YODA signalling in the early <i>Arabidopsis</i> embryo

Musielak, Thomas J.; Bayer, Martin

doi:10.1042/bst20130230

Cited by 23 publications

(16 citation statements)

References 40 publications

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“…The yda mutants show a dwarf phenotype because the loss of function of the YDA gene affects zygote elongation and the normal development of the suspensor (the suspensor cells generally have morphology similar to the pro-embryos cells and the large vacuole characteristic of these cells is lacking). As a result, the apical-basal polarity of the future embryo is disrupted (Lukowitz et al 2004;Musielak and Bayer 2014). Conversely, YDA gain-of-function alleles promote exaggerated suspensor growth and suppress embryo development (Lukowitz et al 2004).…”

Section: Molecular View Of Abnormalities In Plant Embryogenesismentioning

confidence: 99%

“…The mpk3 mpk6 double mutants displayed the same phenotype of yda mutants (Musielak and Bayer 2014). It was identified in zygotic embryogenesis that the short suspensor (SSP) and grounded (GRD) genes also act by controlling the YDA-signaling pathway especially the SSP gene that is a member of the interleukin-1 receptor-associated kinase (IRAK)/Pelle-like kinase family of receptors likekinases (Wendrich and Weijers 2013).…”

Section: Molecular View Of Abnormalities In Plant Embryogenesismentioning

confidence: 99%

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Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

105

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Somatic embryogenesis is a morphogenetic event where somatic cells have the ability to produce embryos without gamete fusion. It is used as a technique for plant mass propagation. It is a process that has six well defined steps such as induction, expression, development, maturation, germination and plant conversion. These steps are characterized by distinct physiological, morphological and molecular events. Although somatic embryogenesis has been established in several plant species, there remains many problems to be solved. The main problem in somatic embryogenesis is the large number of abnormal embryos produced which cannot germinate nor convert into normal plants. Abnormalities in somatic embryos (SE) can be generated by genetic or epigenetic changes in the DNA. These changes in the DNA can be influenced by external factors such as the use of plant growth regulators and mutagenic substances or stress factors applied to the plant tissue such as high and low temperatures, drought, salinity, and heavy metals. Abnormalities generated by genetic changes in the DNA are hardly reversible; however, abnormalities generated by epigenetic changes may be reversible and the abnormal embryos are able to produce normal plants in most cases. This review focuses on the identification of the main factors that can cause abnormal SE development in different plant species, suggest how SE abnormalities are related to somaclonal variations and identify which genes may be involved with embryo abnormalities. Zygotic embryo abnormalities in Arabidopsis thaliana mutants are listed with the aim to understand the main genetic mechanisms involved in embryo aberrations. Key messageThe abnormalities in somatic embryos are related to the use of 2,4-D in most of the published protocols, this sintetic auxin disrupts the endogenous auxin balance and the auxin polar transportation interfering with the embryo apical-basal polarity.

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Section: Molecular View Of Abnormalities In Plant Embryogenesismentioning

confidence: 99%

Section: Molecular View Of Abnormalities In Plant Embryogenesismentioning

confidence: 99%

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

105

View full text Add to dashboard Cite

show abstract

“…Over the past 2 decades, studies based primarily on the Arabidopsis model plant, have identified key molecules that include transcription factors, hormones, and micro-RNAs that mediate patterning of the embryo (reviewed in Lau et al, 2012;ten Hove et al, 2015;Jeong et al, 2016;Palovaara et al, 2016). In Arabidopsis, a MAP kinase cascade, mediated by YODA and two MAPKs, acts very early to regulate the first asymmetric cell division of the zygote (Lukowitz et al, 2004;Wang et al, 2007;Musielak and Bayer, 2014). Mutations in YODA and two MAPK genes inhibit elongation of the zygote and cause disruption of the asymmetric first cell division and suspensor cell differentiation.…”

Section: Introductionmentioning

confidence: 99%

Autonomous and non‐autonomous functions of the maize Shohai1 gene, encoding a RWP‐RK putative transcription factor, in regulation of embryo and endosperm development

Mimura

Kudo

et al. 2018

The Plant Journal

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In plants, establishment of the basic body plan during embryogenesis involves complex processes of axis formation, cell fate specification and organ differentiation. While molecular mechanisms of embryogenesis have been well studied in the eudicot Arabidopsis, only a small number of genes regulating embryogenesis has been identified in grass species. Here, we show that a RKD-type RWP-RK transcription factor encoded by Shohai1 (Shai1) is indispensable for embryo and endosperm development in maize. Loss of Shai1 function causes variable morphological defects in the embryo including small scutellum, shoot axis bifurcation and arrest during early organogenesis. Analysis of molecular markers in mutant embryos reveals disturbed patterning of gene expression and altered polar auxin transport. In contrast with typical embryo-defective (emb) mutants that expose a vacant embryo pocket in the endosperm, the endosperm of shai1 kernels conforms to the varied size and shape of the embryo. Furthermore, genetic analysis confirms that Shai1 is required for autonomous formation of the embryo pocket in endosperm of emb mutants. Analyses of genetic mosaic kernels generated by B-A translocation revealed that expression of Shai1 in the endosperm could partially rescue a shai1 mutant embryo and suggested that Shai1 is involved in non-cell autonomous signaling from endosperm that supports normal embryo growth. Taken together, we propose that the Shai1 gene functions in regulating embryonic patterning during grass embryogenesis partly by endosperm-to-embryo interaction.

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“…Previous studies showed that, in the zygote, SSP acts through the YDA MAPKKK and the MAPKs MPK3 and MPK6 (Wang et al 2007;Musielak and Bayer 2014). Therefore, one possible way in which WRKY2 and SSP could interact is that SSP/YDA-MAPK signaling targets the WRKY2 protein.…”

Section: Wrky2 Binds To Mpk3/6 and Is Activated By Phosphorylationmentioning

confidence: 99%

Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote

et al. 2017

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In many plants, the asymmetric division of the zygote sets up the apical-basal axis of the embryo. Unlike animals, plant zygotes are transcriptionally active, implying that plants have evolved specific mechanisms to control transcriptional activation of patterning genes in the zygote. In Arabidopsis, two pathways have been found to regulate zygote asymmetry: YODA (YDA) mitogen-activated protein kinase (MAPK) signaling, which is potentiated by sperm-delivered mRNA of the SHORT SUSPENSOR (SSP) membrane protein, and up-regulation of the patterning gene WOX8 by the WRKY2 transcription factor. How SSP/YDA signaling is transduced into the nucleus and how these pathways are integrated have remained elusive. Here we show that paternal SSP/YDA signaling directly phosphorylates WRKY2, which in turn leads to the up-regulation of WOX8 transcription in the zygote. We further discovered the transcription factors HOMEODOMAIN GLABROUS11/12 (HDG11/12) as maternal regulators of zygote asymmetry that also directly regulate WOX8 transcription. Our results reveal a framework of how maternal and paternal factors are integrated in the zygote to regulate embryo patterning.

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YODA signalling in the early Arabidopsis embryo

Cited by 23 publications

References 40 publications

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Autonomous and non‐autonomous functions of the maize Shohai1 gene, encoding a RWP‐RK putative transcription factor, in regulation of embryo and endosperm development

Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote

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