Yersinia enterocolitica: a frequent seasonal stool isolate from children at an urban hospital in the southeast United States

Metchock, Beverly; Lonsway, David; Carter, G P; Lee, L A; McGowan, John E.

doi:10.1128/jcm.29.12.2868-2869.1991

Cited by 36 publications

(7 citation statements)

References 11 publications

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“…All isolates from tongue were serotype O:3 and designated SB serotype (O:3). Recent reports have confirmed the emergence of Y. enterocolitica serotype O:3 as the major cause of yersiniosis in the United States (16,17,19).…”

Section: Resultsmentioning

confidence: 90%

Use of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterocolitica of various serotypes from pork samples

Bhaduri¹,

Cottrell²,

Pickard³

1997

Appl Environ Microbiol

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Many selective enrichment methods for the isolation of Yersinia enterocolitica from foods have been described. However, no single isolation procedure has been described for the recovery and identification of various plasmid-bearing serotypes. A single improved procedure for selective enrichment, isolation, identification, and maintenance of plasmid-bearing virulent serotypes of Y. enterocolitica from pork samples was developed. Enrichment at 12°C inTrypticase soy broth containing yeast extract, bile salts, and Irgasan was found to be an efficient medium for the recovery of plasmid-bearing virulent strains of Y. enterocolitica representing O:3; O:8; O:TACOMA; O:5, O:27; and O:13 serotypes. MacConkey agar proved to be a reliable medium for the isolation of presumptive colonies, which were subsequently confirmed as plasmid-bearing virulent strains by Congo red binding and low calcium response. Further confirmation by multiplex PCR employed primers directed at the chromosomal ail and plasmid-borne virF genes, which are present only in pathogenic strains. The method was applied to pig slaughterhouse samples and was effective in isolating plasmid-bearing virulent strains of Y. enterocolitica from naturally contaminated porcine tongues. Strains isolated from ground pork and tongue expressed plasmid-associated phenotypes and mouse pathogenicity.

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Section: Resultsmentioning

confidence: 90%

Use of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterocolitica of various serotypes from pork samples

Bhaduri¹,

Cottrell²,

Pickard³

1997

Appl Environ Microbiol

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“…After incubation, 0.1 ml of the isolated colony was transferred into 9.9 ml of sterile distilled water contained in each test tube using a sterile needle and syringe, and the mixed properly. The liquid now serve as source of inoculums containing approximately 106 cfu/ml of bacterial suspension (Metchock et al, 2011, Milstone et al, 2010.…”

Section: Standardization Of Test Organismsmentioning

confidence: 99%

“…Mixture was filtered using whatman filter (No 1) papers, and the filtrates were collected directly into sterile crucibles. The filtrate was extracted using rotary evaporator, and the residues obtained were kept at room temperature (Metchock et al, 2011).…”

Section: Plant Sample-source and Collection Of Plant Samplementioning

confidence: 99%

Antimicrobial Properties, Phytochemical Composition, and Phenotypic Resistance Pattern of Selected Enteropathogenic Microorganism on Ageratum conyzoides (Goat Weed) Leaf Extract

Osuntokun¹,

Jemilaiye²,

Yusuf-Babatunde³

et al. 2018

Int. Res. J. Microbiol

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The aim of this study is to determine the phytochemical composition, antimicrobial properties and phenotypic resistance pattern of selected enteropathogenic microorganism on Ageratum conyzoides leaf extract. Enteropathogenic isolates were collected from Adekunle Ajasin University Health Centre, Akungba Akoko, Ondo State and were identified using Microbact™ 24E Identification kit. Antibiotic sensitivity testing was carried out against the enteropathogenic organism using Kirby-Bauer disc diffusion method, imipenem was active against Proteus mirabilis, Enterobacter agglomerans, Enterobacter gerogoviae, with diameter zone of inhibition of 41 mm, 26 mm and 30 mm. ofloxacin were active against Proteus mirabilis, Enterobacter agglomerans, Enterobacter gergoviae, with diameter zone of inhibition of 21, 31 and 21 mm. All enteropathogenic organisms shows high resistant rate against oxacilin, vancomycin, amoxicillin, ceftazidime, piperacillin, cefoxitin, tazobactam. Antimicrobial screening of the leaf extract of Ageratum conyzoides revealed high bioactivity against Serratia marcescens, Proteus vulgaris, Enterobacter agglomerans and Proteus mirabilis isolates with diameter zone of inhibition ranging from 15 mm to 22 mm at 100 mg/ml. Using broth dilution method, the minimum inhibitory concentrations (MIC) of the Ageratum conyzoides (Goatweed) extract range from 25 mg/ml and 100 mg/ml. The phytochemical screening results shows that Ageratum conyzoides extract contained alkaloid, pholobotannins, Cardiac glycoside, phenol, tannins, saponin, and flavonoids. It can be deduced from this research work that Ageratum conyzoides has better antimicrobial properties and it contains very important phytochemical constituents which can be a magic bullet to deal with the menace of antimicrobial resistance enteropathogenic microorganism.

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“…No statistically significant racial variation in exposure to raw pork or unpasteurized milk consumption was observed among the sub-analysis of cases reported in Washington (Table 3). 103 141 326 (16) 12 (8) 16 (27) 5 164 (27) 9 2017 (12) 13 (18) 83 123…”

Section: Resultsmentioning

confidence: 99%

The Descriptive Epidemiology of Yersiniosis: A Multistate Study, 2005–2011

et al. 2015

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This multistate analysis revealed differences in the epidemiology of yersiniosis by race/ethnicity that may be useful for future research and prevention efforts. While this study was consistent with the FoodNet report in recognizing the high and declining incidence among African American children and winter seasonality among African Americans, our study also identified April-June seasonality among the white population.

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Yersinia enterocolitica: a frequent seasonal stool isolate from children at an urban hospital in the southeast United States

Cited by 36 publications

References 11 publications

Use of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterocolitica of various serotypes from pork samples

Use of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterocolitica of various serotypes from pork samples

Antimicrobial Properties, Phytochemical Composition, and Phenotypic Resistance Pattern of Selected Enteropathogenic Microorganism on Ageratum conyzoides (Goat Weed) Leaf Extract

The Descriptive Epidemiology of Yersiniosis: A Multistate Study, 2005–2011

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