Yeast Cells with an Artificial Mineral Shell: Protection and Modification of Living Cells by Biomimetic Mineralization

Silicification‐Induced Cell Aggregation for the Sustainable Production of H2 under Aerobic Conditions

“…[11] Recent achievements suggest that biomimetic mineralization can be developed as auseful tool to modify cells and viruses,and the resulting cell-material complexes always have different biological characteristics from the native ones. [12] Herein we report ab iomimetic silicification that can confer green alga with an ew capacity for sustainable photobiological H 2 production under natural atmospheric conditions.…”

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

“…Recently,a rtificial nanocoatings have appeared as an approach to mimic the robustness of cell membranes and walls found in some extremophiles,while avoiding genetic modification. [7][8][9] Nanocoating cell surfaces creates ap hysical barrier between the cell and the external environment while still allowing nutrient exchange.T hese nanocoatings have improved cellular tolerance against heat, [10,11] UV radiation, [12,13] toxins, [14][15][16][17] osmotic pressure, [18,19] and mechanical stress. [20,21] Although nanocoatings have demonstrated protective abilities,t heir integration with active biomacromolecules is ac hallenge.T his prevents the bioengineering of such coatings with extrinsic bioactive functionalities that could impart artificial adaptive ability, thus overcoming original biological limitations.F or example, eukaryotic cells lack the ability to harvest energy from nutrient-depleted environments; [22] however, providing cells with abioactive protective nanocoating capable of converting depleted media into usable nutrients could furnish new opportunities in therapy, [23] diagnostics, [24] stressresponse, [25,26] and biocatalysis, [27] and could also revolutionize the dairy and pharmaceutical industries.…”

Interfacing Living Yeast Cells with Graphene Oxide Nanosheaths

“…The multilayer deposition was initiated with positively charged GO-NH þ 3 nanosheet to enhance the electrostatic interaction because the native yeast cells were known to present highly negative charge. [15][16][17] It is obvious that deposition of oppositely charged GO nanosheet would change the surface charge of the yeast cells. The surface charge, therefore, was individually measured with the progress of the deposition of GO-NH þ 3 and GO-COOnanosheets on the yeast cells from the first layer to the fifth layer ( Figure 1a).…”

“…Organic polymers and inorganic nanoparticles can bring various new functionalities to the cell membranes, including fluorescent and magnetic property, catalytic moieties, and supporting templates. [11][12][13][14][15][16][17][18][19] For that, we have attempted to assemble multilayers on the yeast cells with a combination of GO nanosheets with two most common strong polyelectrolytes such as PDDA þ , and PSS -. According to the same protocol, the yeast cells were encapsulated based on the electrostatic interactions between oppositely charged GO nanosheets and organic polyelectrolytes in a format of yeast@(PDDA þ /GO-COO -) and yeast@(GO-NH þ 3 / PSS -), respectively.…”

“…[3][4][5][6][7][8][9] In addition, the surface of microbial cells was doped with various nanoparticles, such as silica, [10,11] gold, [12] silver, and iron, [13] and carbon nanotubes [14] by the LbL self-assembly. Very recently, individual yeast cells were encapsulated within silica, [15,16] calcium phosphate, [17] calcium carbonate, [18] and polydopamine, [19] inspired by biomineralization processes or an adhesive protein found in mussels. On the other hand, a new strategy to the modification of cell surfaces by in vivo synthesis of diverse nanoparticles has been reported for certain types of bacterial cells [20] or recombinant bacterial cells.…”