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Viktorov, I. V.; Proshin, S. S.

doi:10.1023/a:1026017719668

Cited by 17 publications

(12 citation statements)

References 2 publications

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“…Regarding the tissue quality, we assessed the neuropil and cellular shape since these characteristics reflect the changes in cell morphology that may be impacted by the different chemicals used for fixation (Troiano et al, 2009;Spencer and Bancroft, 2017). We speculate that the shrunken aspect of the neuronal cell bodies observed in SSS brains, but not in the FAS nor AS-fixed specimens, is related to the high concentration of isopropyl alcohol, which is a potent dehydrating fluid, only present in the SSS (Viktorov and Proshin, 2003). In addition, isopropyl alcohol could have also affected the higher occurrence of fissured neuropil in these brains.…”

Section: Tissue Qualitymentioning

confidence: 99%

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Frigon

Dadar²,

Boire³

et al. 2022

Front. Neuroanat.

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BackgroundHistology remains the gold-standard to assess human brain biology, so ex vivo studies using tissue from brain banks are standard practice in neuroscientific research. However, a larger number of specimens could be obtained from gross anatomy laboratories. These specimens are fixed with solutions appropriate for dissections, but whether they also preserve brain tissue antigenicity is unclear. Therefore, we perfused mice brains with solutions used for human body preservation to assess and compare the tissue quality and antigenicity of the main cell populations.Materials and methodsTwenty-eight C57BL/6J mice were perfused with 4% formaldehyde (FAS, N = 9), salt-saturated solution (SSS, N = 9), and alcohol solution (AS, N = 10). The brains were cut into 40 μm sections for antigenicity analysis and were assessed by immunohistochemistry of four antigens: neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP astrocytes), ionized calcium-binding adaptor molecule 1 (Iba1-microglia), and myelin proteolipid protein (PLP). We compared the fixatives according to multiple variables: perfusion quality, ease of manipulation, tissue quality, immunohistochemistry quality, and antigenicity preservation.ResultsThe perfusion quality was better using FAS and worse using AS. The manipulation was very poor in SSS brains. FAS- and AS-fixed brains showed higher tissue and immunohistochemistry quality than the SSS brains. All antigens were readily observed in every specimen, regardless of the fixative solution.ConclusionSolutions designed to preserve specimens for human gross anatomy dissections also preserve tissue antigenicity in different brain cells. This offers opportunities for the use of human brains fixed in gross anatomy laboratories to assess normal or pathological conditions.

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Section: Tissue Qualitymentioning

confidence: 99%

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Frigon

Dadar²,

Boire³

et al. 2022

Front. Neuroanat.

View full text Add to dashboard Cite

show abstract

“…Regarding the tissue quality, we assessed the neuropil and cellular shape since these characteristics reflect the changes of cell morphology that may be impacted by the different chemicals used for fixation (Spencer, 2017; Troiano, Ciovacco, & Kacena, 2009). We speculate that the shrunken aspect of the neuronal cells bodies observed in SSS brains, but not in the FAS nor AS-fixed specimens, is related to the high concentration of isopropylic alcohol, which is a potent dehydrating fluid, only present in the SSS (Viktorov & Proshin, 2003). Additionally, isopropylic alcohol could have also affected the higher occurrence of fissured neuropil in these brains.…”

Section: Discussionmentioning

confidence: 84%

“…Additionally, isopropylic alcohol could have also affected the higher occurrence of fissured neuropil in these brains. In the AS-fixed brains, the higher frequency of fissured neuropil may be due to the high concentration of ethanol, also used as a dehydrating agent (Viktorov & Proshin, 2003). However, despite the poorer tissue quality of AS and SSS-fixed brains, all specimens clearly showed the targeted cells (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Frigon

Dadar

Boire

et al. 2022

Preprint

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Background: Histology remains the gold standard to assess human brain biology, so ex vivo studies using tissue from brain banks are standard practice in neuroscientific research. However, a larger number of specimens could be obtained from gross anatomy laboratories. These specimens are fixed with solutions appropriate for dissections, but whether they also preserve brain tissue antigenicity is unclear. Therefore, we perfused mice brains with solutions used for human body preservation to assess and compare the tissue quality and antigenicity of the main cell populations. Methods: 28 C57BL/6J mice were perfused with: 4% formaldehyde (FAS, N=9), salt-saturated solution (SSS, N=9), and alcohol-solution (AS, N=10). The brains were cut into 40μm sections for antigenicity analysis and were assessed by immunohistochemistry of four antigens: neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP-astrocytes), ionized calcium binding adaptor molecule1 (Iba1-microglia), and myelin proteolipid protein (PLP). We compared the fixatives according to multiple variables: perfusion quality, ease of manipulation, tissue quality, immunohistochemistry quality, and antigenicity preservation. Results: The perfusion quality was better using FAS and worse using AS. The manipulation was very poor in SSS brains. FAS and AS fixed brains showed higher tissue and immunohistochemistry quality than the SSS brains. All antigens were readily observed in every specimen, regardless of the fixative solution. Conclusion: Solutions designed to preserve specimens for human gross anatomy dissections also preserve tissue antigenicity in different brain cells. This offers opportunities for the use of human brains fixed in gross anatomy laboratories to assess normal or pathological conditions.

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“…Isopropyl alcohol used in different grades (50%, 70%, 90%, 100%) is a good and cost-effective alternative to ethyl alcohol in tissue processing. In our experience, tissue floating was observed using isopropyl alcohol; therefore, its use should be discouraged in manual IHC staining [ 9 , 10 ]. During the process, the tissue undergoes fixation and dehydration, and ethyl alcohol used in different grades (50%, 70%, 90%, 100%) is found to accurate fixative as well as dehydration solution, so no tissue floatation was seen [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Standardization of Manual Method of Immunohistochemical Staining for Breast Cancer Biomarkers at Tertiary Cancer Care Center: An Audit

Rana

Jain

et al. 2022

Cureus

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Untitled

Cited by 17 publications

References 2 publications

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Standardization of Manual Method of Immunohistochemical Staining for Breast Cancer Biomarkers at Tertiary Cancer Care Center: An Audit

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