Untitled

Cuadrado, Yolanda; Guerra, Hilario; Martin, Ana Belen; Gallego, Piedad; Hita, Oscar; Dorado, Ana; Villalobos, Nieves

doi:10.1023/a:1011917013886

Cited by 8 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the effect of these sucrose metabolism-related genes on callus formation and shoot regeneration in rice are still unknown. Changes in sucrose- or starch-metabolism–related enzyme activities in callus culture were found in Gossypium hirsutum (Kavi Kishor et al, 1992), Daucus caroata (Tang et al, 1999), Medicago arborea (Cuadrado et al, 2001), and Picea mariana (Iraqi et al, 2005). In our preliminary studies, cellular starch content at callus induction and glucose content at the early regeneration stage were important factors for shoot regeneration in rice (Huang and Liu, 2002; Huang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice (Oryza sativa L.) callus

Lee

Huang

2013

Bot Stud

View full text Add to dashboard Cite

BackgroundShoot regeneration frequency in rice callus is still low and highly diverse among rice cultivars. This study aimed to investigate the association of plant hormone signaling and sucrose uptake and metabolism in rice during callus induction and early shoot organogenesis. The immatured seeds of two rice cultivars, Ai-Nan-Tsao 39 (ANT39) and Tainan 11 (TN11) are used in this study.ResultsCallus formation is earlier, callus fresh weight is higher, but water content is significant lower in ANT39 than in TN11 while their explants are inoculated on callus induction medium (CIM). Besides, the regeneration frequency is prominently higher in ANT39 (~80%) compared to TN11 callus (0%). Levels of glucose, sucrose, and starch are all significant higher in ANT39 than in TN11 either at callus induction or early shoot organogenesis stage. Moreover, high expression levels of Cell wall-bound invertase 1, Sucrose transporter 1 (OsSUT1) and OsSUT2 are detected in ANT39 at the fourth-day in CIM but it cannot be detected in TN11 until the tenth-day. It suggested that ANT39 has higher callus growth rate and shoot regeneration ability may cause from higher activity of sucrose uptake and metabolism. As well, the expression levels of ORYZA SATIVA RESPONSE REGULATOR 1 (ORR1), PIN-formed 1 and Late embryogenesis-abundant 1, representing endogenous cytokinin, auxin and ABA signals, respectively, were also up-regulated in highly regenerable callus, ANT39, but only ORR1 was greatly enhanced in TN11 at the tenth-day in CIM.ConclusionThus, phytohormone signals may affect sucrose metabolism to trigger callus initiation and further de novo shoot regeneration in rice culture.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-54-5) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice (Oryza sativa L.) callus

Lee

Huang

2013

Bot Stud

View full text Add to dashboard Cite

show abstract

Embryo production through somatic embryogenesis can be used to study cell differentiation in plants

Quiroz-Figueroa

Rojas-Herrera

Galáz-Ávalos

et al. 2006

Plant Cell Tiss Organ Cult

221

137

View full text Add to dashboard Cite

Somatic embryogenesis is the process by which somatic cells, under induction conditions, generate embryogenic cells, which go through a series of morphological and biochemical changes that result in the formation of a somatic embryo. Somatic embryogenesis differs from zygotic embryogenesis in that it is observable, its various culture conditions can be controlled, and a lack of material is not a limiting factor for experimentation. These characteristics have converted somatic embryogenesis into a model system for the study of morphological, physiological, molecular and biochemical events occurring during the onset and development of embryogenesis in higher plants; it also has potential biotechnological applications. The focus of this review is on embryo development through somatic embryogenesis and especially the factors affecting cell and embryo differentiation.

show abstract

Developmentally regulated temporal expression and differential acid invertase activity in differentiating cotyledonary explants of mungbean [Vigna radiata (L.) Wilczek]

Maiti

Kundu

Chakraborty

et al. 2011

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Invertases hydrolyze disaccharide-sucrose into glucose and fructose, and are involved in various metabolic processes in plants as key modulators of source/sink activities. Cotyledons of Vigna radiata seeds are photosynthetically inactive sink tissues that store sucrose. However, a sink to source transition occurs when de-embryonated cotyledons are grown under in vitro conditions to undergo differentiation. In this study, two cotyledon types, Cot and Cot E based on a unique differential regeneration response reported earlier, are grown separately in vitro on Gamborg's B5 basal nutrient medium (Gamborg et al. 1968), 2% sucrose, and supplemented with 5, 10, 15, or 20 lM N 6 -benzyladenine (BA). Temporal differences in acid invertase enzyme activity are observed in differentiating explants. In general all BA levels and both in the presence and/or absence of sucrose, acid invertase levels are lower in Cot than Cot E explants. A higher frequency of regeneration of Cot E explants is positively correlated with higher invertase activity.A partial acid invertase (VrAI1) gene has been isolated, cloned, and sequenced (GenBank ID. GQ227550). Using RT-PCR, temporal variations in VrAI1 gene expression are observed in differentiating cotyledons and the transcripts are higher in Cot E than in Cot explants, up to 9 days following culture. Moreover, invertase gene expression is highest in Cot explants at 12 and 15 days following culture. Thus, temporal variations in both amounts of transcripts and in enzyme activity between these two explant types correspond to in vitro responses to shoot differentiation. It is suggested that acid invertase may be involved in temporal differential regeneration patterns observed in Cot and Cot E explants of V. radiata.

show abstract

Untitled

Cited by 8 publications

References 13 publications

Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice (Oryza sativa L.) callus

Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice (Oryza sativa L.) callus

Embryo production through somatic embryogenesis can be used to study cell differentiation in plants

Developmentally regulated temporal expression and differential acid invertase activity in differentiating cotyledonary explants of mungbean [Vigna radiata (L.) Wilczek]

Contact Info

Product

Resources

About