Yeast gDNA extraction v1

Abstract

The purpose of this protocol is to describe how to purify genomic (or chromosomal) DNA from yeast. Here, we use Saccharomyces cerevisiae strain DBY10418, though this protocol should apply to other yeast strains. This protocol is adapted from many other protocols (for example, see "Molecular genetic analysis of fission yeast Schizosaccharomyces pombe" by Sergio Moreno, Amar Klar, and Paul Nurse in Methods in Enzymology Volume 194, 1991, Pages 795-823, section: Preparing Schizosaccharomyces pombe Chromosomal DNA) but we made enough changes to those protocols that made it necessary to publish our own. We used the downstream DNA for whole genome sequencing with Illumina technology, but we anticipate the genomic DNA isolated in this manner can be used for other applications. This protocol uses Zymolyase to digest the yeast cell wall, SDS to lyse the cells, potassium acetate to precipitate proteins, and purifies the resulting genomic DNA via alcohol precipitations and RNase A digestion.