Yersinia pestis--etiologic agent of plague.

Perry, Robert D.; Fetherston, Jacqueline D.

doi:10.1128/cmr.10.1.35-66.1997

Cited by 178 publications

(328 citation statements)

References 218 publications

(676 reference statements)

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“…Therefore, it was hypothesized that these proteins may serve as protective antigens. Along with F1 and LcrV, Yops and the plasminogen activator protease (Pla) are necessary for the full virulence of Y. pestis [12,13] and are encoded by the virulence plasmids [13][14][15]. Among the 12 secreted Yops, YopB and YopD are known as translocators in the Ysc-Yop type III secretion system involved in the translocation of the Yop effectors across the eukaryotic cell plasma membrane into the target cells [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…Among the 12 secreted Yops, YopB and YopD are known as translocators in the Ysc-Yop type III secretion system involved in the translocation of the Yop effectors across the eukaryotic cell plasma membrane into the target cells [16,17]. The type III secretion apparatus of Y. pestis is a conserved mechanism to deliver virulent factors into mammalian host cells [15,18]. Both YopB and YopD are transmembrane proteins and interact with LcrV to form the pore of the Type III secretion apparatus through which the Yop effectors are translocated [13,19,20].…”

Section: Introductionmentioning

confidence: 99%

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Relative immunogenicity and protection potential of candidate Yersinia Pestis antigens against lethal mucosal plague challenge in Balb/C mice

Wang

Joshi

Mboudjeka

et al. 2008

Vaccine

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Yersinia Pestis Outer proteins, plasminogen activator protease and Yop secretion protein F are necessary for the full virulence of Yesinia pestis and have been proposed as potential protective antigens for vaccines against plague. In the current study, we used DNA immunization as a tool to study the relative protective immunity of these proteins with a standardized intranasal challenge system in mice. While the natural full length gene sequences for most of these Y. pestis proteins did not display a good level of protein expression in vitro when delivered by a DNA vaccine vector, the overall immunogenicity of these wild type gene DNA vaccines was low in eliciting antigen-specific antibody responses and gene sequence modifications improved both of these parameters. However, even modified YopD, YopO and YscF antigens were only able to partially protect immunized mice at various levels against lethal challenge with Y. pestis KIM 1001 strain while no protection was observed with either the YopB or Pla antigens. These results demonstrate that DNA immunization is effective in screening, optimizing and comparing optimal antigen designs and immunogenicity of candidate antigens for the development of a subunit-based plague vaccine.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relative immunogenicity and protection potential of candidate Yersinia Pestis antigens against lethal mucosal plague challenge in Balb/C mice

Wang

Joshi

Mboudjeka

et al. 2008

Vaccine

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“…These vaccines utilize one or both of two known protective antigens, Fraction 1 (F1) and LcrV (V). The F1 protein forms a capsule [4] and is believed to confer resistance to phagocytosis, possibly by forming aqueous pores in the membranes of phagocytic cells [5] or by interfering with complement-mediated opsonization [6]. F1 has been shown to be an effective vaccine component [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

A DNA vaccine producing LcrV antigen in oligomers is effective in protecting mice from lethal mucosal challenge of plague

Wang

Heilman

Liu

et al. 2004

Vaccine

102

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There is an urgent need to develop effective vaccines against pneumonic plague, a highly lethal and contagious disease caused by the Gram-negative bacterium Yersinia pestis. Here we demonstrate that a novel DNA vaccine expressing a modified V antigen (LcrV) of Y. pestis, with a human tissue plasminogen activator (tPA) signal sequence, elicited strong V-specific antibody responses in BALB/c mice. This tPA-V DNA vaccine protected mice from intranasal challenge with lethal doses of Y. pestis. In comparison, a DNA vaccine expressing the wild type V antigen was much less effective. Only tPA-V formed oligomers spontaneously, and elicited a higher IgG2a anti-V antibody response in immunized mice, suggesting increased TH1 type cellular immune response. Our data indicate that antigen engineering is effective in inducing high quality protective immune responses against conformationally sensitive antigens. These results support that optimized DNA vaccines have the potential to protect against bacterial pathogens than is generally recognized.

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“…Yersinia pestis, a small Gram-negative rod belonging to the family Enterobacteriaceae, is the etiological agent of plague. This organism is one of the three human pathogenic species, including Y. pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica, in the genus Yersinia [1]. Plague occurs predominantly in three different forms, that is, bubonic, pneumonic, and septicaemic, depending on the route of exposure to the pathogen.…”

Section: Introductionmentioning

confidence: 99%

“…The rapid detection of Y. pestis is critical for controlling the spread of plague. The laboratory detection of Y. pestis is based on bacteriological, serological and nucleic acid-based methods [1]. The bacterial culture is not well suitable for rapid detection of Y. pestis due to the time delay.…”

Section: Introductionmentioning

confidence: 99%

Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor

Yan

Zhou

Zhao

et al. 2006

Sensors and Actuators B: Chemical

133

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Up-converting phosphor technology (UPT)-based lateral-flow immunoassay has been developed for quantitative detection of Yersinia pestis rapidly and specifically. In this assay, 400 nm up-converting phosphor particles were used as the reporter. A sandwich immumoassay was employed by using a polyclonal antibody against F1 antigen of Y. pestis immobilized on the nitrocellulose membrane and the same antibody conjugated to the UPT particles. The signal detection of the strips was performed by the UPT-based biosensor that could provide a 980 nm IR laser to excite the phosphor particles, then collect the visible luminescence emitted by the UPT particles and finally convert it to the voltage as a signal. V T and V C stand for the multiplied voltage units for the test and the control line, respectively, and the ratio V T /V C is directly proportional to the number of Y. pestis in a sample. We observed a good linearity between the ratio and log CFU/ml of Y. pestis above the detection limit, which was approximately 10 4 CFU/ml. The precision of the intra-and inter-assay was below 15% (coefficient of variation, CV). Cross-reactivity with related Gram-negative enteric bacteria was not found. The UPT-LF immunoassay system presented here takes less than 30 min to perform from the sample treatment to the data analysis. The current paper includes only preliminary data concerning the biomedical aspects of the assay, but is more concentrated on the technical details of establishing a rapid manual assay using a state-of-the-art label chemistry.

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Yersinia pestis--etiologic agent of plague.

Cited by 178 publications

References 218 publications

Relative immunogenicity and protection potential of candidate Yersinia Pestis antigens against lethal mucosal plague challenge in Balb/C mice

Relative immunogenicity and protection potential of candidate Yersinia Pestis antigens against lethal mucosal plague challenge in Balb/C mice

A DNA vaccine producing LcrV antigen in oligomers is effective in protecting mice from lethal mucosal challenge of plague

Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor

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