Yifei Sanjie Formula Treats Chronic Obstructive Pulmonary Disease by Remodeling Pulmonary Microbiota

Abstract

Chronic obstructive pulmonary disease (COPD) is one of the most common pulmonary diseases. Evidence suggests that dysbiosis of pulmonary microbiota leads to the COPD pathological process. Yifei Sanjie Formula (YS) is widely used to treat diseases in respiratory systems, yet little is known about its mechanisms. In the present study, we first established the fingerprint of YS as the background for UHPLC-QTOF-MS. Components were detected, including alkaloids, amino acid derivatives, phenylpropanoids, flavonoids, terpenoids, organic acids, phenols, and the like. The therapeutic effect of YS on COPD was evaluated, and the pulmonary function and ventilatory dysfunction (EF50, TV, and MV) were improved after the administration of YS. Further, the influx of lymphocytes was inhibited in pulmonary parenchyma, accompanied by down-regulation of inflammation cytokines via the NLRP3/caspase-1/IL-1β signaling pathway. The severity of pulmonary pathological damage was reversed. Disturbed pulmonary microbiota was discovered to involve an increased relative abundance of Ralstonia and Mycoplasma and a decreased relative abundance of Lactobacillus and Bacteroides in COPD animals. However, the subversive effect was shown. The abundance and diversity of pulmonary microflora were remodeled, especially increasing beneficial genua Lactobacillus and Bacteroides, as well as downregulating pathogenic genua Ralstonia and Mycoplasma in the YS group. Environmental factor correlation analysis showed that growing pulmonary microbiota was positively correlated with the inflammatory factor, referring to Ralstonia and Mycoplasma, as well as negatively correlated with the inflammatory factor, referring to Lactobacillus and Bacteroides. These results suggest that the effects of YS involved remodeling lung microbes and anti-inflammatory signal pathways, revealing that intervention microbiota and an anti-inflammatory may be a potential therapeutic strategy for COPD.

Yifei Sanjie formula alleviates lung cancer progression via regulating PRMT6‐YBX1‐CDC25A axis

“…Numerous studies have shown that YFSJ is a widely used Chinese herbal medicine for treating lung related diseases. 7,8,12,13 The YFSJ formula, a TCM, has been shown to have a broad pharmacological action against lung diseases. It has been found to lower inflammatory responses and reduce lung damage.…”

“…TCM has an extensive track record of effectively treating lung cancer with minimal adverse effects and increased patient survival, 43 which justifies further comprehensive investigation. Numerous studies have shown that YFSJ is a widely used Chinese herbal medicine for treating lung related diseases 7,8,12,13 …”

“…Yifei Sanjie (YFSJ), also known as “Yiqi Chutan Tang,” is a TCM formula that comprises eight medicinal herbs (Hedysarum Multijugum Maxim, Atractylodes Macrocephala Koidz, Saposhnikoviae Radix, Sinapis Semen, Fritillariae Thunbrgii Bulbus, Mori Cortex, Curcumae Rhizoma, and Panax Notoginseng [Burk.] F. H. Chen Ex C. Chow) 7,8 . Numerous pharmacological effects of YFSJ against pulmonary disease have been demonstrated, including a decrease in inflammatory responses and lung injury 9–11 .…”

“…F. H. Chen Ex C. Chow). 7,8 Numerous pharmacological effects of YFSJ against pulmonary disease have been demonstrated, including a decrease in inflammatory responses and lung injury. [9][10][11] In a case control study of 376 NSCLC patients, it was found that YFSJF plus chemotherapy improves the median overall survival compared with chemotherapy alone.…”

Rice-fish symbiosis improves the muscle nutrition and intestinal flora diversity of tilapia

“…At the same time, Ralstonia is an important inflammatory factor in the intestine. [27][28][29][30][31][32] The study further showed that the abundance of Pseudomonas and Ralstonia in the R group was significantly higher than in the T group (p<0.05). We speculate that the composition of tilapia intestinal flora in the rice-fish symbiosis mode is more conducive to fish health.…”