Yellow Lupine Cyclophilin Transcripts Are Highly Accumulated in the Nodule Meristem Zone

Nuc, Katarzyna; Nuc, Przemysław; Słomski, Ryszard

doi:10.1094/mpmi.2001.14.12.1384

Cited by 17 publications

(14 citation statements)

References 68 publications

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“…Due to the presence of a high number of small vacuoles in meristem cells, vacuolar proteins such as endochitinase were detected. Also cyclophilin (peptidyl-prolyl cis – trans isomerase) is a highly abundant protein in meristematic tissue (Nuc et al 2001). Again, mainly low molecular mass proteins were identified and no correlation between the different physico-chemical parameters could be demonstrated.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of chloroform/methanol extraction to facilitate the study of membrane proteins of non-model plants

Vertommen

Panis

Swennen

et al. 2010

Planta

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Membrane proteins are of great interest to plant physiologists because of their important function in many physiological processes. However, their study is hampered by their low abundance and poor solubility in aqueous buffers. Proteomics studies of non-model plants are generally restricted to gel-based methods. Unfortunately, all gel-based techniques for membrane proteomics lack resolving power. Therefore, a very stringent enrichment method is needed before protein separation. In this study, protein extraction in a mixture of chloroform and methanol in combination with gel electrophoresis is evaluated as a method to study membrane proteins in non-model plants. Benefits as well as disadvantages of the method are discussed. To demonstrate the pitfalls of working with non-model plants and to give a proof of principle, the method was first applied to whole leaves of the model plant Arabidopsis. Subsequently, a comparison with proteins extracted from leaves of the non-model plant, banana, was made. To estimate the tissue and organelle specificity of the method, it was also applied on banana meristems. Abundant membrane or lipid-associated proteins could be identified in both tissues, with the leaf extract yielding a higher number of membrane proteins.Electronic supplementary materialThe online version of this article (doi:10.1007/s00425-010-1121-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Evaluation of chloroform/methanol extraction to facilitate the study of membrane proteins of non-model plants

Vertommen

Panis

Swennen

et al. 2010

Planta

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“…CYPs have PPIase activity that catalyzes the cis-trans isomerization of proline residues in polypeptides (Fischer et al 1989;Liu et al 1990;Takahashi et al 1989). Plant CYPs were first reported in tomato, maize, and rape (Gasser et al 1990) and many Cyp genes have since been isolated and characterized in a variety of plant species (Gasser et al 1990;Buchholz et al 1994;Marivet et al 1994Marivet et al , 1995Kullertz et al 1999;Saito et al 1999;Nuc et al 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Agrobacterium tumefaciens strain EHA105 carrying the LeCyp1 gene construct was used to transform hypocotyl sections of 2-week-old light-grown dgt 1-1 seedlings as described (Knapp et al 1994). Transformed shoots were selected on Murashige and Skoog (Nuc et al 2001) medium supplemented with Gamborg vitamin B5 mix (Gamborg et al 1968), 5 lM 6-benzyladenine, 0.5 lM 1-NAA, and 0.025-0.1% (w/v) glufosinate ammonium (BASTA; Fluka, Milwaukee, WI). Regenerated shoots were rooted on phytohormone-free selection medium then transplanted to soil.…”

Section: Identification Of the Dgt Genementioning

confidence: 99%

The diageotropica gene of tomato encodes a cyclophilin: a novel player in auxin signaling

Ivanchenko

White

et al. 2006

Planta

114

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The single gene, auxin-resistant diageotropica (dgt) mutant of tomato displays a pleiotropic auxin-related phenotype that includes a slow gravitropic response, lack of lateral roots, reduced apical dominance, altered vascular development, and reduced fruit growth. Some auxin responses are unaltered in dgt plants, however, and the levels, metabolism, and transport of auxin appear normal, indicating that the Dgt gene encodes a component of a specific auxin signaling pathway. By combining map-based cloning with comparative microsynteny, we determined that the Dgt gene encodes a cyclophilin (CYP) (LeCYP1; gi:170439) that has not previously been identified as a component of auxin signaling and plant development. Each of the three known dgt alleles contains a unique mutation in the coding sequence of LeCyp1. Alleles dgt(1-1)and dgt(1-2) contain single nucleotide point mutations that generate an amino acid change (G137R) and a stop codon (W128stop), respectively, while dgt(dp) has an amino acid change (W128CDelta129-133) preceding a 15 bp deletion. Complementation of dgt plants with the wild-type LeCyp1 gene restored the wild-type phenotype. Each dgt mutation reduced or nullified the peptidyl-prolyl isomerase activity of the GST-LeCYP1 fusion proteins in vitro. RT-PCR and immunoblot analyses indicated that the dgt mutations do not affect the expression of LeCyp1 mRNA, but the accumulation of LeCYP1 protein is greatly reduced for all three mutant alleles. The CYP inhibitor, cyclosporin A, partially mimics the effects of the dgt mutation in inhibiting auxin-induced adventitious root initiation in tomato hypocotyl sections and reducing the auxin-induced expression of the early auxin response genes, LeIAA10 and 11. These observations confirm that the PPIase activity of the tomato CYP, LeCYP1, encoded by the Dgt gene is important for specific aspects of auxin regulation of plant growth, development, and environmental responses.

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“…AtROC1 shared a maximum of 86% identity with AdCyp. A cyclophilin from a legume (Lupinus luteus) LlCyp, which was shown to be induced during nodulation (Nuc et al 2001) showed 84% identity. AdCyp homologs from human and yeast, HsCypA and ScCPR1 showed 75% and 69% identity respectively.…”

Section: Multiple Sequence Alignment and Phylogenetic Analysismentioning

confidence: 99%

Differential gene expression in Arachis diogoi upon interaction with peanut late leaf spot pathogen, Phaeoisariopsis personata and characterization of a pathogen induced cyclophilin

Kumar

Kirti

2011

Plant Mol Biol

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The wild relatives of peanut are resistant to various economically important diseases including late leaf spot (LLS) caused by Phaeoisariopsis personata, compared with the susceptible cultivated peanut (Arachis hypogaea L.). The interaction of the late leaf spot pathogen, Phaeoisariopsis personata and the highly resistant, diploid peanut wild species, Arachis diogoi was analyzed at the molecular level by differential gene expression studies. Genes up-regulated with in 48 h of pathogen challenge were isolated as partial cDNAs. Some of the isolated genes, which are shown to be involved in the first line of defense in plants, were further characterized with respect to their transcriptional regulation in response to pathogen. Among the isolated clones, two were found to encode cyclophilin like proteins. One of the two isolated partial cDNAs encoding cyclophilin like proteins was extended using 5' RACE. The full length cDNA, designated as AdCyp, was 886 bp in length and encodes a polypeptide of 172 amino acids. Southern hybridization suggests that AdCyp is possibly coded by a single gene and at least one more identical gene is present in Arachis diogoi genome. AdCyp exhibits evolutionary conservation across the kingdoms. Phylogenetic analysis showed that AdCyp belongs to the subgroup I of Group I in cyclophilins. A translational fusion of GFP-AdCyp was found to localize to both cytosol and nucleus. AdCyp transcripts were found to accumulate in response to the treatments with pathogen as well as phytohormones. Constitutive heterologous expression of AdCyp resulted in enhanced resistance to Ralstonia solanacearum and reduced susceptibility towards Phytophthora parasitica var. nicotianae in transgenic tobacco and the resistance was associated with higher transcript levels of various defense related genes.

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Yellow Lupine Cyclophilin Transcripts Are Highly Accumulated in the Nodule Meristem Zone

Cited by 17 publications

References 68 publications

Evaluation of chloroform/methanol extraction to facilitate the study of membrane proteins of non-model plants

Evaluation of chloroform/methanol extraction to facilitate the study of membrane proteins of non-model plants

The diageotropica gene of tomato encodes a cyclophilin: a novel player in auxin signaling

Differential gene expression in Arachis diogoi upon interaction with peanut late leaf spot pathogen, Phaeoisariopsis personata and characterization of a pathogen induced cyclophilin

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