Yield optimization and rational function modelling of enzymatic hydrolysis of wheat straw pretreated by NaOH-delignification, autohydrolysis and their combination

Pihlajaniemi, Ville; Sipponen, Mika H.; Pastinen, Ossi; Lehtomäki, Ilkka; Laakso, Simo

doi:10.1039/c4gc02218a

Cited by 23 publications

(27 citation statements)

References 42 publications

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“…In accordance with our previous results (Pihlajaniemi et al, 2015), the proportional neutralization of the NaOH-feed was decreased as the NaOH-loading was increased (Fig. 4A).…”

Section: Naoh-consumptionsupporting

confidence: 92%

“…Relatively low hemicellulose dissolution was observed, with up to 12% dissolution in percolation (Fig. 5A), which is at a similar level with previous reports of batch reactions at a higher thermal severity (Chen et al, 2013;Pihlajaniemi et al, 2015). In the batch reactions, only up to 5% of hemicellulose was dissolved.…”

Section: Dissolution Of Hemicellulosesupporting

confidence: 89%

“…For further perspective of the benefit of the flow-through processes, the yields were compared to our previous results from batch delignification of the same straw at higher thermal severities in a pressurized reaction at 140 C for 1 h (Pihlajaniemi et al, 2015). Figure 3B presents the current sugar yields with 8 FPU g À1 DM and the corresponding enzyme productivities (mg sugar per FPU) compared to previous yield-optimization curves for enzyme consumption, at enzyme dosages of 4, 8, and 12 FPU g À1 DM.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

“…Wheat straw from Finland with the composition of 39.0% cellulose, 23.7% xylan, and 23.7% lignin (Pihlajaniemi et al, 2015) was chopped with a hammer mill to pass a 10 mm screen and delignified by a batch reaction or by a flow-through process, either by regular or counter-current percolation. Each counter-current process was performed as a progressing sequence of 8-10 reactions, where a single NaOH-loading passed through a cascade of three columns, and accordingly, three NaOH-loadings passed through each column at a triple flow rate compared to regular percolation, as illustrated in Figure 1 (Lee et al, 1999).…”

Section: Delignification Reactionsmentioning

confidence: 99%

“…Different pretreatments have been developed to overcome the recalcitrance of the materials, ranging from hydrothermal or acidic hydrolysis and steam explosion to delignification by alkaline chemicals, oxidative reagents, and organic solvents. Delignification methods have shown a higher sugar yield potential compared to hydrothermal methods (Chen et al, 2013;Nguyen et al, 2015;Pihlajaniemi et al, 2015), but they suffer from the costs of the addition and recycling of chemicals. Alkaline delignification with NaOH is analogous to soda pulping (Lora, 2008) and typically NaOH-loadings above 8% per dry matter are preferred for agricultural residues (Chen et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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The effect of direct and counter‐current flow‐through delignification on enzymatic hydrolysis of wheat straw, and flow limits due to compressibility

Pihlajaniemi

Sipponen

Pastinen

et al. 2016

Biotech & Bioengineering

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This article compares the processes for wheat straw lignocellulose fractionation by percolation, counter-current progressing batch percolation and batch reaction at low NaOH-loadings (3-6% of DM). The flow-through processes were found to improve delignification and subsequent enzymatic saccharification, reduce NaOH-consumption and allow reduction of thermal severity, whereas hemicellulose dissolution was unaffected. However, contrary to previous expectations, a counter-current process did not provide additional benefits to regular percolation. The compressibility and flow properties of a straw bed were determined and used for simulation of the packing density profile and dynamic pressure in an industrial scale column. After dissolution of 30% of the straw DM by delignification, a pressure drop above 100 kPa m led to clogging of the flow due to compaction of straw. Accordingly, the maximum applicable feed pressure and volumetric straw throughput was determined as a function of column height, indicating that a 10 m column can be operated at a maximum feed pressure of 530 kPa, corresponding to an operation time of 50 min and a throughput of 163 kg m h . Biotechnol. Bioeng. 2016;113: 2605-2613. © 2016 Wiley Periodicals, Inc.

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“…In accordance with our previous results (Pihlajaniemi et al, 2015), the proportional neutralization of the NaOH-feed was decreased as the NaOH-loading was increased (Fig. 4A).…”

Section: Naoh-consumptionsupporting

confidence: 92%

Section: Dissolution Of Hemicellulosesupporting

confidence: 89%

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Section: Delignification Reactionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The effect of direct and counter‐current flow‐through delignification on enzymatic hydrolysis of wheat straw, and flow limits due to compressibility

Pihlajaniemi

Sipponen

Pastinen

et al. 2016

Biotech & Bioengineering

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Performances of Several Solvents on the Cleavage of Inter‐ and Intramolecular Linkages of Lignin in Corncob Residue

Zhang

et al. 2018

ChemSusChem

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The performances of solvents, including γ-butyrolactone (GBL), γ-valerolactone (GVL), tetrahydrofuran (THF), ethyl acetate (EAC), 2-methyltetrahydrofuran (2-MeTHF), and the corresponding mixtures with H O, on the cleavage of inter- and intramolecular linkages of lignin in corncob residue were investigated. At 200 °C, miscible cosolvents (H O-GBL, H O-GVL, and H O-THF) exhibited much better efficiency for lignin dissolution than that of both immiscible cosolvents (H O-EAC and H O-2-MeTHF) and pure solvents. The synergetic effect between H O and organic solvent significantly promoted the breakage of intermolecular linkages between C6-O-H of amorphous cellulose and lignin. GBL and THF solvents preferentially dissolved lignin with H and G units, whereas GVL, EAC, and 2-MeTHF solvents exhibited high selectivity for the dissolution of lignin with S and G units. In addition to dissolution, the intramolecular β-O-4 linkage in lignin could be selectively cleaved in H O-GBL cosolvent, whereas the β-O-4, α-O-4, and β-5 linkages were cleaved in H O-EAC, H O-THF, and H O-2-MeTHF cosolvents. At 300 °C, the breakage of the β-γ bond prior to β-O-4 in H O-GBL, H O-THF, H O-EAC, and H O-2-MeTHF produced 4-ethylphenol and 4-ethylguaiacol selectively (accounting for ≈70 % of the total identified monophenols), whereas the α-1 bond was preferably broken in H O-GVL to form guaiacol (accounting for ≈75 % of the total identified monophenols).

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In operando monitoring of wood transformation during pretreatment with ionic liquids

et al. 2020

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The conversion of lignocellulosic biomass or wood into chemicals still poses a challenge due to the recalcitrance of this composite-like material consisting of lignin, hemicellulose and cellulose. A very high accessibility of cellulose is reported by a pretreatment with ionic liquids that enables high conversion rates by enzymatic hydrolysis. However, the underlying mechanisms have not yet been monitored in operando nor are they fully understood. We monitored the transformation of wood in ionic liquids using small-angle neutron scattering to observe changes in the material in operando and to elucidate the intrinsic effects. The data analysis shows three different stages that is (1) impregnation, (2) the formation of voids and (3) increasing structure size within cellulose fibrils. This consecutive mechanism coincides with macroscopic disintegration of the tissue. The analysis further reveals that the reduction of order in longitudinal direction along the fiber axis is a prerequisite for disintegration of cells along the radial direction. This understanding supports further research and development of pretreatment processes starting from lignocellulosic raw material.

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Yield optimization and rational function modelling of enzymatic hydrolysis of wheat straw pretreated by NaOH-delignification, autohydrolysis and their combination

Abstract: The sugar yield of saccharification processes must be optimized against productivity, for which rational function modelling provides a smooth solution.

Cited by 23 publications

References 42 publications

The effect of direct and counter‐current flow‐through delignification on enzymatic hydrolysis of wheat straw, and flow limits due to compressibility

The effect of direct and counter‐current flow‐through delignification on enzymatic hydrolysis of wheat straw, and flow limits due to compressibility

Performances of Several Solvents on the Cleavage of Inter‐ and Intramolecular Linkages of Lignin in Corncob Residue

In operando monitoring of wood transformation during pretreatment with ionic liquids

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