Yunpi Heluo decoction attenuates insulin resistance by regulating liver miR-29a-3p in Zucker diabetic fatty rats

Mao, Zhu-Jun; Weng, Siying; Lin, Min; Ke-fu, Chai

doi:10.1016/j.jep.2019.111966

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…Therefore, together with miR-34a-5p, miR-20a-3p can participate in the ONC-elicited growth inhibition, and it can counteract the cancer metabolic phenotype as well. In addition, in silico analysis of the predicted targets (Figure 5) demonstrated that PDK1, HIF1α, and MAPK1 can be targets of miR-29a-3p, suggesting a possible role of miR-29a-3p in metabolism, in agreement with its role in insulin receptor signaling in the liver of diabetic rats [73].…”

Section: Discussionmentioning

confidence: 76%

Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins

Tomi

Campagnari

et al. 2022

IJMS

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Onconase (ONC) is an amphibian secretory ribonuclease displaying cytostatic and cytotoxic activities against many mammalian tumors, including melanoma. ONC principally damages tRNA species, but also other non-coding RNAs, although its precise targets are not known. We investigated the ONC ability to modulate the expression of 16 onco-suppressor microRNAs (miRNAs) in the A375 BRAF-mutated melanoma cell line. RT-PCR and immunoblots were used to measure the expression levels of miRNAs and their regulated proteins, respectively. In silico study was carried out to verify the relations between miRNAs and their mRNA targets. A375 cell transfection with miR-20a-3p and miR-34a-5p mimics or inhibitors was performed. The onco-suppressors miR-20a-3p, miR-29a-3p and miR-34a-5p were highly expressed in 48-h ONC-treated A375 cells. The cytostatic effect of ONC in A375 cells was mechanistically explained by the sharp inhibition of cyclins D1 and A2 expression level, as well as by downregulation of retinoblastoma protein and cyclin-dependent-kinase-2 activities. Remarkably, the expression of kinases ERK1/2 and Akt, as well as of the hypoxia inducible factor-1α, was inhibited by ONC. All these proteins control pro-survival pathways. Finally, many crucial proteins involved in migration, invasion and metastatic potential were downregulated by ONC. Results obtained from transfection of miR-20a-3p and miR-34a-5p inhibitors in the presence of ONC show that these miRNAs may participate in the antitumor effects of ONC in the A375 cell line. In conclusion, we identified many intracellular downregulated proteins involved in melanoma cell proliferation, metabolism and progression. All mRNAs coding these proteins may be targets of miR-20a-3p, miR-29a-3p and/or miR-34a-5p, which are in turn upregulated by ONC. Data suggest that several known ONC anti-proliferative and anti-metastatic activities in A375 melanoma cells might depend on the upregulation of onco-suppressor miRNAs. Notably, miRNAs stability depends on the upstream regulation by long-non-coding-RNAs or circular-RNAs that can, in turn, be damaged by ONC ribonucleolytic activity.

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Section: Discussionmentioning

confidence: 76%

Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins

Tomi

Campagnari

et al. 2022

IJMS

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“…A study using human coronary artery endothelial cell-derived exosomes found that chrysin treatment reduced endothelial cellderived miR-92a-containing exosomes which then caused an increase in Klf2 expression for an atheroprotective effect (Lin et al, 2021). Yunpi Heluo decoction improved insulin resistance in Zucker diabetic fatty rats by reducing miR-29a-3p expression causing increased Irs1 expression, a target of miR-29a-3p (Mao et al, 2019), thereby increasing the protein expression of IRS1, protein kinase B, and pyruvate dehydrogenase lipoamide kinase isozyme 1 (PDK1) (Mao et al, 2019). Another study found that quercetin, perillyl alcohol, and berberine improved pathological conditions in pulmonary arterial hypertension by regulating miR-204 and miR-27a, as well as factors that play a role in inflammation, fibrosis, and apoptosis (Rajabi et al, 2021).…”

Section: Botanical Drugs That Modulate Ncrnamentioning

confidence: 99%

The efficacy of botanical drugs in orchestrating epigenetic modifications for ameliorating metabolic disorders

Ariyanto

2024

Front. Pharmacol.

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“…Apart from that, exosomes can also regulate insulin sensitivity by directly acting on the insulin receptor. For example, adipose-derived exosomes miR-29a-3p in the Zucker diabetic rat significantly aggravates IR in the liver by downregulating the expression of insulin receptor substrate-1 and its phosphorylated levels [64].…”

Section: Irmentioning

confidence: 99%

Exosomes: New Insights into the Pathogenesis of Metabolic Syndrome

Wang,

Li,

et al. 2023

Biology

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Exosomes are a subtype of extracellular vesicles (EVs) with a diameter of 30~150 nm (averaging ~100 nm) that are primarily produced through the endosomal pathway, and carry various components such as lipids, proteins, RNA, and other small molecular substances. Exosomes can mediate intercellular communication through the bioactive substances they carry, thus participating in different physiological activities. Metabolic syndrome (MS) is a disease caused by disturbances in the body’s metabolism, mainly including insulin resistance (IR), diabetes, obesity, non-alcoholic fatty liver disease (NAFLD), hyperlipidemia, and atherosclerosis (AS). Recent studies have shown that exosomes are closely related to the occurrence and development of MS. Exosomes can act as messengers to mediate signaling transductions between metabolic cells in the organism and play a bidirectional regulatory role in the MS process. This paper mainly reviews the components, biogenesis, biological functions and potential applications of exosomes, and exosomes involved in the pathogenesis of MS as well as their clinical significance in MS diagnosis.

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Yunpi Heluo decoction attenuates insulin resistance by regulating liver miR-29a-3p in Zucker diabetic fatty rats

Cited by 6 publications

References 30 publications

Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins

Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins

The efficacy of botanical drugs in orchestrating epigenetic modifications for ameliorating metabolic disorders

Exosomes: New Insights into the Pathogenesis of Metabolic Syndrome

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