Yeast Hog1 proteins are sequestered in stress granules during high-temperature stress

Shiraishi, Kosuke; Hioki, Takahiro; Habata, Akari; Yurimoto, Hiroya; Sakai, Yasuyoshi

doi:10.1242/jcs.209114

Cited by 11 publications

(4 citation statements)

References 36 publications

(46 reference statements)

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“…Therefore, it is possible that weak Hog1 phosphorylation resulted in irreversible activation of different cellular signaling processes in the cytoplasm. Recently, cytosolic localization of Hog1 was also reported following heat stress (Shiraishi et al, 2017). Among the interacting partners of the dynactin complex, Bzz1, but none of the other interacting Fig.…”

Section: Discussionmentioning

confidence: 94%

Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint

Sukegawa

Negishi

Kikuchi

et al. 2018

Journal of Cell Science

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The cell wall integrity checkpoint monitors synthesis of cell wall materials during the cell cycle. Upon perturbation of cell wall synthesis, the cell wall integrity checkpoint is activated, downregulating Clb2 transcription. Here, we identified genes involved in this checkpoint by genetic screening of deletion mutants. In addition to the previously identified dynactin complex, the Las17 complex, in particular the Bzz1 and Vrp1 components, plays a role in this checkpoint. We also revealed that the high osmolarity glycerol (HOG) and cell wall integrity mitogen-activated protein kinase (MAPK) signaling pathways are essential for checkpoint function. The defective checkpoint caused by the deficient dynactin and Las17 complexes was rescued by hyperactivation of the cell wall integrity MAPK pathway, but not by the activated form of Hog1, suggesting an order to these signaling pathways. Mutation of Fkh2, a transcription factor important for Clb2 expression, suppressed the checkpoint-defective phenotype of Las17, HOG MAPK and cell wall integrity MAPK mutations. These results provide genetic evidence that signaling from the cell surface regulates the downstream transcriptional machinery to activate the cell wall integrity checkpoint.

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Section: Discussionmentioning

confidence: 94%

Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint

Sukegawa

Negishi

Kikuchi

et al. 2018

Journal of Cell Science

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“…SGs have been proposed to intercept and sequester signaling components 26 . Exposure of Candida boidinii, Pichia pastoris and Schizosaccharomyces pombe to high-temperature stress resulted in colocalization of the CbHog1 with the stress granule marker Pab1 27 . In other cases, Hog1 was found to affect cellular processes linked to SG formation, though indirectly 28 .…”

Section: Discussionmentioning

confidence: 99%

Cytoplasmic sequestering of a fungal stress-activated MAPK in response to a host plant phenolic acid

Horwitz

Zuchman

Koren

et al. 2023

Preprint

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The stress-activated MAPK Hog1 in Cochliobolus heterostrophus, a maize foliar pathogen, undergoes dephosphorylation upon exposure to ferulic acid (FA), a phenolic compound abundant in the host plant. Unlike its nuclear localization during osmotic stress, Hog1:GFP forms cytoplasmic foci in response to FA, indicating its sequestering. By using Hog1:GFP as an affinity purification bait, we isolated an FA-dependent sub-proteome from a subcellular fraction enriched with fluorescent foci. The identified proteins include RNA-binding proteins, translation initiation factors and mitochondrial proteins, suggesting the foci to be stress granules. Notably, the RRM and pumilio domain protein Puf2 was enriched, and Puf2:tdTomato formed foci partially colocalizing with Hog1:GFP foci. Hog1:GFP did not colocalize with peroxisomes. Mitochondria, however, which rapidly fragmented upon FA exposure, are partially overlapped with Hog1:GFP foci. The sequestering and dephosphorylation of MAPK Hog1 may collectively attenuate cell death induced by defense compounds released by the plant host.

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“…It is of note that SGs (i.e., cytoplasmatic granules marked by Pab1/PABP and Pbp1/Ataxin‐2) have also been observed during thermal stress (i.e., 42°C for 30 min) in Candida boidinii and Pichia pastoris (Shiraishi et al, 2018).…”

Section: Other Types Of Stress That Promote Sg Formationmentioning

confidence: 99%

Yeast stress granules at a glance

Groušl

Vojtová

Hašek

et al. 2021

Yeast

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The formation of stress granules (SGs), membrane-less organelles that are composed of mainly messenger ribonucleoprotein assemblies, is the result of a conserved evolutionary strategy to cellular stress. During their formation, which is triggered by robust environmental stress, SGs sequester translationally inactive mRNA molecules, which are either forwarded for further processing elsewhere or stored during a period of stress within SGs. Removal of mRNA molecules from active translation and their sequestration in SGs allows preferential translation of stress response transcripts. By affecting the specificity of mRNA translation, mRNA localization and stability, SGs are involved in the overall cellular reprogramming during periods of environmental stress and viral infection. Over the past two decades, we have learned which processes drive SGs assembly, how their composition varies under stress, and how they co-exist with other subcellular organelles. Yeast as a model has been instrumental in our understanding of SG biology. Despite the specific differences between the SGs of yeast and mammals, yeast have been shown to be a valuable tool to the study of SGs in translation-related stress response. This review summarizes the data surrounding SGs that are formed under different stress conditions in Saccharomyces cerevisiae and other yeast species. It offers a comprehensive and up-to-date view on these still somewhat mysterious entities.

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Yeast Hog1 proteins are sequestered in stress granules during high-temperature stress

Cited by 11 publications

References 36 publications

Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint

Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint

Cytoplasmic sequestering of a fungal stress-activated MAPK in response to a host plant phenolic acid

Yeast stress granules at a glance

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