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Clancy, Michael P.

doi:10.1023/a:1009849401232

Cited by 1 publication

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“…Essentially, it states that biological information contained within either DNA or RNA can be replicated to form exact copies, or can be converted from DNA to RNA or from RNA into proteins (it is possible, although rare, to convert DNA into protein, or RNA into DNA) [2]. Importantly, since DNA contains all of the information a cell needs to grow, survive and reproduce, determining the sequence of a genome will yield information similar to that of looking at RNA or proteins directly.…”

Section: Introductionmentioning

confidence: 99%

Interfacing solid‐state nanopores with gel media to slow DNA translocations

et al. 2015

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We demonstrate the ability to slow DNA translocations through solid‐state nanopores by interfacing the trans side of the membrane with gel media. In this work, we focus on two reptation regimes: when the DNA molecule is flexible on the length scale of a gel pore, and when the DNA behaves as persistent segments in tight gel pores. The first regime is investigated using agarose gels, which produce a very wide distribution of translocation times for 5 kbp dsDNA fragments, spanning over three orders of magnitude. The second regime is attained with polyacrylamide gels, which can maintain a tight spread and produce a shift in the distribution of the translocation times by an order of magnitude for 100 bp dsDNA fragments, if intermolecular crowding on the trans side is avoided. While previous approaches have proven successful at slowing DNA passage, they have generally been detrimental to the S/N, capture rate, or experimental simplicity. These results establish that by controlling the regime of DNA movement exiting a nanopore interfaced with a gel medium, it is possible to address the issue of rapid biomolecule translocations through nanopores—presently one of the largest hurdles facing nanopore‐based analysis—without affecting the signal quality or capture efficiency.

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Section: Introductionmentioning

confidence: 99%