Yersiniae other than ,  , and  : the ignored species

Sulakvelidze, Alexander

doi:10.1016/s1286-4579(00)00311-7

Cited by 138 publications

(134 citation statements)

References 87 publications

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“…Amplification products from selected positive ail and yadA samples were analysed by agarose gel electrophoresis, and Table 4 | Occurrence of Y. enterocolitica ail and yadA genes in samples from the Grand River watershed; sample locations are described in Figure 1 Number of samples Although these are considered to be non-pathogenic species, the clinical significance of this group is not fully known (Sulakvelidze 2000). We initially tested a number of reference strains isolated from clinical or porcine samples for the presence of the ail and yadA virulence genes (Table 2).…”

Section: Confirmation Of Quantitative Pcr Resultsmentioning

confidence: 99%

“…Our results also showed that both the ail and yadA virulence genes were detected in a clinical Y. intermedia strain (Table 2). Although Y. intermedia strains are considered non-pathogenic, they may have some potential for causing disease and have been isolated from symptomatic patients (Sulakvelidze 2000). In a study by (Table 2).…”

Section: Confirmation Of Quantitative Pcr Resultsmentioning

confidence: 99%

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The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Cheyne

Dyke

Anderson

et al. 2009

Journal of Water and Health

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Yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. PCR-based methods have been used to detect Y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. In this study, quantitative PCR (qPCR)-based methods targeting the Yersinia virulence genes ail and yadA were used to survey the Grand River watershed in southern Ontario, Canada. Initial testing of reference strains showed that ail and yadA PCR assays were specific for pathogenic biotypes of Y. enterocolitica; however the genes were also detected in one clinical Yersinia intermedia isolate. A survey of surface water from the Grand River watershed showed that both genes were detected at five sampling locations, with the ail and yadA genes detected in 38 and 21% of samples, respectively. Both genes were detected more frequently at colder water temperatures.A screening of Yersinia strains isolated from the watershed showed that the ail gene was detected in three Y. enterocolitica 1A/O:5 isolates. Results of this study show that Yersinia virulence genes were commonly detected in a watershed used as a source of drinking water, and that the occurrence of these genes was seasonal.

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Section: Confirmation Of Quantitative Pcr Resultsmentioning

confidence: 99%

Section: Confirmation Of Quantitative Pcr Resultsmentioning

confidence: 99%

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Cheyne

Dyke

Anderson

et al. 2009

Journal of Water and Health

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“…Y. pestis, the causative agent of plague, branched an estimated 1500 to 20,000 years ago from the closely related Y. pseudotuberculosis serotype O:1b (Achtman et al 1999). All contemporary Y. pestis isolates arose by clonal diversification from strains associated with the European black death of the 14th century (Bos et al 2011 (Sulakvelidze 2000).…”

Section: Yersinia Speciesmentioning

confidence: 99%

Host Specificity of Bacterial Pathogens

Bäumler

Fang

2013

Cold Spring Harbor Perspectives in Medicine

171

134

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“…Yersinia frederiksenii, Yersinia intermedia, Yersinia kristensenii, Yersinia bercovieri, Yersinia mollaretii, Yersinia rohdei, Yersinia ruckeri, Yersinia aldovae (Sulakvelidze, 2000) and Yersinia aleksiciae (Sprague & Neubauer, 2005). Except for Y. pestis, Yersinia species are ubiquitous and have been found in soil, fresh water sources, animals, foods and sewage (Bercovier & Mollaret, 1984;Bottone, 1997;Sulakvelidze, 2000). They have been occasionally isolated from clinical specimens (Gray, 1995) and virulence factors have been detected in several isolates of these species (Robins-Browne et al, 1991;Sulakvelidze et al, 1999;Sulakvelidze, 2000).…”

mentioning

confidence: 99%

“…Except for Y. pestis, Yersinia species are ubiquitous and have been found in soil, fresh water sources, animals, foods and sewage (Bercovier & Mollaret, 1984;Bottone, 1997;Sulakvelidze, 2000). They have been occasionally isolated from clinical specimens (Gray, 1995) and virulence factors have been detected in several isolates of these species (Robins-Browne et al, 1991;Sulakvelidze et al, 1999;Sulakvelidze, 2000). Herein we have characterized two freshwater Yersinia isolates by extensive phenotypic and molecular methods and found them to exhibit unique characteristics, suggesting that they are representative of a hitherto undescribed Yersinia species.…”

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confidence: 99%

Yersinia massiliensis sp. nov., isolated from fresh water

Merhej

Adékambi

Pagnier

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Two bacterial organisms, 50640 T and 823, were isolated from fresh water in Marseilles, France, and were further identified as members of the genus Yersinia on the basis of their phenotypic characteristics and 16S rRNA gene sequencing. Their unique phenotypic profile differed from that of closely related species of Yersinia bercovieri and Yersinia mollaretii by exhibiting positive indole and inositol tests, and from that of Yersinia frederiksenii by lacking the ability to ferment L-rhamnose. A polyphasic approach, including almost complete 16S rRNA gene sequencing (1461 bp) and partial sequencing of hsp60 (683 bp), gyrB (662 bp), sodA (624 bp) and rpoB (1049 bp) showed that isolates 50640 T and 823 exhibited 98.5, 93.5, 90.4, 92.4 and 96.6 % similarity with Y. mollaretii, 98.7, 93.0, 90.1, 89.1 and 96.2 % with Y. bercovieri, and 98.4, 93.2, 89.8, 88.9 and 95.2 % with Y. frederiksenii, respectively. Both isolates exhibited an identical 16S rRNA gene sequence and differed by one to five point mutations in housekeeping gene sequences. Phylogenetic reconstructions based on the combination of these four housekeeping genes indicated that the two isolates formed a unique branch supported by a bootstrap value of 93 %. Their unique phenotypic traits, 16S rRNA gene sequence, together with housekeeping gene sequences exhibiting ,97 % similarity with closely related species, and phylogenetic analyses suggested that the two isolates represent a so far undescribed Yersinia species. The name Yersinia massiliensis sp. nov. is proposed for this new taxon (type strain 50640 T 5CIP 109351 T 5CCUG 53443 T ; isolate 8235CIP 1093525CCUG 53444).

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Yersiniae other than , , and : the ignored species

Cited by 138 publications

References 87 publications

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Host Specificity of Bacterial Pathogens

Yersinia massiliensis sp. nov., isolated from fresh water

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