“…Protein concentrations were analyzed using the BCA Protein Assay Kit (Thermo Fisher Scientific, Inc.), and 30 μg protein was loaded in each lane for gel electrophoresis. The procedure were performed as previously described 21 . The primary antibodies used were as follows: anti-COX-2 (1:1000), anti-CD36 (1:1000), anti-phospho-p38 (1:1000), anti-p38 (1:2000), anti-phospho-JNK (1:2000), anti-JNK (1:2000), anti-phospho-ERK1/2 (1:2000), anti-ERK1/2 (1:1000), anti-STAR (1:1000), anti-p450scc (1:1000), anti-3β-HSD (1:2000), anti-17β-HSD (1:200), anti-Ndufs1 (1:2000), anti-SdhB (1:10,000), anti-Uqcrfs1 (1:4000), anti-Fech (1:1000), anti-CytC (1:1000), and GAPDH (1:2000).…”