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Assessment of different anther culture approaches to produce doubled haploids in cucumber (Cucumis sativus L.)

“…Our first attempt to promote androgenesis in our cucumber cultivars was to apply some of the few protocols previously published for cucumber anther culture, as well as others published for other cucurbit species (Table 1). Three of the protocols developed for squash anther culture (Shalaby 2006;Metwally et al 1998;Mohamed and Refaei 2004) produced identical results in both Beta Alpha and Esfahani: after two weeks of culture, the 30 cultured anthers swelled and turned yellowish white, but no calli were observed to emerge, becoming necrotic after 6 culture weeks.…”

“…However, it is also true that all these previous studies documented the occurrence of abnormal, but embryo-like structures growing on the callus surface. In our materials and conditions, the protocols published for squash (Kurtar et al 2016;Shalaby 2006;Metwally et al 1998;Mohamed and Refaei 2004) and for different cucurbit interspecific hybrids (Rakha et al 2012) produced very few or no calli at all. The only protocols that consistently promoted callus growth in all anthers of both genotypes were those published for cucumber (Ashok Kumar and Murthy 2004;Song et al 2007;Hamidvand et al 2013;Abdollahi et al 2016;Xie et al 2005).…”

Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)

“…In the case of head cabbage, a significant improvement was obtained in the effectiveness of embryogenesis in anther cultures by increasing the concentration of sucrose to 140 g·L -1 (Górecka, 1998). Metwally et al (1998) were using sucrose at 30 to 150 g·L -1 in a medium for inducing androgenesis in Cucurbita pepo and obtained the best results when the concentration of sucrose was 150 g·L -1…”

Effects of different genotypes and gamma ray doses on haploidization using irradiated pollen technique in squash

“…There are 3 haploidy techniques: androgenesis (anther culture, microspore culture), gynogenesis (ovule and ovarium culture), and parthenogenesis (irradiated pollination technique). Anther culture, ovule culture, and ovarium culture studies in different species of the family Cucurbitaceae have been performed (Gémesné and Venczel, 1996;Metwally et al, 1998aMetwally et al, , 1998bMohamed and Refaei, 2004). However, haploid embryos could not be obtained via anther culture, and only a few haploid embryos were observed in ovule and ovarium culture.…”