Burnout syndrome is a condition in which people work in professions that require face-toface communication and prolonged stress and personal copying methods fail. Healthcare professionals are one of the riskiest occupational groups in terms of burnout syndrome due to their heavy workload. Intense and stressful conditions reduce the joy and productivity of healthcare professionals. In this study, our primary aim is to evaluate the relationship between burnout level and sleep quality in emergency medicine assistants (EMA). This study is a descriptive and cross-sectional study. According to the data obtained from the Higher Education Board, the personal information form and Maslach Burnout Inventory (MBI) and Pittsburgh Sleep Quality Index (PSQI) surveys were sent to all emergency assistants receiving training in Turkey. A total of 94 people working as emergency medical assistants participated in our study. According to the MBI subgroup emotional exhaustion, 84% (n= 79) of the participants had a high level of emotional exhaustion and 16% (15) had moderate emotional exhaustion. When the MBI subgroup desensitization was evaluated, 87.2% (n= 82) was found high and 12.8% (n= 12) was found moderate. When the personal success of the MBI subgroup was evaluated, 97.9% (n= 92) was found low and 2.1% (n= 2) was found moderate. When the correlation between the MBI subgroups and the PSQI scoring was evaluated, a moderate positive correlation was found between emotional exhaustion and PSQI (p≤0.001). When the sleep quality with MBI is evaluated by regression analysis, it is seen that the state of exhaustion affects the quality of sleep by 13.1%. A high level of burnout during the education period of the emergency medicine assistant causes sleep quality deterioration. This situation causes difficulties in business and private life over time. We think that it will be beneficial to regulate the monthly working hours in order to decrease the burnout levels and increase sleep quality for keeping EMAs physically and mentally healthy, to increase the training staff to make them feel safe, and to organize programs to increase non-work social activities.