Yolk hormones influence in ovo chemosensory learning, growth, and feeding behavior in domestic chicks

Abstract

In this study, we assessed whether prenatal exposure to elevated yolk steroid hormones can influence in ovo chemosensory learning and the behavior of domestic chicks. We simulated a maternal environmental challenge by experimentally enhancing yolk progesterone, testosterone, and estradiol concentrations in hen eggs prior to incubation. The embryos from these hormones-treated eggs (HO) as well as sham embryos (O) that had received the vehicle-only were exposed to the odor of fish oil (menhaden) between embryonic Days 11 and 20. An additional group of control embryos (C) was not exposed to the odor. All chicks were tested following hatching for their feeding preferences between foods that were or were not odorized with the menhaden odor. In the 3-min choice tests, the behavior of O chicks differed significantly according to the type of food whereas C and HO chicks showed no preference between odorized and non-odorized food. Our result suggests weaker response in HO chicks. In addition, HO chicks showed impaired growth and reduced intake of an unfamiliar food on the 24-h time scale compared to controls. Our data suggest that embryonic exposure to increased yolk hormone levels can alter growth, chemosensory learning, and the development of feeding behaviors.

Chemoreception.

“…Second, we provide an overview of the anatomical features that are used in chemoreception in a range of invertebrate and vertebrate taxa. Note that many other aspects of biology influence chemosensory behavior, including genetics (Nei, Niimura, & Nozawa, 2008), biochemistry (Wyatt, 2014b), energetics (Palouzier-Paulignan et al, 2012), neurophysiology (Gabor, Phan, Clipperton-Allen, Kavaliers, & Choleris, 2012), and development (Bertin et al, 2016), and we recommend the aforementioned references as introductions to those topics. We chose to focus on anatomy because chemosensory postures are often directly associated with anatomical specializations.…”