YFR016c/Aip5 is part of an actin nucleation complex in budding yeast cells

Abstract

statementYFR016c/Aip5 binds to the polarisome components Bud6 and Spa2 and supports the polarisome in the formation of actin filaments in yeast cells. AbstractThe polarisome comprises a network of proteins that organizes polar growth in yeast and filamentous fungi. The yeast Saccharomyces cerevisiae formin Bni1 and the actin-nucleation-promoting factor Bud6 are subunits of the polarisome that together catalyse the formation of actin filaments below the tip of budding yeast cells. We identified YFR016c (Aip5) as interaction partner of Bud6 and the polarisome scaffold Spa2. Yeast cells lacking Aip5 display a reduced number of actin cables. Aip5 binds with its N-terminal region to Spa2 and with its C-terminal region to Bud6. Both interactions collaborate to localize Aip5 at bud tip and neck, and are required to stimulate the formation of actin cables. Our experiments characterize Aip5 as a novel subunit of a complex that regulates the number of actin filaments at sites of polar growth.