Yi Guan Jian, a Traditional Chinese Herbal Medicine, Alleviates Carbon Tetrachloride-Induced Liver Injury

Abstract

Objective. To study the protective effect of Yi Guan Jian (YGJ) on liver injury induced by carbon tetrachloride (CCl4) in mice. Methods. 50 ICR mice were randomly divided into 5 groups: control group, carbon tetrachloride (CCl4) group, CCl4 + silymarin Group (200 mg/kg), carbon CCl4 + YGJ Group (11.5, 23 g/kg). Except the mice in the control group and the CCl4 group that were given the same volume of distilled water, the mice in other groups were given the drugs for seven days. 2 hours after the last administration, except the mice in the control group, the mice in other groups had intraperitoneal injection of 0.1% CCl4 vegetable oil solution (10 ml/kg). Mice in control group had intraperitoneal injection of the same volume of vegetable oil. After 18h, the blood and liver were collected. The liver of mice was stained with HE staining, the levels of alanine transaminase (ALT) and glutamic pyruvic transaminas (AST) in serum were detected, malondialdehyde (MDA), superoxide dismutase (SOD), interleukin (IL-6, Il-1β), and tumor necrosis factor (TNF-α) in serum and liver were detected, and the Western blot was used to detect the levels of MAPK/NF-κB pathway. Results. YGJ significantly decreased the levels of ALT and AST. The contents of MDA, IL-6, Il-1β, and TNF-α in serum and liver were significantly decreased and the contents of SOD in serum and liver were significantly decreased by YGJ, which significantly improved the pathological changes of liver tissue in mice. The levels of MAPK/NF-κB pathway were significantly decreased by YGJ. Conclusion. YGJ has protective effect on CCl4 induced liver injury in mice, which may be related to the MAPK/NF-κB signaling pathway.

Ethanol Leaf Extract of Acrostichum aureum Modifies Carbon Tetrachloride-induced Oxidative Stress and Hepato-renal Damage in Rats

“…chloromethane, dichlorocarbene, and trichloromethyl peroxyl radicals. [3][4][5] These radicals can either bind directly to tissues or initiate a cascade of free radical-mediated reactions leading to lipid peroxidation. Therefore, the elevation MDA observed in the CCl4-treated group is a manifestation of CCl4-induced lipid peroxidation.…”

Network pharmacology-based analysis on the key mechanisms of Yiguanjian acting on chronic hepatitis

“…YGJ has been widely used in the treatment of chronic liver diseases, including CH, liver fibrosis, and cirrhosis. YGJ has been shown to effectively address chronic liver injury by targeting the MAPK/NF-κB pathway [ 9 ]. It regulates the Wnt signaling pathway to suppress astrocyte activation, thereby mitigating liver fibrosis and impeding cirrhosis progression [ 10 ].…”

“…Clinical studies revealed that YGJ not only alleviated clinical symptoms in patients with liver disease but also reduced serum alanine transaminase (ALT) and aspartate transaminase (AST) levels. Although numerous studies have demonstrated the significant role of YGJ in reducing liver inflammation, restoring liver function, and treating CH, the precise mechanism of its action remains unclear [ 9 , 10 ].…”

Mechanism of hydroxysafflor yellow A on acute liver injury based on transcriptomics

“…Moreover, these ROS are capable of attacking fatty acids and causing peroxidation (Li et al, 2019). As the metabolic endproduct of lipid peroxides, MDA can aggravate cell membrane damage by destroying the function, structure, and metabolism of biofilms (Lixin et al, 2019). SOD is the principal antioxidative enzyme, which can transform free radicals into water and molecular oxygen, thus reducing cell damage (Wu et al, 2017).…”