black-seeded B. napus inbred lines with different genetic background, found that 12 of which were preferentially expressed during seed development, whereas the remaining genes were expressed in all B. napus tissues examined. Moreover, 14 of these genes showed significant differences in expression level during seed development, and all but four of these (i.e., BnTT5, BnTT7, BnTT10, and BnTTG1) had similar expression patterns among the yellow- and black-seeded B. napus. Results showed that the structural genes (BnTT3, BnTT18, and BnBAN), regulatory genes (BnTTG2 and BnTT16) and three encoding transfer proteins (BnTT12, BnTT19, and BnAHA10) might play an crucial roles in the formation of different seed coat colors in B. napus. These data will be helpful for illustrating the molecular mechanisms of flavonoid biosynthesis in Brassicaceae species.

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Cited by 102 publications

References 5 publications

Introgression of B-genome chromosomes in a doubled haploid population ofBrassica napus ×B. carinata

Introgression of B-genome chromosomes in a doubled haploid population ofBrassica napus ×B. carinata

Investigation of the Genetic Diversity and Quantitative Trait Loci Accounting for Important Agronomic and Seed Quality Traits in Brassica carinata

Genome-Wide Survey of Flavonoid Biosynthesis Genes and Gene Expression Analysis between Black- and Yellow-Seeded Brassica napus

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