Yeast gain-of-function mutations reveal structure–function relationships conserved among different subfamilies of transient receptor potential channels

Su, Zhenwei; Zhou, Xian; Haynes, W. John; Loukin, Stephen H.; Anishkin, Andriy; Saimi, Yoshiro; Kung, Ching

doi:10.1073/pnas.0708584104

Cited by 39 publications

(48 citation statements)

References 41 publications

(61 reference statements)

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“…The A419P mutation in TRPML3 is causing a varitint-waddler phenotype with deafness and skin pigmentation defects in mice (39). The F380L mutation in the yeast yvc1 (yeast vacuolar conductance 1) causes stronger responses upon addition of osmotic shock-induced Ca 2ϩ release from the vacuole into the cytosol (22,40).…”

Section: Discussionmentioning

confidence: 99%

“…Linker Alignment-Several gain-of-function mutations within the S4 -S5 linker of TRP genes have been identified, including the yeast Yvc1 F380L (22), the Drosophila TRP F550I (23), the human TRPV4 R594H, R616Q, and V620I (24,25), the human, mouse, and rat TRPV3 G573S, and G573C (26 -29), and the mouse TRPC3 T635A mutations (30), which are indicated in red in Fig. 1.…”

Section: S4 -S5mentioning

confidence: 99%

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Conserved Gating Elements in TRPC4 and TRPC5 Channels

Beck

Speicher

Stoerger

et al. 2013

Journal of Biological Chemistry

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Background: Gating mechanisms of TRPC channels are mostly unknown. Results: Replacing the highly conserved glycine residue within the linker between transmembrane domains 4 and 5 by serine renders TRPC4 and TRPC5 channels constitutively active. Conclusion: TRPC channel opening seems to require similar constraints than the voltage-gated potassium channels. Significance: Novel mechanistic insights into structural requirements of TRPC channel gating are provided.

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Section: Discussionmentioning

confidence: 99%

Section: S4 -S5mentioning

confidence: 99%

Conserved Gating Elements in TRPC4 and TRPC5 Channels

Beck

Speicher

Stoerger

et al. 2013

Journal of Biological Chemistry

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“…A BLAST search using the homolog sequence against human proteins produced TRPC6, -7, and -3 as the highest scoring hits, with 23% sequence identity (among 280 core residues) with TRPC6. The important residues for TRPY1 channel gating that are conserved in other TRP channels, such as Phe 380 in the putative TM5 and Tyr 458 in the putative TM6 (58), are also conserved in this homolog (Fig. 1).…”

Section: Trpgz Is a Trp Channel Homolog In The Fungal Vacuolar Membramentioning

confidence: 99%

Molecular Bases of Multimodal Regulation of a Fungal Transient Receptor Potential (TRP) Channel

Ihara

Hamamoto

Miyanoiri

et al. 2013

Journal of Biological Chemistry

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Background: Multimodality of TRP channels underlies their diverse physiological functions. Results: We identified a fungal multimodal TRP channel whose cytosolic domain (CTD) mediates various channel regulation. Conclusion: CTD has an oligomerization module critical for osmoreception, yet its flexible structure allows dynamic regulations with other functional modalities. Significance: This work proposes structural and biophysical principles for multimodality of a TRP channel family member.

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“…The combination of these two ways of observing TRPY1 activities makes it a useful model for MS-TRP channels and for eukaryotic primary force transducers. In addition, TRPY1 exhibits a large 300-pS signal over noise, requires no heterologous expression [39], and can easily be manipulated by molecular genetics [48,59], facilitating the sorts of experiments that are practical with yeast genetics but difficult with animals.…”

Section: Introductionmentioning

confidence: 99%

The use of yeast to understand TRP-channel mechanosensitivity

Su¹,

Loukin²,

Haynes³

et al. 2009

Pflugers Arch - Eur J Physiol

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Mechanosensitive (MS) ion channels likely underlie myriad force-sensing processes, from basic osmotic regulation to specified sensations of animal hearing and touch. Albeit important, the molecular identities of many eukaryotic MS channels remain elusive, let alone their working mechanisms. This is in stark contrast to our advanced knowledge on voltage- or ligand-sensitive channels. Several members of transient receptor potential (TRP) ion channel family have been implicated to function in mechanosensation and are recognized as promising candidate MS channels. The yeast TRP homolog, TRPY1, is clearly a first-line force transducer. It can be activated by hypertonic shock in vivo and by membrane stretch force in excised patches under patch clamp, making it a useful model for understanding TRP channel mechanosensitivity in general. TRPY1 offers two additional research advantages: (1) It has a large ( approximately 300 pS) unitary conductance and therefore a favorable S/N ratio. (2) Budding yeast allows convenient and efficient genetic and molecular manipulations. In this review, we focus on the current research of TRPY1 and discuss its prospect. We also describe the use of yeast as a system to express and characterize animal TRP channels.

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Yeast gain-of-function mutations reveal structure–function relationships conserved among different subfamilies of transient receptor potential channels

Abstract: mechanosensitivity ͉ mechanosensor ͉ aromatic residues ͉ single channel

Cited by 39 publications

References 41 publications

Conserved Gating Elements in TRPC4 and TRPC5 Channels

Conserved Gating Elements in TRPC4 and TRPC5 Channels

Molecular Bases of Multimodal Regulation of a Fungal Transient Receptor Potential (TRP) Channel

The use of yeast to understand TRP-channel mechanosensitivity

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