“…While TF-centered approaches such as ChIP-seq can reveal the extent to which a particular TF is involved in genome-wide regulation, other techniques such as high-throughput yeast one-hybrid (HT-Y1H) are promoter-focused and can provide a survey of potential interactors for a single promoter. Reagents for the latter have been developed and applied successfully to study gene regulation in humans, flies ( Drosophila melanogaster ) and worms ( Caenorhabditis elegans ), and are starting to reveal the complexity of combinatorial gene regulation (Deplancke et al, 2004; Hens et al, 2011; Reece-Hoyes et al, 2011). Recently, we implemented this genomic strategy in plants ( Arabidopsis thaliana , Arabidopsis) and identified novel transcriptional components of the circadian clock (Pruneda-Paz et al, 2009) as well as other plant physiological processes (Ito et al, 2012; Li et al, 2012; Niwa et al, 2013).…”