Z. Platková, M. Polakovič, V. Štefuca, M. Vandáková, and M. Antošová: Selection of Carrier for Immobilization of Fructosyltransferase from Aureobasidium pullulans

Onderková, Z.; Polakovič, Milan; Štefuca, Vladimı́r; Vandáková, M.; Antošová, Monika

doi:10.2478/s11696-007-0013-8

Cited by 2 publications

(5 citation statements)

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“…A stabilisation effect of about 2 °C was observed for the inactivation of pectin methylesterase in different fruit juices containing about 10-15 % of saccharides (Wilińska et al, 2008). A much stronger stabilisation effect was observed for fructosyltransferase inactivation in a 700 g/L saccharide solution (Onderková et al, 2010). It was estimated to be from 8 °C at higher temperatures to 15 °C at lower temperatures.…”

Section: Resultsmentioning

confidence: 93%

“…It had to be fi xed to get good statistical charac teristics of the remaining model para meters. This is a common problem for models based on the Lumry-Eyring mechanism (Polakovič and Bryjak, 2002;Onderková et al, 2010). The estimated activation energies E 1 and E 2 can be compared with the values presented by other authors.…”

Section: Resultsmentioning

confidence: 95%

“…In this study, a kinetic model (Onderková et al, 2010) was examined for the description of the courses of relative enzyme activity, a, defi ned as the ratio of c N , the concentration of native enzyme form, N, in time, t, and c N0 , its initial concentration. This model was based on the mechanism:…”

Section: Mathematical Modellingmentioning

confidence: 99%

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Thermal inactivation kinetics of Aspergillus oryzae β-galactosidase in concentrated lactose solution

Polakovič

2018

Acta Chimica Slovaca

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Thermal inactivation of a commercial β-galactosidase from Aspergillus oryzae in a 300 g/L lactose solution was studied in the temperature range of 65-75 °C. Lactose exhibited a stabilisation effect when similar inactivation rates as those in lactose solution were observed in a lactose-free solution at temperatures lower by 5 °C. Inactivation process in the lactose solution was biphasic. A kinetic model based on the Lumry-Eyring mechanism was proposed and successfully verifi ed. Estimated activation energy values were very different. Rather high activation energy values of the forward reactions were responsible for both the signifi cant change of rate constants and the rate-controlling reaction with temperature. For these two reasons, an increase of the operational lifetime of the enzyme from 7 days at 60 °C to 580 days at 55 °C was predicted.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 95%

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Thermal inactivation kinetics of Aspergillus oryzae β-galactosidase in concentrated lactose solution

Polakovič

2018

Acta Chimica Slovaca

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“…These different observations indicate that the U t (U/volume) change during cultivation was affected by cell concentrations and U t (U/mg cells). Moreover, since the transfructosylating enzymes were likely composed of mixed enzymes (Salinas and Perotti 2009 ; Shin et al 2004 ; Vandáková et al 2004 ; Xie et al 2017 ; Yoshikawa et al 2006 ; Zhang et al 2016 ), measured U t (U/mg cells) was likely the overall activity of total enzymes rather than individual enzymes.…”

Section: Resultsmentioning

confidence: 99%

“…The synthetic molasses medium contained total nitrogen and phosphorous concentrations of 2.57 g/L (with addition of 10.0 g/L NaNO 3 and 8.5 g/L yeast extract) and 0.88 g/L phosphorus (with addition of 5.0 g/L K 2 HPO 4 ), respectively, as per the molasses medium. The synthetic molasses medium also contained 0.5 g/L MgSO 4 , which was a commonly used concentration (Dominguez et al 2012 ; Shin et al 2004 ; Vandáková et al 2004 ). Aliquots (45 mL) of molasses and synthetic molasses media were transferred into 250-mL conical flasks and autoclaved at 121 °C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A

Khatun

Hassanpour

Mussatto

et al. 2021

Bioresour. Bioprocess.

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Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A. pullulans. A. pullulans in molasses-based medium produced cells and broth with a total transfructosylating activity of 123.6 U/mL compared to 61.0 and 85.8 U/mL in synthetic molasses-based and sucrose-based media, respectively. It was found that inclusion of glucose in sucrose medium reduced both transfructosylating and hydrolytic activities of the produced cells and broth. With the use of pure glucose medium, cells and broth had very low levels of transfructosylating activities and hydrolytic activities were not detected. These results indicated that A. pullulans FRR 5284 produced both constitutive and inducible enzymes in sucrose-rich media, such as molasses while it only produced constitutive enzymes in the glucose media. Furthermore, treatment of FOS solutions generated from sucrose-rich solutions using an invertase-deficient Saccharomyces yeast converted glucose to ethanol and acetic acid and improved FOS content in total sugars by 20–30%. Treated FOS derived from molasses improved the in vitro growth of nine probiotic strains by 9–63% compared to a commercial FOS in 12 h incubation. This study demonstrated the potential of using molasses to produce FOS for feed application.

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Z. Platková, M. Polakovič, V. Štefuca, M. Vandáková, and M. Antošová: Selection of Carrier for Immobilization of Fructosyltransferase from Aureobasidium pullulans

Cited by 2 publications

References 1 publication

Thermal inactivation kinetics of Aspergillus oryzae β-galactosidase in concentrated lactose solution

Thermal inactivation kinetics of Aspergillus oryzae β-galactosidase in concentrated lactose solution

Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A

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