You Can’t Go Home Again: Transcriptionally Driven Alteration of Cell Signaling by NGF

Abstract

Here we review findings indicating that neurotrophins such as NGF promote changes in gene transcription that in turn influence the ways that cells subsequently respond to trophic factors. As a result, initial responses of "naïve" cells to NGF and other trophic agents differ from those of cells with prior NGF exposure. We discuss specific examples based on reports in the literature as well as on data derived from a serial analysis of gene expression (SAGE) study of NGF-promoted transcriptional changes in PC12 pheochromocytoma cells.

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

“…Many of the known genes and proteins regulated by PACAP are associated with neuritogenesis [i.e., DISC1-binding zinc-finger protein or early growth response 1 Ravni et al, 2008)], hormone secretion [i.e., selenoprotein T (Grumolato et al, 2003a(Grumolato et al, , 2008], cell growth [i.e., growth arrest specific 1 or cyclin B2 (Vaudry et al, 2002b)], and cell survival [i.e., caspase3 or serum/glucocorticoid regulated kinase (Lebon et al, 2006;Ravni et al, 2006aRavni et al, , 2008Samal et al, 2007)]. It should be pointed out, however, that during differentiation, PACAP probably synergizes with other growth factors to induce the full functional phenotype of neuroendocrine cells (Lazarovici and Fink, 1999;Hashimoto et al, 2000a;Sakai et al, 2001;Vaudry and Taupenot, 2002;Beaujean et al, 2003;Greene and Angelastro, 2005).…”

Bactenecin 7 peptide fragment as a tool for intracellular delivery of a phosphorescent oxygen sensor

“…As the PEPP0 conjugate has an improved intracellular distribution relative to that of the PtCPTE-CFR9 probe (with its closer proximity to mitochondria), this probe represents a more versatile tool for the monitoring of the intracellular O 2 concentration. Differentiated PC12 cells are widely used in the study of neurosecretion, neuroprotection and Alzheimer's disease [32][33][34][35][36]. These cells are a viable model for respirometric experiments because of their active respiration profile, analogous to cerebellar granular neurones [22,37].…”

The Development of Developmental Neuroscience

“…In vivo, the target supplies neurotrophins, which activate Trk receptors on axons (Patapoutian and Reichardt, 2001). In the late 80s and 90s, other neurotrophins such as NT3, NT4, and BDNF were identified, along with their receptors and coreceptors, and downstream signaling and transcriptional events were highlighted (Greene and Angelastro, 2005). Most compelling was the finding that growth factor receptors, such as p75, can act either in survival or death pathways (Haase et al, 2008).…”