Yeast cloning and biotechnology

Walker, John M.; Rapley, Ralph; Curran, Brendan; Bugeja, Virginia

doi:10.1039/9781847551498-00155

Cited by 3 publications

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“…Different fungal species respond to oxygen availability in diverse ways and Table 1. 6. categorizes fungi into different groups on this basis.…”

Section: Cell Envelopementioning

confidence: 99%

“…The macrolide ring contains 12-37 carbon atoms; the conjugated double-bond structure is contained exclusively within the cyclic lactone. A number of hydroxyl groups (6)(7)(8)(9)(10)(11)(12)(13)(14) are distributed along the macrolide ring on alternate carbon atoms. Amphotericin B has a free carboxyl group and a primary amine group that confer amphoteric properties on the compound, hence the drug's name.…”

Section: General Properties Of Polyenesmentioning

confidence: 99%

“…A 4. 6 The synthesis of antibiotics by fungi can be affected by the nature of the fermentation system, the level of nitrogen or phosphate in the culture medium and feed-back inhibition where the antibiotic inhibits its own synthesis.…”

Section: Booksmentioning

confidence: 99%

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Fungal Genetics

Whiteway¹,

Bachewich²

2005

Fungi

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“…Different fungal species respond to oxygen availability in diverse ways and Table 1. 6. categorizes fungi into different groups on this basis.…”

Section: Cell Envelopementioning

confidence: 99%

Section: General Properties Of Polyenesmentioning

confidence: 99%

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Fungal Genetics

Whiteway¹,

Bachewich²

2005

Fungi

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Conclusion

2015

DNA Engineered Noble Metal Nanoparticles

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et al. 2001

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The recovery of cutinase of Fusarium solani pisi produced by the yeast Saccharomyces cerevisiae was studied in a fluidised bed adsorption system directly integrated with a productive fermenter (so-called direct product sequestration; DPS). The relative efficiency of this system was compared with the one of a conventional purification process by discrete sequences of fermentation, broth clarification, ultrafiltration and fixed bed anion exchange chromatography. By direct product sequestration of the extracellular heterologous cutinase it was possible, through only one unit operation: (i) to perform broth clarification, (ii) to obtain a high cutinase concentration factor, and (iii) to recover cutinase with a specific activity that equalled that obtained with the conventional purification process. It was also possible (iv) to substantially reduce the total process time, (v) to improve the overall yield, and (vi) to increase cutinase productivity. Furthermore, the procedure outlined is suitable for large scale bioprocess exploitation.

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Yeast cloning and biotechnology

Cited by 3 publications

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Fungal Genetics

Fungal Genetics

Conclusion

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