Yessotoxin induces apoptosis in HL7702 human liver cells

Pang, Min; Qü, Ping; Gao, Chunlei; Wang, Zongling

doi:10.3892/mmr.2011.606

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…However, in the tumor model erythroleukemia K-562 cell line, YTX did not directly modify cytosolic calcium levels, but it increased thapsigargin-independent calcium pools depletion, as well as the influx through store operated calcium channels [ 35 ]. A cytosolic calcium increase in the presence of YTX was also observed in HL7702 human liver cells, Bel7402 human hepatoma cell line and in primary cultures of rat cerebellar neurons [ 36 , 37 , 38 , 39 ]. However, no direct modification in calcium basal levels was observed in rat primary cardiomyocytes after YTX incubation [ 40 ].…”

Section: Mechanism Of Action Of Ytxmentioning

confidence: 81%

“…The apoptotic effect of YTX had been reported for the first time in 2002 in BE(2)-M17 neuroblastoma cell line [ 42 ]. Later, several apoptosis hallmarks in many different cell types, such as HeLa S 3 cells, cerebellar neurons, L6 and BC3H1 myoblasts, mouse fibroblasts NIH3T3, MDCK kidney cells and MCF-7 breast cells, HepG2, Bel7402 and HL7702 human hepatoma cell lines, and liver cells have been published [ 37 , 38 , 39 , 43 , 59 , 60 , 61 , 62 , 63 , 64 ]. In the erythroleukemia K-562 cell line the apoptotic cell death was activated after 24 h YTX incubation when the AKAP 149-PKA-PDE4A complex was located in the plasma membrane [ 55 ].…”

Section: Cellular Death and Ytxmentioning

confidence: 99%

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Yessotoxin, a Promising Therapeutic Tool

2016

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Yessotoxin (YTX) is a polyether compound produced by dinoflagellates and accumulated in filter feeding shellfish. No records about human intoxications induced by this compound have been published, however it is considered a toxin. Modifications in second messenger levels, protein levels, immune cells, cytoskeleton or activation of different cellular death types have been published as consequence of YTX exposure. This review summarizes the main intracellular pathways modulated by YTX and their pharmacological and therapeutic implications.

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Section: Mechanism Of Action Of Ytxmentioning

confidence: 81%

Section: Cellular Death and Ytxmentioning

confidence: 99%

Yessotoxin, a Promising Therapeutic Tool

2016

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“…Hoechst 33824 staining was performed to investigate the effect of baicalein on the apoptosis of HL-7702 cells induced by 60.5 mM of glucose [24]. In apoptotic cells, the condensed chromatin was stained with Hoechst 33824 and elicited strong blue fluorescence upon excitation.…”

Section: The Effect Of Baicalein On the Apoptosis Of High Glucose-indmentioning

confidence: 99%

Baicalein Alleviates Liver Oxidative Stress and Apoptosis Induced by High-Level Glucose through the Activation of the PERK/Nrf2 Signaling Pathway

et al. 2020

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Baicalein, a widely-distributed natural flavonoid, exhibits antioxidative activity in mice with type-2 diabetes. However, the underlying mechanisms remain partially elucidated. In this study, we investigated the effect of baicalein on protein kinase R-like ER kinase (PERK)/nuclear factor erythroid-2-related factor 2 (Nrf2) pathway for the alleviation of oxidative stress and apoptosis. Human liver HL-7702 cells were stimulated with 60.5 mM of glucose to induce oxidative stress and treated with baicalein. The apoptosis was determined by fluorescence microscopy and flow cytometry. The regulation of the PERK/Nrf2 pathway by baicalein was determined by immunoblotting in both HL-7702 cells and liver tissues from diabetic mice. We found that baicalein significantly alleviated the oxidative stress and apoptosis in HL-7702 cells stimulated with glucose. Mechanistic studies showed that baicalein downregulated PERK and upregulated Nrf2, two key proteins involved in endoplasmic reticulum stress, in both HL-7702 cells and liver tissues from diabetic mice receiving baicalein treatment. Furthermore, the subcellular localization of Nrf2 and the regulation of downstream proteins including heme oxygenase-1 and CCAAT-enhancer-binding protein homologous protein (CHOP) by baicalein were also investigated. Our results suggest that the regulation of the PERK/Nrf2 pathway is one of the mechanisms contributing to the bioactivities of baicalein to improve diabetes-associated complications.

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“…Another test aims at dosedependent accumulation of e-cadherin fragments in MCF-7 cells upon Ytx treatment giving a detection range of 0.2 to 1.8 mg Ytxequiv./kg (Schirone et al, 2013). Furthermore, the apoptotic activity of Ytx with regard to chromatin condensation, DNA laddering, caspase-3 activation, disruption of mitochondrial membrane potential and increase of cytosolic calcium levels could be revealed providing a potential for development of functional assays (Pang et al, 2012). Further findings pointing to inhibitory potency of Ytx with regard to phagocytosis of macrophages (Orsi et al, 2010) or new binding proteins of Ytx (Ujihara et al, 2010) may open the door for further research in the field of YSP.…”

Section: Human-relevant Functional Reference Systemsmentioning

confidence: 99%

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Daneshian

2013

ALTEX

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Summary Aquatic food accounts for over 40% of global animal food products, and the potential contamination with toxins of algal origin -marine biotoxins -poses a health threat for consumers. The gold standards to assess toxins in aquatic food have traditionally been in vivo methodssingle marine biotoxins or combinations of marine biotoxins and intoxication symptomatology as well as monitoring the "success" of controls implemented during fishery and aquaculture production, processing, and retailing. For this reason, a workshop was organized in ermatingen, Switzerland by the Center for Alternatives to Animal testing -europe (CAAteurope) within the framework of the transatlantic think tank for toxicology (t 4 ).In contrast to other food products where hygiene and potential contamination with microbes or mold toxins is the prime issue, the emphasis in aquatic products (shellfish and finfish) is, beyond viral and bacterial contaminations, primarily placed on potential contamination with marine biotoxins owing to the numerous and recurring human intoxications.The gold standards to assess toxins in aquatic food have traditionally been in vivo methods, i.e., the mouse and the rat bioassays. Besides the ethical issues of in vivo bioassays there are specific difficulties, e.g., different exposure route (i.p. versus oral), low inter-species comparability, high intra-species variability, and questionable extrapolation of quantitative risk to humans, thus highlighting the need for the development of more reliable detection and quantification methods. Based on this reasoning, the european Food Safety Authority (eFSA) advocates the use of an analytical method, i.e., LC-MS (Liquid Chromatography coupled Mass Spectrometry), as a substitute for in vivo bioassays for almost all classes of marine toxins. However, although lC-MS is a very sensitive method that has the advantages of being able to detect multiple toxins in a single analysis and being good for confirming the identity of toxins, the quality of quantitative analysis is dependent on the availability of calibration standards. While many new toxin structural analogues have been detected and identified using LC-MS, this technique -as with most other methods -is not good at detecting new toxin types. When new toxin analogues are detected but accurate standards are not yet available, only an approximate quantitation is possible using estimated response factors.For the purpose of risk assessment of food, however, it is imperative to focus on the possible adverse effects, independent of whether they stem from one toxin or a combination of toxins. As toxicity is defined by functional and structural changes of biological systems, the development of a human-relevant in vitro system for appropriate risk assessment of marine biotoxins in seafood stands to reason. Moreover, poor correlation of human intoxications, of acute symptomatology and long-term adverse effects, and of predictive in vitro, in vivo, and analytical detection methodologies of marine biotoxins stems not least from a...

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Yessotoxin induces apoptosis in HL7702 human liver cells

Cited by 5 publications

References 33 publications

Yessotoxin, a Promising Therapeutic Tool

Yessotoxin, a Promising Therapeutic Tool

Baicalein Alleviates Liver Oxidative Stress and Apoptosis Induced by High-Level Glucose through the Activation of the PERK/Nrf2 Signaling Pathway

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

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