Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2

Abstract

Transforming growth factor β-stimulated clone 22 domain family, member 2 (TSC22D2), a member of the TSC22D family, has been implicated as a tumor-associated gene, but its function remains unknown. To further explore its biological role, yeast two-hybrid screening combined with multiple bioinformatics tools was used to identify 44 potential interacting partners of the TSC22D2 protein that were mainly involved in gene transcription, cellular metabolism, and cell cycle regulation. The protein WD repeat domain 77 (WDR77) was selected for further validation due to its function in the cell cycle and tumor development, as well as its high detection frequency in the yeast two-hybrid assay. Immunoprecipitation and immunofluorescence experiments confirmed an interaction between the TSC22D2 and WDR77 proteins. Our work greatly expands the putative protein interaction network of TSC22D2 and provides deeper insight into the biological functions of the TSC22D2 and WDR77 proteins.

A network-based approach to identify DNA methylation and its involved molecular pathways in testicular germ cell tumors

“…Notably, KEGG pathway enrichment analysis suggested significant enrichment in pathways including ECM-receptor interaction, microRNA in cancer [45][46][47][48] and PI3K-Akt signaling pathway. The previous studies reported that PI3K-Akt signaling activation is associated with tumor cell growth, proliferation, migration and angiogenesis of TGCT [49][50][51][52]. It is well known that increased cell proliferation and migration are the leading causes for malignant tumor initiation and progression [53][54][55], thus our biological analysis results were consistent with previous researches.…”

Co-expression of AFAP1-AS1 and PD-1 predicts poor prognosis in nasopharyngeal carcinoma

“…Mining of published high-throughput data is a commonly used and low-cost method for identifying novel biomarkers and gaining insight into the biological functions of novel genes [21–23]. To identify potential novel functions of AFAP1-AS1 , we downloaded a GEP dataset, GSE12452, from the GEO database; this dataset consists of 41 samples of whole-genome GEP data, including 10 samples of non-tumor nasopharyngeal epithelial (NPE) biopsies and 31 cases of NPC [24].…”

High Expression of LINC01420 indicates an unfavorable prognosis and modulates cell migration and invasion in nasopharyngeal carcinoma

“…Tissue RNA isolation and amplification were performed as our laboratory described previously 24-26. Cell RNA was extracted using TRIzol reagent (Invitrogen, Carlbad, CA, USA).…”

“…The bottom well contained growth medium with 20 % FBS. Cells were incubated at 37°C for 24 h. After 24 h, the cells that had migrated to the lower face of the filters were fixed with 100% methanol and stained with 0.5% crystal violet and counted 24, 25.…”