“…YY1 (Yin-Yang-1) is a ubiquitously expressed transcription factor that activates or represses speci c genes to in uence cellular regulatory mechanisms, including cell growth control, apoptosis, and pregnancy regulation [56][57]. The YY1 gene is reportedly implicated in the regulation of human uterine artery endothelial cell development, cytoskeleton formation, and trophoblast cell invasion and adhesion [25][26][27][28]. In addition, YY1 is involved in early uterine metaphase and embryo implantation in mice through the regulation of Rtcb promoter activity [30], and YY1 mutations are associated with embryonic lethality in mice [58].…”
Section: Discussionmentioning
confidence: 99%
“…MMP2 [26], ITGB3/7 [27], PVT1 [28], and RTCB [29]. Additionally, protein phase-work network analysis revealed that CSK proteins showed direct interactions with several proteins (Fig.…”
Section: Screening Of Embryo Attachment-related Target Genesmentioning
MicroRNAs (miRNAs)-a class of small endogenous non-coding RNAs-are widely involved in posttranscriptional gene regulation of numerous physiological processes. High-throughput sequencing revealed that the miR-192 expression level appeared to be signi cantly higher in the blood exosomes of sows at early gestation than that in non-pregnant sows. Furthermore, miR-192 was hypothesized to have a regulatory role in embryo implantation; however, the target genes involved in exerting the regulatory function of miR-192 required further elucidation.Methods: In the present study, potential target genes of miR-192 in porcine endometrial epithelial cells (PEECs) were identi ed through biotin-labeled miRNA pull-down; functional and pathway enrichment analysis was performed via gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment. Bioinformatic analyses were concurrently used to predict the potential target genes associated with sow embryo implantation. In addition, double luciferase reporter vectors, reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), and Western blot were performed to verify the targeting and regulatory roles of the abovementioned target genes.Results: A total of 1688 differentially expressed mRNAs were identi ed via miRNA pull-down. Through RT-qPCR, the accuracy of the sequencing data was veri ed. In the bioinformatics analysis, potential target genes of miR-192 appeared to form a dense inter-regulatory network and regulated multiple signaling pathways, such as metabolic pathways and the PI3K-Akt, MAPKs, and mTOR signaling pathways, that are relevant to the mammalian embryo implantation process. In addition, CSK (C-terminal Src kinase) and YY1 (Yin-Yang-1) were predicted to be potential candidates, and we validated that miR-192 directly targets and suppresses the expression of the CSK and YY1 genes.Conclusion: We screened 1688 potential target genes of miR-192 were screened, and CSK and YY1 were identi ed as miR-192 target genes. The outcomes of the present study provide novel insights into the regulatory mechanism of porcine embryo implantation and the identi cation of miRNA target genes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
