YOLOv5-FPN: A Robust Framework for Multi-Sized Cell Counting in Fluorescence Images

Abstract

Cell counting in fluorescence microscopy is an essential task in biomedical research for analyzing cellular dynamics and studying disease progression. Traditional methods for cell counting involve manual counting or threshold-based segmentation, which are time-consuming and prone to human error. Recently, deep learning-based object detection methods have shown promising results in automating cell counting tasks. However, the existing methods mainly focus on segmentation-based techniques that require a large amount of labeled data and extensive computational resources. In this paper, we propose a novel approach to detect and count multiple-size cells in a fluorescence image slide using You Only Look Once version 5 (YOLOv5) with a feature pyramid network (FPN). Our proposed method can efficiently detect multiple cells with different sizes in a single image, eliminating the need for pixel-level segmentation. We show that our method outperforms state-of-the-art segmentation-based approaches in terms of accuracy and computational efficiency. The experimental results on publicly available datasets demonstrate that our proposed approach achieves an average precision of 0.8 and a processing time of 43.9 ms per image. Our approach addresses the research gap in the literature by providing a more efficient and accurate method for cell counting in fluorescence microscopy that requires less computational resources and labeled data.

Unbiased Complete Estimation of Chloroplast Number in Plant Cells Using Deep Learning Methods

“…In YOLO, a single neural network is applied to the entire image, dividing it into a grid. This enables YOLO to make real-time predictions with impressive speed, as it only needs a single pass through the network to detect objects in an image, making it an excellent choice for cell detecting and counting (Redmon et al, 2016a;Redmon and Farhadi, 2016b;Alam and Islam,2019;Aldughayfiq et al, 2023). However, to the best of our knowledge, there have been no applications of this method to quantify subcellular structures such as chloroplasts or mitochondria using 3D images obtained by optical microscopes from living cells.…”