Yeasts producing zeatin

Abstract

The present paper describes the first screening study of the ability of natural yeast strains to synthesize in culture the plant-related cytokine hormone zeatin, which was carried out using HPLC-MS/MS. A collection of 76 wild strains of 36 yeast species (23 genera) isolated from a variety of natural substrates was tested for the production of zeatin using HPLC-MS/MS. Zeatin was detected in more than a half (55%) of studied strains and was more frequently observed among basidiomycetous than ascomycetous species. The amount of zeatin accumulated during the experiment varied among species and strains. Highest zeatin values were recorded for basidiomycete Sporobolomyces roseus and ascomycete Taphrina sp. that produced up to 8,850.0 ng and 5,166.4 ng of zeatin per g of dry biomass, respectively. On average, the ability to produce zeatin was more pronounced among species isolated from the arctic-alpine zone than among strains from tropical and temperate climates. Our study also demonstrated that epiphytic strains and pigmented yeast species, typically for phyllosphere, are able to more often produce a plant hormone zeatin than other yeasts.

The Origins and Roles of Methylthiolated Cytokinins: Evidence From Among Life Kingdoms

“…Mod5, a yeast tRNA-isopentenyltransferase, is also involved in protein synthesis and sporulation (Laten et al, 1978) and Mod5 mutants displayed reduced resistance to antifungal agents (Suzuki et al, 2012). More recently, a survey confirmed production of zeatin-type CKs in a wide range of yeast species (Streletskii et al, 2019); however, the authors did not resolve their data between the trans-and cis-isomers.…”

Shaping the leaf microbiota: plant–microbe–microbe interactions

“…A stressed and nutrient-poor condition of the leaf surface makes this environment selective to certain microorganisms. Hence, different microbial mechanisms such as ability to extract nutrients, produce hormones and surfactants, as well as motility and biofilm formation can be key to colonization success ( Nadakuduti et al , 2012 ; Ueda et al , 2018 ; Leveau, 2019 ; Oso et al , 2019 ; Streletskii et al , 2019 ). Most epiphytes survive on the leaf surface by forming large aggregates which help them to cope with the surrounding milieu and maintain a hydrated surface by production of extracellular polymeric substances (EPSs) ( Morris and Kinkel, 2002 ; Lindow and Brandl, 2003 ; Baldotto and Olivares, 2008 ; Vorholt, 2012 ).…”

Culturable Yeasts as Biofertilizers and Biopesticides for a Sustainable Agriculture: A Comprehensive Review

“…Zeatin is one of the most common cytokinins, which has been reported to be synthetized by some yeasts such as Sporobolomyces roseus , Metschnikowia pulcherrima and A. pullulans . As with auxins, zeatin synthesis in culture varies among species and strains, and may depend on culture conditions [ 94 ]. Additionally, gibberellins, commonly known as gibberellic acids, are another important plant growth regulator.…”