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Abstract

A single-tube, non-interrupted, one-step RT-PCR has been standardized to amplify the hypervariable region of the VP2 gene sequence of infectious bursal disease virus (IBDV). The technique standardized on purified viral RNA was successfully applied to the detection of the virus directly in clinical samples. The amplified products were confirmed to be IBDV specific by their size in ethidium bromide-stained agarose gel, nested PCR and restriction enzyme digestion. Digestion of the amplicons with StyI restriction enzyme also differentiated classical virus from six very virulent field isolates. The sensitivity of the one-step RT-PCR was found to be 0.2 pg of viral RNA.

An exhaustive review on pharmacological potential, bioactive components and formulations of Moringa oleifera

“…The leaf of Moringa oleifera is dorsiventral, meaning that it has two distinct sides: an upper adaxial surface and a lower abaxial surface [38] . The adaxial surface is covered by a waxy cuticle layer and has a layer of epidermal cells with stomata, which are specialized pores that allow for gas exchange [39][40] . The abaxial surface has a layer of loosely packed cells and lacks a cuticle layer.…”