Yidiyin, a Chinese Herbal Decoction, Improves Erectile Dysfunction in Diabetic Patients and Rats through the NO-cGMP Pathway

Feng, Xiaobing; Can-bin, Qin; Leng, Jing; Tang, Qianli; Shi, Hong; Zhai, Lin-Na; Li, Shuanglei

doi:10.1271/bbb.110568

Cited by 16 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The protein content per mg tissue was determined with a coomassie brilliant blue protein assay kit, according to the manufacturer's instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). 20,21 Absorbance was measured at 550 nm in a microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) and the NO level was detected according to the standard graph of sodium nitrite (in the range 1-10 mmole ml À 1 ).…”

Section: No Production Assaymentioning

confidence: 99%

Panax notoginseng saponins improve the erectile dysfunction in diabetic rats by protecting the endothelial function of the penile corpus cavernosum

Lin

Gou

2013

Int J Impot Res

View full text Add to dashboard Cite

Diabetes mellitus (DM)-associated ED is predominantly due to neurovascular dysfunction mediated by nitric oxide (NO) suppression. Panax notoginseng saponins (PNS) are widely used for treating cardiovascular disease in China. The aim of this study was to evaluate the effects of PNS on penile erection and corpus cavernosum tissues in rats with diabetes-associated ED. Four weeks after PNS treatment, erectile function was assessed by intracavernous pressure (ICP) and mean arterial pressure (MAP) measurements. The level of NO, cyclic guanosine monophosphate (cGMP) and advanced glycation end products (AGEs) in cavernous tissue were assessed. Immunohistochemical staining and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) were performed for detecting endothelial NO synthase (eNOS) and apoptosis, respectively. The results show that ICP/MAP ratio was significantly increased in high-dose (150 mg kg(-1) per day) PNS-treated group compared with the diabetic ED untreated group (DM group). Compared with the untreated group, the expression of eNOS and the levels of NO and cGMP were increased in the PNS-treated groups. Moreover, apoptosis was markedly decreased in the group that received 150 mg kg(-1) per day of PNS. These results suggest that PNS may be used for improving the ED in diabetic rats via the NO/cGMP pathway and restores the function of endothelium in corpus cavernosum.

show abstract

Section: No Production Assaymentioning

confidence: 99%

Panax notoginseng saponins improve the erectile dysfunction in diabetic rats by protecting the endothelial function of the penile corpus cavernosum

Lin

Gou

2013

Int J Impot Res

View full text Add to dashboard Cite

show abstract

“…Recently, many studies have associated eNOS and nNOS with the level of erectile function [4], [30]. Accordingly, BCH-induced ED showed lower ICP without recovery, and the NOS levels were consistently lower than normal.…”

Section: Discussionmentioning

confidence: 99%

A Novel Method to Establish a Rat ED Model Using Internal Iliac Artery Ligation Combined with Hyperlipidemia

Wang

Dong

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

ObjectiveTo investigate a novel method, namely using bilateral internal iliac artery ligation combined with a high-fat diet (BCH), for establishing a rat model of erectile dysfunction (ED) that, compared to classical approaches, more closely mimics the chronic pathophysiology of human ED after acute ischemic insult.Materials and MethodsForty 4-month-old male Sprague Dawley rats were randomly placed into five groups (n = 8 per group): normal control (NC), bilateral internal iliac artery ligation (BIIAL), high-fat diet (HFD), BCH, and mock surgery (MS). All rats were induced for 12 weeks. Copulatory behavior, intracavernosal pressure (ICP), ICP/mean arterial pressure, hematoxylin-eosin staining, Masson's trichrome staining, serum lipid levels, and endothelial and neuronal nitric oxide synthase immunohistochemical staining of the cavernous smooth muscle and endothelium were assessed. Data were analyzed by SAS 8.0 for Windows.ResultsSerum total cholesterol and triglyceride levels were significantly higher in the HFD and BCH groups than the NC and MS groups. High density lipoprotein levels were significantly lower in the HFD and BCH groups than the NC and MS groups. The ICP values and mount and intromission numbers were significantly lower in the BIIAL, HFD, and BCH groups than in the NC and MS groups. ICP was significantly lower in the BCH group than in the BIIAL and HFD groups. Cavernous smooth muscle and endothelial damage increased in the HFD and BCH groups. Cavernous smooth muscle to collagen ratio, nNOS and eNOS staining decreased significantly in the BIIAL, HFD, and BCH groups compared to the NC and MS groups.ConclusionsThe novel BCH model mimics the chronic pathophysiology of ED in humans and avoids the drawbacks of traditional ED models.

show abstract

“…Combined use of Yidiyin and hypoglycemic drugs could increase patients’ scores on international index of erectile function-5 (IIEF-5) more than the hypoglycemic drug alone. Animal experiments indicated that administration of Yidiyin increased rats’ erectile function through activating NOS-cGMP pathway (45). …”

Section: Chinese Herbal Treatment For Edmentioning

confidence: 99%

Traditional Chinese medical therapy for erectile dysfunction

Li¹,

Jiang²,

Liu³

2017

Transl. Androl. Urol.

View full text Add to dashboard Cite

Traditional Chinese medicine (TCM), including acupuncture and Chinese herbs, is used as an alternative therapy to increase the curative effect for erectile dysfunction (ED). A large number of studies have been conducted to investigate the effect and mechanism of TCM for treating ED. The therapeutic effect of acupuncture on ED is still controversial at present. However, some Chinese herbs exhibited satisfying outcomes and they might improve erectile function by activating nitric oxide synthase (NOS)-cyclic guanosine monophosphate (cGMP) pathway, increasing cyclic adenosine monophosphate (cAMP) expression, elevating testosterone level, reducing intracellular Ca2+ concentration, down-regulating transforming growth factor β1 (TGFβ1)/Smad2 signaling pathway, or ameliorating the oxidative stress.

show abstract

Yidiyin, a Chinese Herbal Decoction, Improves Erectile Dysfunction in Diabetic Patients and Rats through the NO-cGMP Pathway

Cited by 16 publications

References 35 publications

Panax notoginseng saponins improve the erectile dysfunction in diabetic rats by protecting the endothelial function of the penile corpus cavernosum

Panax notoginseng saponins improve the erectile dysfunction in diabetic rats by protecting the endothelial function of the penile corpus cavernosum

A Novel Method to Establish a Rat ED Model Using Internal Iliac Artery Ligation Combined with Hyperlipidemia

Traditional Chinese medical therapy for erectile dysfunction

Contact Info

Product

Resources

About